The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Effect of temperature on the Concentration of Chrysanthemum stunt viroid in CSVd-infected Chrysanthemum

  • Chung, Bong-Nam (National Horticultural Research institute, Rural Development Administration) ;
  • Huh, Eun-Joo (National Horticultural Research institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (National Horticultural Research institute, Rural Development Administration)
  • Published : 2006.06.01
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Abstract

CSVd-infected chrysanthemum plants grown under $10^{\circ}C\;or\;15^{\circ}C$ growth chamber for 2 months resulted a higher dilution endpoint of template RNA for Reverse transcription and polymerase chain reaction (RT-PCR) than those grown for 1 month: $10^{-4}(1.35{\times}10^{-2}ug/ml)$ for 1 month, and $10^{-3}(1.35{\times}10^{-1}ug/ml)$ for 2 months. Independent experiment, shoots cut from CSVd (Chrysanthemum stunt viroid)-in footed chrysanthemum plants grown under $10^{\circ}C\;or\;20^{\circ}C$ growth chamber for 2 months showed the same CSVd concentration as control($30^{\circ}C$) at 8 weeks after moving them to normal green-house condition($30^{\circ}C$). From those results, it was concluded that even though the concentration of CSVd was reduced in plants grown at low temperatures, when they were moved to normal glass-house temperature CSVd concentration increased to that of untreated plants in 8 weeks. This conclusion was supported by the rapid replication of CSVd in chrysanthemum after infection.

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References

  1. Bachelier, J. C., Monsion, M. and Dunez, J.1976. Possibilities of improving detection of chrysanthemum stunt and obtention of viroid free plants by meristem tip culture. Acta Hart. 59:63-70
  2. Chung, B. N. Lim, J. H. Choi, S. Y., Kim, J. S. and Lee, E. J. 2005. Occurrence of Chrysanthemum stunt viroid in chrysanthemum in Korea. Plant Pathal. J. 21:377-382 https://doi.org/10.5423/PPJ.2005.21.4.377
  3. Cichal, E. P. and Kryczynski, S. 1987. A low temperature therapy and meristem-tip culture for eliminating four viroids from infected plants. J. Phytopathol. 118:341-346 https://doi.org/10.1111/j.1439-0434.1987.tb00465.x
  4. Dusi, A. N., Fonseca, M. E. N. and Avila, A. C. 1990. Occurrence of a viroid in chrysanthemum in Brazil. Plant Pathol. 36:363-637
  5. Kassanis, B. 1954. Heat therapy of virus infected plants. Ann. Appl. Biol. 41:470-474 https://doi.org/10.1111/j.1744-7348.1954.tb01146.x
  6. Kusunoki, M., Matsumoto, Y., Nakanishi, M., Soichi, N. and Sogou, K. 1993. Occurrence of chrysanthemum stunt disease in Kagawa Prefecture. Kagawaken Agricultural Experiment Station Research Report 44:19-25
  7. Mlihlbach, H. P. and Sanger, H. L. 1977. Multiplication of cucumber pale fruit viroid in inoculated tomato leaf protoplasts. J. Gen. Viral. 35:377-386 https://doi.org/10.1099/0022-1317-35-2-377
  8. Nyland, G. and Goheen, A. C. 1969. Heat therapy of virus diseases of perennial plants. Annu. Rev. Phytopath. 7:331-354 https://doi.org/10.1146/annurev.py.07.090169.001555
  9. Paludan, N. 1985. Inactivation of viroids in chrysanthemum by low temperature treatment and meristem tip culture. Acta Hort. 164:181-186
  10. Shiwaku, K., iwai, T. and Yamamoto, Y. 1996. Cloning and nucleotide sequence of Chrysanthemum stunt viroid. Hyogo Pre. Agric. Inst. (Agriculture), 44:1-4

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