Prevalence of Antibiotics in Nectar and Honey in South Tamilnadu, India

  • Solomon, RD. Jebakumar (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University) ;
  • Santhi, V. Satheeja (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University) ;
  • Jayaraj, Vimalan (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University)
  • 발행 : 2006.09.30

초록

Reverse-Phase High-performance Liquid Chromatography (RP-HPLC) based technique is one of the most sensitive techniques to detect the antibiotics present in honey. In the southern part of Tamilnadu, India, majority of the farmlands are occupied by plantations such as coconut, banana and rubber. A variety of antimicrobial compounds and antibiotics, which have been reported in pollen, nectar and other floral parts of the plant, gets accumulated in honey through honeybees (Apis mellifera). We have collected the nectar samples from banana (Musa paridasiaca) and rubber (Ficus elastica) flowers and the honey from honey hives of banana and rubber cultivated areas. The extracted nectar and honey samples are subjected to RP-HPLC analysis with authentic antibiotic standards. Nectar and honey samples showed 4-17, 11-29 ${\mu}g/kg$ of streptomycin, 2-29, 3-44 ${\mu}g/kg$ of ampicillin and 17-34, 26-48 ${\mu}g/kg$ of kanamycin respectively.

키워드

참고문헌

  1. AI-Jabri AA (2005) Honey, milk and antibiotics. Afr J Biotechnol 4: 1580-1587
  2. Allen KL, Molan PC and Reid GM (1991) A survey of the antibacterial activity of some New Zealand honeys. J Pharmacy Pharmacol 43: 817-822 https://doi.org/10.1111/j.2042-7158.1991.tb03186.x
  3. Bogdanov S (2003) Current status of analytical methods for the detection of residues in bee products. APIACTA 38: 190-197
  4. Bogdanov S and Fluri P (2000) Honigqualitat und Antibiotikaruckstande, Schweiz. Bienen-Zeitung 123: 407-410
  5. Bruijnsvoort MV, Ottink SJ, Jonker KM and De Boer E (2004) Determination of streptomycin and dihydrostreptomycin in milk and honey by liquid chromatography with tandem mass spectrometry. J Chroma A 1058: 137-142 https://doi.org/10.1016/j.chroma.2004.07.101
  6. Dharmananda S (2003) Traces of chloramphenicol in Chinese Bee Products: Origin, Development, and Resolution. Institute for Traditional Medicine, Portland, Oregon, (http://www.itmonline.org/arts/bees.htm.)
  7. Efem SE, Udoh KT and Iwara CI (1992) The antibacterial spectrum of honey and its clinical significance. Infection 20: 227-229 https://doi.org/10.1007/BF02033065
  8. Heering W, Usleber E, Dietrich R and Martlbauer E (1998) Immunochemical screening for antimicrobial drug residues in commercial honey. Analyst 123: 2759-2762 https://doi.org/10.1039/a805047c
  9. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with the Folin-phenol reagents. J Biol Chem 193: 265-275
  10. Molan PC (1999) The role of honey in the management of wounds. J Wound Care 8:423-426 https://doi.org/10.12968/jowc.1999.8.8.25904
  11. Molan PC (1999) Why honey is effective as a medicine. Its use in modern medicine. Bee World 80: 80-92 https://doi.org/10.1080/0005772X.1999.11099430
  12. Molan PC (2000) Establishing honey as a recognized medicine. J Amer Apitherapy Soc 7: 1
  13. Mundo MA, Padilla-Zakour OI and Worobo RW (2004) Growth inhibition of food borne pathogens and food spoilage organisms by select raw honeys. Int J Food Microbiol 97: 1-8 https://doi.org/10.1016/j.ijfoodmicro.2004.03.025
  14. Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153: 375-380
  15. Radwan S, EI-Essawy A and Sarhan MM (1984) Experimental evidence for the occurrence in honey of specific substances active against microorganisms. Zentral Mikrobiol 139: 249-255
  16. Snowdon JA and Cliver DO (1996) Microorganisms in honey. Int J Food Microbiol 31: 1-26 https://doi.org/10.1016/0168-1605(96)00970-1
  17. Spivak M (2000) Preventative antibiotic treatments for honey bee colonies. Amer Bee J 140: 867-868
  18. Wellford TE, Eadie T and Llewellyn GC (1978) Evaluation of inhibitory action of honey on fungal growth, sporulation and aflotoxin production. Zeitschrift Fur Lebensmittel Untersuchungund-forschung a-food Res 166: 280-283 https://doi.org/10.1007/BF01127653