Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Isolation of Caenorhabditis elegans Mutants Defective in Chemotaxis toward cAMP

  • Jeong, Jin-A (School of Life Sciences, Chungbuk National University) ;
  • Cho, Nam-Jeong (School of Life Sciences, Chungbuk National University)
  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Chemotactic behavior is essential for the survival of animals. However, the mechanism by which animals carry out chemotaxis is poorly understood. To explore the biochemical events underlying chemotaxis, we isolated C. elegans mutants that displayed abnormal chemotactic responses to cAMP, a strong attractant for C. elegans. Based on their responses to other chemoattractants, the mutant animals could be classified into five groups: (1) animals with defective chemotaxis to cAMP only; (2) animals with defective chemotaxis to both cAMP and cGMP; (3) animals with defective chemotaxis to water-soluble attractants; (4) animals with defective chemotaxis to both water-soluble and volatile attractants; and (5) animals with enhanced chemotactic responses. We expect that analyses of these mutants will help understand the molecular mechanisms underlying chemotaxis in C. elegans.

Keywords

References

  1. Bargmann CI and Horvitz HR (1991) Chemosensory neurons with overlapping functions direct chemotaxis to multiple chemicals in C. elegans. Neuron 7: 729-742 https://doi.org/10.1016/0896-6273(91)90276-6
  2. Bargmann CI (1993) Genetic and cellular analysis of behavior in C. elegans. Annu Rev Neurosci 16: 47-71 https://doi.org/10.1146/annurev.ne.16.030193.000403
  3. Bargmann CI, Hartwieg E, and Horvitz HR (1993) Odorantselective genes and neurons mediate olfaction in C. elegans. Cell 74: 515-527 https://doi.org/10.1016/0092-8674(93)80053-H
  4. Bargmann CI (1998) Neurobiology of the Caenorhabditis elegans genome. Science 282: 2028-2033 https://doi.org/10.1126/science.282.5396.2028
  5. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71-94
  6. Chalfie M, Tu Y, Euskirchen G; Ward WW, and Pmsher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805 https://doi.org/10.1126/science.8303295
  7. Colbert HA and Bargmann CI (1995) Odorant-specific adaptation pathways generate olfactory plasticity in C. elegans. Neuron 14: 803-812 https://doi.org/10.1016/0896-6273(95)90224-4
  8. Dusenbery DB, Sheridan RE, and Russell RL (1975) Chemotaxis-defective mutants of the nematode Caenorhabditis elegans. Genetics 80: 297-309
  9. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, and Mello CC (1998) Potent and specific genetic interference by double-stmnded RNA in Caenorhabditis elegans. Nature 391: 806-811 https://doi.org/10.1038/35888
  10. Jeong J, Hwang JM, and Cho NJ (1996) Chemosensation in Caenorhabditis elegans: identification of attractants and analysis of chemotactic behavior. Genet Eng Res 10: 1-15
  11. Matsuki M, Kunitomo H, and lino Y (2006) Goa regulates olfactory adaptation by antagonizing Gqa-DAG signaling in Caenorhabditis elegans. Proc Natl Acad USA 103: 1112-1117 https://doi.org/10.1073/pnas.0506954103
  12. Mori I and Ohshima Y (1997) Molecular neurogenetics of chemotaxis and thermotaxis in the nematode Caenorhabditis elegans. BioEssays 19: 1055-1064 https://doi.org/10.1002/bies.950191204
  13. Sengupta P, Chou JH, and Bargmann CI (1996) odr-10 encodes a seven tmnsmembrane domain olfactory receptor required for responses to the odorant diacetyl. Cell 84: 899-909 https://doi.org/10.1016/S0092-8674(00)81068-5
  14. Strmecki L, Greene DM, and Pears CJ (2005) Developmental decisions in Dictyostelium discoideum. Dev Bioi 284: 25-36 https://doi.org/10.1016/j.ydbio.2005.05.011
  15. Troemel ER, Chou JH, Dwyer ND, Colbert HA, and Bargmann CI (1995) Divergent seven transmembrane receptors are candidate chemosensory receptors in C. elegans. Cell 83 : 207-218 https://doi.org/10.1016/0092-8674(95)90162-0
  16. Troemel ER, Kimmel BE, and Bargmann CI (1997) Reprogmmming chemotaxis responses: sensory neurons defme olfactory preferences in C. elegans. Cell 91: 161-169 https://doi.org/10.1016/S0092-8674(00)80399-2
  17. Ward S (1973) Chemotaxis by the nematode Caenorhabditis elegans: identification of attractants and analysis of the response by use of mutants. Proc Natl A cad USA 70: 817-821 https://doi.org/10.1073/pnas.70.3.817
  18. White JG; Southgate E, Thomson IN, and Brenner S (1986) The structure of the nervous system of the nematode Caenorhabditis elegans. Phil Trans R Soc Lond B 314: 1-340 https://doi.org/10.1098/rstb.1986.0056