Archives of Pharmacal Research

  • 제29권5호
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  • Pages.400-404
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  • 2006
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  • 0253-6269(pISSN)
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  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Electrically Stimulated Relaxation is not Mediated by GABA in Cat Lower Esophageal Sphincter Muscle

  • Park Sun-Young (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Shin Chang-Yell (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Song Hyun-Ju (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Min Young-Sil (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • La Hyen-O (Department of Pharmacology, College of Medicine, The Catholic University of Korea) ;
  • Lee Jun-Woo (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Kim Do-Young (Department of Pharmacology, College of Pharmacy, Chung Ang University) ;
  • Je Hyun-Dong (Department of Pharmacology, College of Pharmacy, Catholic University of Daegu) ;
  • Sohn Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung Ang University)
  • 발행 : 2006.05.01
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초록

This study examined the effect of Gamma-Amino butyric acid (GABA) and selective GABA receptor related drugs on the electrically stimulated relaxation in the lower esophageal sphincter muscle (LES) of a cat. Tetrodotoxin $(10^{-6}\;M)$ suppressed the electrically stimulated (0.5-5 Hz) relaxation of the LES. However, guanethidine $(10^{-6}\;M)$ and atropine $(10^{-6}\;M)$ had no effect indicating that the relaxations were neurally mediated via the nonadrenergic and noncholinergic (NANC) pathways. NG-nitro-L-arginine methyl ester ($10^{-4}M$, L-NAME) also inhibited the relaxant response but did not completely abolish the electrically stimulated relaxation with 60% inhibition, which suggests the involvement of nitric oxide as an inhibitory transmitter. This study examined the role of GABA, an inhibitory neurotransmitter, on neurally mediated LES relaxation. GABA ($10^{-3}-10^{-5}M$, non selective receptor agonist), muscimol ($10^{-3}-10^{-5}M$, GABA-A agonist), and baclofen ($10^{-3}-10^{-5}M$, GABA-B agonist) had no significant effect on the electrically stimulated relaxation. Moreover, bicuculline ($10^{-5}M$, GABA-A antagonist) and phaclofen ($10^{-5}M$, GABA-B antagonist) had no inhibitory effect on the electrically stimulated relaxation. This suggests that GABA and the GABA receptor are not involved in the electrically stimulated NANC relaxation in the cat LES.

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참고문헌

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