Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Cancer Prevention and Immune Stimulation of Fractions from Capsosiphon fulvescens

매생이 추출분획의 암 예방 및 면역증진 효과

  • Kim, Nam-Young (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Jang, Min-Kyung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Jang, Jeong-Su (Central Research Institute, Binex Co., Ltd.) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 김남영 (신라대학교 의생명과학대학 제약공학과) ;
  • 장민경 (신라대학교 의생명과학대학 제약공학과) ;
  • 이동근 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과) ;
  • 하종명 (신라대학교 의생명과학대학 제약공학과) ;
  • 하배진 (신라대학교 의생명과학대학 제약공학과) ;
  • 장정수 ((주)바이넥스 기업부설연구소) ;
  • 이상현 (신라대학교 의생명과학대학 제약공학과)
  • Published : 2006.10.25
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Abstract

The fractions of Capsosiphon fulvescens were studied to verify the anticancer and immunostimulating activity. The fractions from the ethanol extract of C. fulvescens were prepared by the systematic extraction procedure with the solvents such as hexane, ethyl ether, methanol, butanol and H$_2$O. The cytotoxic effects of C. fulvescens fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2)and rat glioma cell line (C6) were investigated. Ethyl ether fraction of C. fulvescens showed the highest cytotoxicity against all four cell lines tested. In addition, H$_2$O fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of C. fulvescens fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of C. fulvescens extract stimulated NO production with concentration dependant manner. These results suggest that C. fulvescens may be a useful candidate for a natural antitumor and immune-stimulating agent.

Keywords

References

  1. Shin, H.K. The development of functional food and research trend. Food Sci. and Industry, 30:2-13, 1997
  2. Choe, M. Dietary fats and cance. J. Korean Soc. Food Nutr., 20:513-518, 1991
  3. Murakami, A., Ohigashi, H. and Koshimizu, K. Anti-tumor promotion with food phytochemicals: a strategy for cancer chemoprevention. Biosci. Biotech. Biochem, 60:1-8l, 1996 https://doi.org/10.1271/bbb.60.1
  4. Steinmetz, K.A. and Potter, J.D. Vegetabl, fruit and cancer II mechanism. Cancer Causes Aontril., 2:427-442, 1991 https://doi.org/10.1007/BF00054304
  5. Newmark, H.L. Plant phenolics as potential cancer prevention agents. Adv. Exp. Med. Biol., 401:25-34, 1996
  6. Drageted, L.O., Strube, M. and Larsen, J.C. Cancer- protective factor in fruit and vegetable: biochemical and biological background. Pharmacol. Toxicol., 72:116-135, 1993
  7. Azuine, M.A., Goswami, U.C. and Katal, J.J. Antimutagenic and anticarcinogenic effects of carotenoids and dietary palm oil. Nutr. Cancer, 17:287-295, 1992 https://doi.org/10.1080/01635589209514198
  8. Yu, H.J. Inhibitory effect on the melanogenesis of ethanol extract of Capsosiphon fulvescens. PhD Dissertation, Wonkwang University, 2004
  9. Kang, Y.S. A comprehensive bibliography on the fishery special commodity in Korea. Suhyepmunhwasa, Seoul, p 418-421, 2000
  10. Park, J.C., Choi, J.S., Song, S.H., Choi, M.R. and Choi, J.W. Hepatoprotective effect of extracts and phenolic compound from marine algae in bromobenzene-treated rats. Korean J. Pharmacogn., 28:239-246, 1997
  11. Han, H.A. A study of flavor on Capsosiphon fulvescens. Yosu University, 2002
  12. Michael, C.A., Dominic, A.S. and Anue, M. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res., 48:589-595, 1988
  13. Carmichael, J., De, W., Graff, G., Gazder, A.F., Minna, J.D. and Mitchell, J.B. Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing. Cancer Res., 47:936-942, 1987
  14. Kim, J.H., Kim, B.S., Choi, J.J., Kim, K.M., Yoo, N.C., Choi, J.H., Lim, H.Y., Roh, J.K., Lee, K.S. and Kim, B.S. Effects of verapamil, tamoxifen and cyclosporin A for the modulation of multidrug resistance in human lung cancer cell lines. J. Kor. Cancer Assoc., 25:225-230, 1993