Journal of Ecology and Environment

한국생태학회 (The Ecological Society of Korea)
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Identification of Ectomycorrhizal Fungi from Pinus densiflora Seedlings at an Abandoned Coal Mining Spoils

  • Park, Sang-Hyeon (Department of Biology Education, Korea National University of Education) ;
  • Jeong, Hyeon-Suk (Department of Biology Education, Korea National University of Education) ;
  • Lee, Yoo-Mee (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education) ;
  • Lee, Chang-Seok (Faculty of Environment and Life Sciences, Seoul Women's Univeristy)
  • 발행 : 2006.04.01
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초록

This study was conducted to identify native ectomycorrhizal (ECM) fungi colonizing Pinus densiflora for revegetation of abandoned coal mines in Korea. Seedlings of P. densiflora growing on coal mining spoils of a study site in Samcheok were collected. ECM roots were observed under stereomicroscope and their DNA were extracted from each root tip for a seedling for molecular identification. A PCR primer pair specific to fungi, ITS1F and ITS4, was used to amplify fungal DNA. Restriction enzymes, Alul and Hinfl were used for restriction fragment length polymorphism (RFLP). Combined with RFLP profiles and sequence analysis, total twenty one taxa were identified from the ECM root tips. Basidiomycetous fungi including Thelephoraceae, Pezizales, Laccaria, Pisolithus and Ascomycetous fungi including ericoid mycorrhizal fungi were identified from this study. Results showed that the most frequently found in the study sites was a species in Thelephoraceae. A possible use of ECM fungi identified in this study for the revegetation of abandoned coal mines with P. densiflora was discussed.

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