Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Developmental Changes in Digestive Enzymes Activity of Black Rockfish Sebastes inermis

볼락, Sebastes inermis 자.치어의 성장에 따른 소화효소 활성 변화

  • Gwak Woo-Seok (Division of Marine Bioscience, The Institute of Marine Industry, Gyeongsang National University) ;
  • Park Dae-Won (Gyeongsangnam-do Fisheries Resources and Research Institute)
  • 곽우석 (국립 경상대학교 해양생명과학부/해양산업연구소) ;
  • 박대원 (경상남도수산자원연구소)
  • Published : 2006.05.01
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Abstract

Black rockfish larvae and juveniles were reared for 95 days after parturition (DAP) in order to determine four enzyme activities (trypsin, pepsine-like enzyme, lipase, amylase) during ontogeny. Larvae were fed rotifers Brachionus plicatilis from 1 to 25 DAP, Artemia nauplii from 10 to 78 DAP and then gradually changed to pelleted feed from 30 DAP. Temperature was kept between $13.5{\sim}14.9^{\circ}C$. Trypsin and lipase activities were found in 2 DAP larvae ($7.0{\pm}1.5$ unit and $4.5{\pm}1.4$ unit, $mean{\pm}SD$, respectively). The evolution of both enzymes activities showed a profile marked by drastic increases between postflexion and juvenile stage. There is an increment on specific trypsin activity at 10 DAP, corresponding with the beginning of Artemia feeding. Pepsin-like enzyme activity was found at 11 DAP and increased drastically from 56 DAP, cor-responding with the initiation of juvenile stage. Amylase activity was also found at 11 DAP and maintained at a low level up to 38 DAP followed by a drastic increase from 39 DAP to 50 DAP. Considering our results of both trypsin and pepsin-like enzyme activities, it might be concluded that higher somatic growth of Sebastes inermis could be possible with the initiation juvenile of stage and the early juvenile stage is a suit-able period for feeding an artificial diet for fish.

본 실험은 볼락 종묘를 안정적으로 대량 생산하는 데 필 수적인 자치어기의 성장과 소화효소인 trypsin, pepsin-like enzyme, amylase 그리고 lipase의 activity를 측정하여 소화생리에 대한 기초 자료를 제공하고자 수행하였다. 산출 직후 자어의 체장은 $5.01{\pm}0.22 mm$이었던 것이 산출 후 100일에는 $36.01{\pm}1.22 mm$ 까지 성장하였고, 습중량은 산출 후 70일 전후로 급격히 증가하였다. trysin 활성은 산출후 2일째 처음으로 확인되어 $7.00{\pm}1.48$ unit을 나타내고 산출후 66일 $1425{\pm}230$ unit으로 peak를 나타내었다. pepsin-like enzyme 활성은 산출 후 11일째 처음으로 확인되었고 산출후 66일째 $902{\pm}160$ unit까지 급격히 증가하였다. Lipase 활성은 산출후 2일째 $4.5{\pm}1.4$ unit으로 확인되었고 산출후 47일계에는 $38.3{\pm}0.4$unit로 peak를 나타내었다. Amylase 활성은 산출후 11일째 $0.18{\pm}0.11$ unit으로 처음 확인되었고 산출후 50일째 $23.48{\pm}5.11$ unit까지 급격히 증가하였다. specific activity는 trypsin이 산출후 14일째, pepsin-like enzyme이 61일째 peak를 나타냈다. 볼락의 체중이 현저하게 증가하는 시기에 맞춰서 각 효소와 전활성이 급격히 상승하는 것을 확인 할 수 있었다. 이것은 이시기가 위선이 기능적으로 역할을 하는 시기와 일치하므로 단백질 소화능력의 비약적인 증가가 체중 증가에 크게 관여하였음을 나타낸다. 그러므로 본 실험에서 확인 된 체중이 급격히 증가하는 시기는 볼락 양식에 있어서 사료 전환기 즉, 생물사료에서 배합사료로 전환하기에 적당하다고 할 수 있겠다.

Keywords

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