Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of IGF-I Rich Fraction from Bovine Colostral Whey on Murine Immunity

  • Hwang, Kyung-A (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Ha, Woel-Kyu (Food Research & Development Lab., Pasteur Milk Co., Ltd.) ;
  • Yang, Hee-Jin (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Lee, Soo-Won (Faculty of Life Science and Technology, Sungkyunkwan University)
  • Received : 2005.04.11
  • Accepted : 2005.08.10
  • Published : 2006.02.01
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Abstract

Insulin-like growth factor-I (IGF-I) rich fraction, collected components between 1 kDa and 30 kDa, was fractionated from bovine colostral whey using an ultrafiltration membrane. IGF-I was confirmed in the collected IGF-I rich fraction by both SDS-PAGE and Western blotting. The concentration of IGF-I in the IGF-I rich fraction was 10 ng/mg protein. One hundred microliters of the reconstituted IGF-I rich fraction was intraperitoneally injected into ICR male mice for 2 weeks at 24 h intervals. The functions of peritoneal macrophages, including phagocytosis, interleukin (IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ production, and nitric oxide and hydrogen peroxide production, were enhanced significantly by the administration of the IGF-I rich fraction in a dose-dependent manner (p<0.01). The proliferation of Concanavalin (Con) A-stimulated and Lipopolysaccharide (LPS)-stimulated splenocytes was also determined to have been enhanced significantly by the administration of the IGF-I rich fraction in a dose-dependent manner (p<0.01). Our results indicate that the administration of IGF-I rich fraction obtained from bovine colostral whey enhances both innate and acquired immunity for ICR male mice.

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References

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