Journal of Nutrition and Health

  • 제39권4호
  • /
  • Pages.338-346
  • /
  • 2006
  • /
  • 2288-3886(pISSN)
  • /
  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지

이소플라본 섭취수준이 성장기 암컷 쥐의 골밀도에 미치는 영향

The Effects of Level of Isoflavones Supplementation on Bone Mineral Density in Growing Female Rats

  • 최미자 (계명대학교 식품영양학과) ;
  • 정윤정 (계명대학교 식품영양학과)
  • Choi Mi-Ja (Department of Food and Nutrition, Keimyung University) ;
  • Jung Yun-Jung (Department of Food and Nutrition, Keimyung University)
  • 발행 : 2006.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The overall purpose of this study was to investigate the effects of level of isoflavones supplementation on bone metabolism in growing female rats. The effects of level of isoflavones supplementation on bone mineral density (BMD) and bone mineral content (BMC) were inspected in this study. Forty-five rats divided into three groups: Casein, $^1/{_2}IF$, IF. The serum and urine concentrations of calcium and phosphorus were determined. BMD and BMC were estimated by using PIXImus (GE Lunar Co, Wisconsin.) in spine and femur on 3, 6, 9 weeks after feeding. This study of results were as follows: The isoflavones supplementation level did not affect weight gain, mean food intake and food efficiency ratio. The serum concentration of calcium, phosphorus were not significantly different by different level of isoflavones supplementation. The urinary calcium and phosphorus excretion were not significantly different, too. Spine and femur BMD, BMC were not significantly increased by different level of isoflavones supplementation on 3 and 6 weeks after feeding. Spine BMD and spine BMC per weight, femur BMC per weight were significantly increased in the groups $^1/{_2}IF$ and IF at the ninth week after feeding, but there was no significant difference by different level of isoflavones supplementation. Spine BMD per weight and femur BMD per weight were significantly higher in the group of IF than in the group of Casein and $^1/{_2}IF$ at the ninth week after feeding. These results suggest that the group of IF with rich isoflavones supplementation was effective to the increase of BMD spine and femur in growing female rats, respectively.

키워드

참고문헌

  1. NIH Consensus Conference. Osteoporosis prevention, diagnosis and therapy. JAMA 285: 785-795, 2001 https://doi.org/10.1001/jama.285.6.785
  2. Messina M. Soyfood, soybean isoflavones and bone health. Korean Soybean Digest 15: 122-136, 1998
  3. Arjmandi BH, Birnbaum R, Goyal NV, Getlinger MJ, Juma S, Alekel L, Hasler CM, Drum ML, Hollis BW, Kukreja SC. Bonesparing effect of soy protein in ovarian hormone-deficient rats is related to its isoflavone content. Am J Clin Nutr 68(suppl): 1364s-1368s, 1998
  4. Omi N, Aoi S, Murata K, Wzawa I. Evaluation of the effect of soybean milk and soybean milk peptides on bone metabolism in the rat model with ovariectomized osteoporosis. J Nutr Sci Vitaminol 40: 201-211, 1994 https://doi.org/10.3177/jnsv.40.201
  5. Arjmandi BH, Alekel L, Hollis BW. Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis. J Nutr 126: 161-167, 1996 https://doi.org/10.1093/jn/126.1.161
  6. Arjmandi BH, Birnbaum R, Goyal NV. Bone-sparing effect of soy protein in ovarian hormone-deficient rats is related to its isoflavone content. Am J Clin Nutr 68: 1364-1368, 1998 https://doi.org/10.1093/ajcn/68.6.1364S
  7. Fanti P, Monier-Faugere MC, Geng Z. The phytoestrogen genistein reduces bone loss in short-term ovariectomized rats. Osteoporosis Int 8: 274-281, 1998 https://doi.org/10.1007/s001980050065
  8. Ishida H, Uesugi T, Hirai K. Preventive effects of the plant isoflavones, daidzin and genistin, on bone loss in ovariectomized rats fed a calcium-deficient diet. Biol Pharm Bull 21: 62-66, 1998 https://doi.org/10.1248/bpb.21.62
  9. Horiuchi T, Onouchi T, Takahashi M, Ito H, Orimo H. Effect of soy protein on bone metabolism in postmenopausal women. Osteoporosis Int 11: 721-724, 2000 https://doi.org/10.1007/s001980070072
  10. Atkinson C, Compston JE, Day NE, Dowsett M, Bingham SA. The effects of phytoestrogen isoflavones on bone density in women: A double-blind, randomized, placebo-controlled trial. Am J Clin Nutr 79: 326-333, 2004 https://doi.org/10.1093/ajcn/79.2.326
  11. Chen YM, Ho SC, Lam SSH, Ho SSS, Woo SLF. Soy isoflavones have a favorable effect on bone loss in Chinese postmenopausal women with lower bone mass: A double-blind, randomized, controlled trial. J Clin Endocrinol Metab 88(10): 4740- 4747, 2003 https://doi.org/10.1210/jc.2003-030290
  12. Picherit C, Chanteranne B, Pelissero CB, Davicco MJ, Lebecque P, Barlet JP, Coxam V. Does-dependent bone-sparing effects of dietary isoflavones in the ovariectomized rats. British Journal of Nutrition 85: 307-316, 2001 https://doi.org/10.1079/BJN2000252
  13. Picherit C, Bennetau-Pelissero C, Chanternne B, Lebecque P, Cavicco MJ, Barlet JP, Coxam V. Soybean isoflavones dosedependently reduce bone turnover but do not reverse established osteopenia in adult ovariectomized rats. J Nutr 131: 723-728, 2001 https://doi.org/10.1093/jn/131.3.723
  14. Kardinaal AF, Morton MS, Bruggermann-Rotgans IE, Beresteijn EC. Phyto-oestrogen excretion and rate of bone loss in postmenopausal women. European Journal of Clinical Nutrition 52 (11) 850-855, 1998 https://doi.org/10.1038/sj.ejcn.1600659
  15. Nagata C, Shimizu H, Takami R, Hayashi M, Takeda N, Yasuda K. Soy product intake and serum isoflavonoid and estradiol concentrations in relation to bone mineral density in postmenopausal Japanese women. Osteoporosis Int 13(3): 200-204, 2002 https://doi.org/10.1007/s001980200014
  16. Mei J, Yeung SSC, Kung AWC. High dietary phytoestrogen intake is associated with higher bone mineral density in postmenopausal but not premenopausal women. J Clin Endocrinol Metab 86: 5217-5221, 2001 https://doi.org/10.1210/jc.86.11.5217
  17. Somekawa Y, Chiguchi M, Ishibashi T, Aso T. Soy intake related on menopausal symptoms, serum lipids, and bone mineral density in postmenopausal Japanese women. Obstet Gynecol 97(1): 109-115, 2001 https://doi.org/10.1016/S0029-7844(00)01080-2
  18. Greendale GA, Fitzgerald G, Huang MH, Sternfeld B, Gold E, Seeman T, Sherman S, Sowers M. Dietary soy isoflavones and bone mineral density: Results from the study of women's health across the nation. Am J Epidemiol 155: 746-754, 2002 https://doi.org/10.1093/aje/155.8.746
  19. Anderson JJB, Garner SC. The effects of phytoestrogens on bone. Nutrition Research 17(10): 1617-1632, 1997 https://doi.org/10.1016/S0271-5317(97)00156-5
  20. Anderson JJ, Ambrose WW, Garner SC. Biphasic effects of genistein on bone tissue in the ovariectomized, lactating rat model. Proc Soc Exp Biol Med 217: 345-350, 1998
  21. Deyhim F, Stoecker BJ, Brusewitz GH, Arjmadi BH. The effects of estrogen depletion and isoflavone on bone metabolism in rats. Nutrition Research 23: 123-130, 2003 https://doi.org/10.1016/S0271-5317(02)00477-3
  22. Cassidy A, Bingham S, Setchell KD. Biological effects of a diet of soy protein rich in isoflavones on menstrual cycle of premenopausal women. Am J Clin Nutr 60: 333-340, 1994 https://doi.org/10.1093/ajcn/60.3.333
  23. Choi MJ. Effects of soy protein on bone mineral content and bone mineral density in growing male rats. Korean J Nutr 35 (4): 409-413, 2002
  24. Choi MJ, Chae JH. The effect of isoflavone supplementation on bone mineral density and bone mineral content in growing male rats. Korean J Nutr 38(5): 373-379, 2005
  25. Choi MJ, Jo HJ. Effects of soy protein and isoflavones on bone mineral density in growing female rat. Korean J Nutr 36(4): 59- 367, 2003
  26. Jo HJ. The effects of soy protein and soy isoflavones on bone and lipid metabolism in growing female rats. Dissertation of Ph. D., Keimyung University, Daegu, 2002
  27. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents. J Nut 123: 1939-1951, 1993 https://doi.org/10.1093/jn/123.11.1939
  28. Anderegg G, Flaschka H, Sallmann R, Schwarzenboch G. Metal indicators. Helvet Chem Acta 37: 111-120, 1954
  29. Daly JA, Ertings HG. Direct method for determine inorganic phosphate in serum with the centrifichem. Clin Chem 18: 263-265, 1972
  30. Lee YS. Introduction of experimental animal study. Charoon, pp.126-127, Seoul, 2000
  31. Uesugi T, Toda T, Tsuji K, Ishida H. Comparative study on reduction of bone loss and lipid metabolism abnormality in ovariectomized rats by soy isoflavones, daidzin, genistin, and glycitin. Biol Pharm Bull 24(4): 368-372, 2001 https://doi.org/10.1248/bpb.24.368
  32. Choi MJ. Effects of level of calcium and isoflavones on bone mineral density in growing female rats. 2003 The Korean Nutrition Society Annual meeting and symposium, Abstract, 2003
  33. Kim MS. Beneficial effect of soy isoflavone on bone loss and hyperlipidemia in ovariectomized rats. Dissertation of Ph. D., Seoul National University, Seoul, 1999
  34. Arjmandi BH, Khalil DA, Hollis BW. Soy protein: Effects on intestinal calcium transport, serum vitamin D, and insulin-like growth factor-I in ovariectomized rats. Calcif Tissue Int 70(6): 483-487, 2002 https://doi.org/10.1007/s00223-001-1100-4
  35. Murkies AL, Wilcox G, Davis SR. Phytoestrogen. J Clin Endocrinol Metab 83(2): 297-303, 1998 https://doi.org/10.1210/jc.83.2.297
  36. Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JJW. Soy protein and isoflavones: Their effects on blood lipids and bone density in postmenopausal women. Am J Clin Nutr 68 (suppl): 1375s-1379s, 1998
  37. Wu J, Wang XX, Chiba H, Higuchi M, Takasaki M, Ohta A, Ishimi Y. Combined intervention of exercise and genistein prevented androgen deficiency-induced bone loss in mice. J Appl Physiol 94: 335-342, 2003 https://doi.org/10.1152/japplphysiol.00498.2002