Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Genus- and Species-Specific Probe Design System for Pathogen Detection Based on 23S rDNA

  • Park Jun-Hyung (Busan Genome Center) ;
  • Park Hee-Kyung (Institute for Genomic Medicine, GeneIn. Co., Ltd.) ;
  • Kang Byeong-Chul (Division of Applied Bioengineering, Dongseo University) ;
  • Song Eun-Sil (Institute for Genomic Medicine, GeneIn. Co., Ltd.) ;
  • Jang Hyun-Jung (Institute for Genomic Medicine, GeneIn. Co., Ltd.) ;
  • Kim Cheol-Min (Busan Genome Center, Medical Research Institute, Department of Biochemistry, College of Medicine, Pusan National University)
  • Published : 2006.05.01
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Abstract

Amplification by universal consensus sequences in pathogenic bacterial DNA would allow rapid identification of pathogenic bacteria, and amplification of genus-specific and species-specific sequences of pathogenic bacterial DNA might be used for genotyping at the genus and species levels. For design of probes for molecular diagnostics, several tools are available as stand-alone programs or as Web application. However, since most programs can design only a few probe sets at one time, they are not suitable for large-scale and automatic probes design. Therefore, for high-throughput design of specific probes in diagnostic array development, an automated design tool is necessary. Thus, we developed a Web-based automatic system for design of genus-specific and species-specific probes for pathogen detection. The system is available at http://www.miprobe.com.

Keywords

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