Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Polymorphism of Bovine Lymphocyte Antigen DRB3.2 Alleles in Iranian Native Sarabi Cows

  • Pashmi, M. (Department of Animal Science, Abhar Azad University) ;
  • Ghorashi, S.A. (National Institute for Genetic Engineering and Biotechnology) ;
  • Salehi, A.R. (Department of Animal Science, University of Tehran) ;
  • Moini, M. (Department of Animal Science, Abhar Azad University) ;
  • Javanmard, A. (West and North-West Agricultural Biotechnology, Genomics Dept.) ;
  • Qanbari, S. (Research Institute of Agricultural Physiology & Biotechnology, University of Zanjan) ;
  • Yadranji-Aghdam, S. (Faculty of Biology, University of Tehran)
  • Received : 2005.07.17
  • Accepted : 2005.12.19
  • Published : 2006.06.01
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Abstract

Sarabi cows (n = 136) from the Sarabi Breeding Station were genotyped at bovine lymphocyte antigen (BoLA)-DRB3.2 locus by a genotyping system that used the polymerase chain reaction and restriction fragment length polymorphism. Genomic DNA was extracted from whole blood samples. A two-step polymerase chain reaction was carried out in order to amplify a 284 base-pair fragment of target gene. Nested-PCR products were digested with three restriction endonuclease enzymes RsaI, BstYI and HaeIII. Digested fragments were analyzed by polyacrylamide gel electrophoresis. Twenty-six BoLA-DRB3.2 alleles were identified with frequencies ranging from 0.4 to 15.1%. Six new allele types observed in this study have not been reported previously. Identified alleles include: BoLA-DRB3.$2^*1$, $^*2$, $^*4$, $^*6$, $^*8$, $^*12$, $^*13$, $^*14$, $^*15$, $^*16$, $^*17$, $^*23$, $^*24$, $^*25$, $^*28$, $^*32$, $^*34$, $^*35$, $^*36$, $^*37$, $^*42$, $^*46$, $^*51$, $^*kba$, $^*laa$ and $^*vaa$. Their frequencies were found to be 0.4, 0.4, 0.7, 11.4, 1.1, 1.8, 2.9, 2.2, 4.4, 9.6, 1.1, 13.6, 0.4, 0.4, 1.1, 0.7, 0.4, 6.2, 2.2, 3.7, 1.1, 7.7, 1.5, 15.1, 2.6 and 7.3% respectively. The six most frequent alleles (DRB3.2 $^*6$, $^*16$, $^*23$, $^*46$, $^*kba$ and $^*vaa$) accounted for 64.7% of the alleles in the population of this herd. Numerous studies on this locus, covering different breeds, has revealed the existence of various alleles in this locus, and new investigations have introduced novel alleles. With respect to the high number of the observed alleles in this survey and the novelty of some alleles with no previous record of reporting, it is plausible to conclude that the BoLA-DRB3.2 locus is highly polymorphic in Iranian native Sarabi cows.

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References

  1. Dietz, A. B., J. C. Detilleux, A. E. Freeman, D. H. Kelly, J. R. Stabel and M. E. Kehrli. Jr. 1997a. Genetic association of bovine lymphocyte antigen DRB3 alleles with immunological traits of Holstein cattle. J. Dairy Sci. 80:400-405 https://doi.org/10.3168/jds.S0022-0302(97)75950-2
  2. Dietz, A. B., N. D. Cohen, L. Timms and M. E. Kehrli. Jr. 1997b. Bovine Lymphocyte antigen class II alleles as risk factors for high somatic cell counts in milk of lactating dairy cows. J. Dairy Sci. 80:406-412 https://doi.org/10.3168/jds.S0022-0302(97)75951-4
  3. Gelhaus, A., L. Schnittger, D. Mehlitz, R. D. Horstmann and C. G. Meyer. 1995. Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles. Anim. Genet. 26:147-153 https://doi.org/10.1111/j.1365-2052.1995.tb03154.x
  4. Gilliespie, B. E., B. M. Jayarao, H. H. Dowlen and S. P. Oliver. 1999. Analysis and frequeacy of bovine lymphocyte antigen DRB3.2 alleles in Jersey cows. J. Dairy Sci. 82:2049-2053 https://doi.org/10.3168/jds.S0022-0302(99)75443-3
  5. Kelm, S. C., J. C. Deitilleux, A. E. Freeman, M. E. Kehrli. Jr, A. B. Dietz, L. K. Fox, J. E. Butler, I. Kasckovics and D. H. Kelley 1997. Genetic association between parameters of innate immunity and measures of mastitis in periparturient Holstein cattle. J. Dairy Sci. 80:1767-1775 https://doi.org/10.3168/jds.S0022-0302(97)76110-1
  6. Ledwidge, S. A., B. A. Mallard, J. P. Gibson, G. B. Jansen and Z. H. Jiang. 2001. Multi-primer target PCR for rapid identification of bovine DRB3 alleles. Anim. Genet. 32:219- 221 https://doi.org/10.1046/j.1365-2052.2001.00761.x
  7. Lewin, H. A. 1989. Disease resistance and immune response genes in cattle: Strategies for their detection and evidence of their existence. J. Dairy Sci. 72:1334-1348 https://doi.org/10.3168/jds.S0022-0302(89)79241-9
  8. Lewin, H. A. 1994. Host genetic mechanism of resistance and susceptibility to a bovine retroviral infection. Anim. Biotech. 5:183-191 https://doi.org/10.1080/10495399409525820
  9. Lewin, H. A. 1996. Genetic organization, polymorphism. and function of the bovine major histocompatibility Complex Region of Domestic Animal Species. CRC Press, Boca Raton. FL
  10. Millerr, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extraction DNA from human nucleated cells. Nucleic Acids Res. 16:1215 https://doi.org/10.1093/nar/16.3.1215
  11. Sharif, S., B. A. Mallard, B. N. Wilkie, J. M. Sargeant, H. M. Scott, J. C. M. Dekkers and K. E. Leslie. 1998a. Associations of the bovine major histocompatibility complex DRB3 alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle. Anim. Genet. 29:185-193 https://doi.org/10.1111/j.1365-2052.1998.00318.x
  12. Sharif, S., B. A. Mallard, B. N. Wilkie, J. M. Sargeant, H. M. Scott, J. C. M. Dekkers and K. E. Leslie. 1998b. Associations of the bovine major histocompatibility complex DRB3 with production traits in Canadian dairy cattle. Anim. Genet. 30:157-160 https://doi.org/10.1046/j.1365-2052.1999.00459.x
  13. Sharif, S., B. A. Mallard and J. M. Sargeant. 2000. Presence of glutamine at position 74 of pocket 4 in the BoLA-DR antigen binding groove is associated with occurrence of clinical mastitis caused by Staphylococcus species. Vet. Immunol. Immunopathol. 76:231-238 https://doi.org/10.1016/S0165-2427(00)00216-6
  14. Van Dorp, R. T., S. W. Martin, M. M. Shoukri, J. P. Noordhuizen and J. C. Dekkers. 1999. An epidemiologic study of disease in 32 registered Holstein dairy herds in British Columbia. Canad. J. Vet. Res. 63:185-192
  15. Van Eijk, M. J. T., J. A. Stewart-Haynes and H. A. Lewin. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Anim. Genet. 23:483-496 https://doi.org/10.1111/j.1365-2052.1992.tb00168.x
  16. Yoshida, T., H. Mukoyama, H. Furuta, S. Ohboshi, M. Kosugiyama and H. Tomogane. 2004. Allelic frequency of PCR-RFLP type of BoLA-DRB3 in the Japanese Holstein herds and the relation to mastitis. Proceeding of 29th International Conference on Animal Genetics. Sep. 11-16. Tokyo, Japan

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