한국전통의학지 (The Journal of Traditional Korean Medicine)

한국전통의학연구소 (Korea Bio Medical Science Institute)
권호 표지

폐암세포에 대한 부자(附子) 추출물의 독성 효과

Cytotoxic Effects of Radix Aconiti Extract in Lung Cancer Cell Lines

  • 권강범 (원광대학교 한의과대학 한방생리학교실) ;
  • 김은경 (원광대학교 한의과대학 한방생리학교실) ;
  • 문형철 (원광대학교 한의과대학 침구학교실) ;
  • 송용선 (원광대학교 한의과대학 재활의학교실) ;
  • 류도곤 (원광대학교 한의과대학 한방생리학교실)
  • Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Eun-Kyung (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Moon, Hyung-Cheal (Acupuncture & Moxibustion, College of Oriental Medicine, Wonkwang University) ;
  • Song, Yung-Sun (Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Ryu, Do-Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2006.12.29
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초록

The aim of this study was to investigate the cytotoxic effect and its mechanism on Radix Aconiti(RA) extract in lung cancer cell lines. RA extract treatment decreased the cell viability in a dose-dependent fashions in lung cancer cells including A549, H460, H23 and H157 cells. Many investigators reported that A549 and H460 cells expressed wild-type p53, but H23 and H157 cells preserved mutated p53. After treatment with RA extract in A549 and H460 cells, we measured the expression of p53 protein levels using Western blot. analysis. In both cells treated with RA extracts, p53 protein expressions were increased in a dose-dependent manner. In our experiments, RA extracts also have cytotoxic effects in H23 and H157, which have mutated p53. Treatment with RA extract decreased bcl-2 protein expressions in both cells. These results suggest that RA extracts have cytotoxic effects via p53 expression increase and bcl-2 inhibitable pathways in A549, H460 cells and H23, H157 cells, respectively.

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