폴리머 (Polymer(Korea))

  • 제30권3호
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  • Pages.259-265
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  • 2006
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  • 0379-153X(pISSN)
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  • 2234-8077(eISSN)
한국고분자학회 (The Polymer Society of Korea)
권호 표지

창상드레싱을 위한 Poly(vinyl alcohol)을 코팅한 소장점막하조직 시트의 제조와 특성 평가

Preparation and Characterization of Small Intestinal Submucosa Coated with Poly(vinyl alcohol) for Wound Dressing

  • 이민숙 (전북대학교 BK21고분자 BIN융합연구팀) ;
  • 양재찬 (전북대학교 유기신물질공학과) ;
  • 김순희 (전북대학교 유기신물질공학과) ;
  • 송인범 (전북대학교 유기신물질공학과) ;
  • 김문석 (한국화학연구원 나노생체재료연구팀) ;
  • 강길선 (전북대학교 BK21고분자 BIN융합연구팀) ;
  • 이해방 (한국화학연구원 나노생체재료연구팀)
  • Lee Min-Suk (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University) ;
  • Yang Jea-Chan (Department of Advanced Organic Materials Engineering, Chonbuk National University) ;
  • Kim Soon-Hee (Department of Advanced Organic Materials Engineering, Chonbuk National University) ;
  • Song In-Bum (Department of Advanced Organic Materials Engineering, Chonbuk National University) ;
  • Kim Moon-Suk (Medicinal Science Division, Korea Research Institute of Chemical Technology) ;
  • Khang Gil-Son (BK21 Polymer BIN Fusion Res. Team, Chonbuk National University) ;
  • Lee Hai-Bang (Medicinal Science Division, Korea Research Institute of Chemical Technology)
  • 발행 : 2006.05.01
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초록

손상된 조직의 재생을 촉진시키는 여러가지 성장인자를 함유한 소장점막하조직(small intestinal subucosa, SIS)과 드레싱 교체시 손상된 피부로부터 박리가 용이한 수화젤 특성을 갖는 PVA를 이용하여 창상 치유를 촉진시키고 박리가 용이한 드레싱을 제조하였다. SIS 시트를 2, 4 및 10 wt%로 제조한 PVA 용액에 침지시킨 후 각기 다른 몰드에서 동결건조함으로써 PVA가 코팅된 SIS 시트를 제조하였다. 제조한 시트는 인장시험을 통해 기본 물성을 평가하였고, 분해도, 물 흡수성, 그리고 in vitro 실험을 통해 그 특성을 조사하였다. PVA를 코팅한 SIS(PVA-SIS) 시트의 인장강도와 신장률은 PVA의 함량이 증가함에 따라 각각 감소, 증가하였으며, 분해도는 본래의 SIS에 비해 감소하였고, SIS에 2 및 4 wt%의 PVA가 코팅되었을 때 물 흡수성이 증가하였다. 또한 전 PVA-SIS 시트에서 세포부착도가 본래의 SIS 시트에 비해 낮음을 확인하였다. 이로써 PVA를 코팅한 SIS 시트가 창상 치료를 위한 드레싱제와 생분해성 이식 제제로서 사용가능하리라 판단되었다.

Small intestinal submucosa (SIS) consists of some growth factors which can stimulate cell activity, and PVA has been widely utilized in the area of wound dressing as hydrogel which is easy to be removed from wounds. In this study, native SIS sheets were coated with PVA by immersing them into 2, 4, and 10 wt% of PVA solution and then lyophilized on two type of molds to endow the prepared wound dressing with easy removal property from wounds. The mechanical properties were examined through tensile test. Moreover, enzymatic degradation, water uptake, and in vitro test were carried out to characterize the prepared SIS sheets. The tensile strength of the SIS sheets coated with PVA (PVA-SIS) were decreased, whereas the elongation were increased. Degradation ratio of the PVA-SIS sheets was decreased compare to native SIS. Water uptake ability was improved at 2 and 4 wt% of PVA. The degree of fibroblast attachment was lower than the native SIS sheets. In conclusion, this study suggests that the PVA coated SIS sheets have a potential for the applications of wound dressing and biodegradable injectable materials.

키워드

참고문헌

  1. M. Carenza, Radiation Phys. Chem., 39, 485 (1992)
  2. M. G. Cascone, N. Barbani, C. Cristallini, P. Giusti, G. Ciardelli, and L. Lazzeri, J. Biomater. Sci. Polym. Ed., 12, 267 (2001) https://doi.org/10.1163/156856201750180807
  3. V. Crescenzi, G. Paradossi, P. Desideri, M. Dentini, F. Cabalieri, E. Amici, and R. Lisi, Polymer Gels Network, 5, 225 (1997) https://doi.org/10.1016/S0966-7822(96)00051-2
  4. D. C. Harxh and S. H. Gehrake, J. Control. Release, 17, 175 (1991) https://doi.org/10.1016/0168-3659(91)90057-K
  5. N. A. Peppas and J. E. Scott, J. Control. Release, 18, 95 (1992) https://doi.org/10.1016/0168-3659(92)90178-T
  6. O. Z. Higa, S. O. Rogero, L. D. B. Machado, M. B. Mathor. and A. B. Lugao, Radiation Phys. Chem., 55, 705 (1999) https://doi.org/10.1016/S0969-806X(99)00215-7
  7. M. Zhai, F. Yoshii, T. Kume, and K. Hashim, Carbohyd. Polymers, 50, 295 (2002) https://doi.org/10.1016/S0144-8617(02)00031-0
  8. L. Zhao, H. Mitomo, M. Zhai, F. Yoshii, N. Nagasawa, and T. Kume, Carbohyd. Polymers, 53, 439 (2003) https://doi.org/10.1016/S0144-8617(03)00103-6
  9. M. Rosen, J. Ponsky, R. Petras, A. Fanning, F. Brody, and F. Duperier, Surgery, 132, 480 (2002) https://doi.org/10.1067/msy.2002.126505
  10. J. P. Hodde, Tissue Eng., 8, 295 (2002) https://doi.org/10.1089/107632702753725058
  11. J. P. Hodde, S. F. Badylak, A. O. Brightman, and S. L. Voytik-Harbin, Tissue Eng., 2, 209 (1996) https://doi.org/10.1089/ten.1996.2.209
  12. T. B. McPherson and S. F. Badylak, Tissue Eng., 4, 75 (1998) https://doi.org/10.1089/ten.1998.4.75
  13. M. J. Shon, S. Y. Lee, T. H. Kim, C. K. You, S. Kim, P. H. Choung, Y. Son, E. K. Park, and S. Y. Kim, Tissue Eng. Regen. Med., 2, 356 (2005)
  14. S. Y. Lee, M. J. Shon, T. H. Kim, S. Kim, Y. Son, S. Y. Kim, and E. K. Park, Tissue Eng. Regen. Med., 2, 362 (2005)
  15. J. P. Hodde, and M. C. Hiles, Wounds, 13, 195 (2001)
  16. J. P. Hodde, DMJ. Ernst, and M. C. Hiles, J. Wound Care, 14, 23 (2005) https://doi.org/10.12968/jowc.2005.14.1.26721
  17. C. A. McDevitt, G. M. Wildey, and R. M. Cutrone, J. Biomed. Mater. Res., 67A, 637 (2003) https://doi.org/10.1002/jbm.a.10144
  18. G. Khang, M. S. Kim, S. H. Cho, I. Lee, J. M. Rhee, and H. B. Lee, Tissue Eng. Regen. Med., 1, 9 (2004)
  19. M. S. Kim, K. D. Hong, H. W. Shin, S. H. Kim, S. H. Kim, M. S. Lee, W. Y. Jang, S. Khang, and H. B. Lee, Intern. J. Biological Macromolecules, 36, 54 (2005) https://doi.org/10.1016/j.ijbiomac.2005.03.013
  20. W. H. Park and J. K. Lee, Polymer(Korea), 21, 46 (1997)
  21. F. L. Martien, Encyclopedia of Polym. Sci. Eng., New York, Wiley, vol.17, p.167 (1986)
  22. B. L. Seal, T. C. Otero, and A. Panitch, Mater. Sci. Eng., 34, 147 (2001) https://doi.org/10.1016/S0927-796X(01)00035-3
  23. M. B. Huglin and M. B. Zakaria, J. Appl. Polym. Sci., 31, 457 (1986) https://doi.org/10.1002/app.1986.070310213
  24. F. Yishii, Y. Zhashan, K. Isobe, K. Shinozaki, and K. Makuchi, J. Membrane Sci., 109, 257 (1999) https://doi.org/10.1016/0376-7388(95)00196-4
  25. K. J. Cho, D. K. Song, S. H. Oh, Y. J. Koh, S. H. Lee, M. C. Lee, and J. H. Lee, Tissue Eng. Regen. Med., 2, 115 (2005)
  26. G. Khang, P. K. Shin, I. Y. Kim, B. Lee, S. J. Lee, Y. M. Lee, H. B. Lee, and I. W. Lee, Macromol. Res., 10, 158 (2002) https://doi.org/10.1007/BF03218266
  27. K. M. Clark, G. C. Lantz, S. K. Salibury, S. F. Badylak, M. C. Hiles, and S. L. Voytik, J. Surg. Res., 60, 107 (1996) https://doi.org/10.1006/jsre.1996.0018
  28. U. T. Shettigar, R. Jogannathan, and R. Natarajan, Artif. Organs, 6, 256 (1982) https://doi.org/10.1111/j.1525-1594.1982.tb01670.x
  29. S. K. Han and S. J. Choi, 'The Demand of Dressing', in Advance in Wound Care, H. J. Yoo, J. H. Kim, M. H. Park K. K. Lee, J. H. Lee, Y. S. Yoo, S. K. Han, and G. Yushimize, Editors, Korea Medical Book Publisher Co., Seoul, pp 170 (2003)
  30. M. Rosen, J. Ponsky, R. Petras. A. Fanning, F. Brody, and F. Duperier. Surgery, 132, 480 (2002) https://doi.org/10.1067/msy.2002.126505
  31. R. H. Schmedlen, K. S. Kristyn, and J. L. Jennifer, Biomaterials, 23, 4325 (2002) https://doi.org/10.1016/S0142-9612(02)00177-1