Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Ursolic Acid from Corni fructus on the Hydrogen Peroxide-induced Damage of HEI-OC1 Auditory Cells

산수유로부터 추출한 ursolic acid가 과산화수소로 손상된 HEI-OC1 청각세포보호에 미치는 영향

  • Yu, Hyeon-Hee (Department of Food and Nutrition, Kunsan National University) ;
  • Seo, Se-Jeong (Department of oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University, VCRC) ;
  • Hur, Jong-Moon (Department of oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University, VCRC) ;
  • Park, Rae-Kil (Department of Microbiology & Vestibulocochlear Research Center, School of Medicine, Wonkwang University, VCRC) ;
  • So, Hong-Seob (Department of Microbiology & Vestibulocochlear Research Center, School of Medicine, Wonkwang University, VCRC) ;
  • Jeon, Byung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • You, Yong-Ouk (Department of oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University, VCRC)
  • 유현희 (군산대학교 식품영양학과) ;
  • 서세정 (원광대학교 치과대학 구강생화학교실) ;
  • 허종문 (원광대학교 치과대학 구강생화학교실) ;
  • 박래길 (원광대학교 의과대학 미생물학과) ;
  • 소홍섭 (원광대학교 의과대학 미생물학과) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실)
  • Published : 2006.12.25
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Abstract

The fruits of Cornus officinalis have been used in traditional Oriental medicine for treatment of inner ear diseases, such as tinnitus and hearing loss. In the present study, we showed that the ursolic acid obtained from Corni fructus protected HEI-OC1 auditory cells from hydrogen peroxide cytotoxicity in a dose-dependent fashion. In addition, to investigate the protection mechanism of ursolic acid on hydrogen peroxide cytotoxicity toward HEI-OC1, we measured the effects of ursolic acid on lipid peroxidation and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in hydrogen peroxide treated cells. Ursolic acid (0.05 - 2 ${\mu}g/ml$) had protective effect against the hydrogen peroxide-induced HEI-OC1 cell damage and reduced lipid peroxidation in a dose-dependent manner. Pre-treatment with ursolic acid significantly attenuated the decrease in activities of CAT and GPX, but SOD activity was not affected by the ursolic acid or hydrogen peroxide. These results indicate that ursolic acid protects hydrogen peroxide-induced HEI-OC1 cell damage through inhibition of lipid peroxidation and induce the antioxidant enzymes CAT and GPX.

Keywords

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