Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antitumor Sterol Isolated from the Fruiting Body of Pleurotus eryngii

Pleurotus eryngii 로부터 항암물질의 분리

  • Lee Young-Hoon (Department of Applied Life Science, Gyeongsang National University) ;
  • Park Ki-Hun (Department of Microbiological Engineering, Chinju National University) ;
  • Lee Byong-Won (Department of Microbiological Engineering, Chinju National University) ;
  • Cho Yong-Un (Department of Applied Life Science, Gyeongsang National University) ;
  • Choi Young-Ju (Department of Food and Nutrition, Silla University) ;
  • Gal Sang-Wan (Department of Applied Life Science, Gyeongsang National University)
  • 이영훈 (진주산업대학교 미생물공학과) ;
  • 박기훈 (경상대학교 응용생명과학부) ;
  • 이병원 (경상대학교 응용생명과학부) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 식품영양학부) ;
  • 갈상완 (진주산업대학교 미생물공학과)
  • Published : 2006.04.01
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Abstract

Activity-guided fractionations led to the isolation of antitumor compound, ergosterol peroxide ($5{\alpha},\;8{\alpha}-epideoxy-24(R)-methylcholesta-6,\;22-dien-3{\beta}-ol$) from the fruiting body of Pleuratus eryngii that was cultivated artificially. This sterol structure was established by using spectroscopic methods ($^1H\;and\;^{13}C$ nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formular of $C_{28}H_{44}O_3$ displaying characteristic features of epidioxy sterols. The 50% inhibitory concentrations ($IC_{50}$) of ergosterol peroxide against human lung cancer cell line (A549) and human ovarian cell line (SK-OV3) were $7{\mu}M\;and\;14{\mu}M$, respectively. In the DNA fragmentation assay, the compound showed the programmed cell death causing the chromosomal DNA fragmentation. It reveals that ergosterol peroxide arrests G1 phase of the cell division cycle.

새송이버섯으로부터 활성추적법으로 항암활성이 있는 물질인 에르고스테롤 프록사이드를 분리하였다. 이 스테롤의 구조는 분광법과 NMR법으로 확인하였으며 분자식은 $C_{28}H_{44}O_3$이었다. 폐암과 난소암에 $IC_{50}$값은 각각 $7{\mu}M$과 $14{\mu}M$이었다. DNA단편화 실험에서 이 화합물은 암세포의 chromosimal DNA 를 사닥다리모양으로 분해하였고, 세포 분열주기의 억제실험에서 G1단계를 억제함을 관찰하였다.

Keywords

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