Journal of Soil and Groundwater Environment (한국지하수토양환경학회지:지하수토양환경)

Korean Society of Soil and Groundwater Environment (한국지하수토양환경학회)
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Detection of Biodegradative Genes in Oil Contaminated Soil Microbial Community by Oligonucleotide Microarray

Oligonucleotide Microarray를 이용한 유류 오염 토양 미생물 군집내 난분해성 화합물 분해 유전자의 검출

  • Lee Jong-Kwang (R & D Center, Samsung Engineering Co., Ltd.) ;
  • Kim Hee (R & D Center, Samsung Engineering Co., Ltd.) ;
  • Lee Doo-Myoung (R & D Center, Samsung Engineering Co., Ltd.) ;
  • Lee Seok-Jae (R & D Center, Samsung Engineering Co., Ltd.) ;
  • Kim Moo-Hoon (R & D Center, Samsung Engineering Co., Ltd.)
  • 이종광 (삼성엔지니어링(주) 기술연구소) ;
  • 김희 (삼성엔지니어링(주) 기술연구소) ;
  • 이두명 (삼성엔지니어링(주) 기술연구소) ;
  • 이석재 (삼성엔지니어링(주) 기술연구소) ;
  • 김무훈 (삼성엔지니어링(주) 기술연구소)
  • Published : 2006.02.01
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Abstract

The analysis of functional population and its dynamics on the environment is essential for understanding bioremediation in environment. Here, we report a method for oligonucleotide microarray for the monitoring of aliphatic and aromatic degradative genes. This microarray contained 15 unique and group-specific probes which were based on 100 known genes involved pathways in biodegradation. Hybridization specificity tests with pure cultures, strain Pseudomonas aeruginosa KCTC 1636 indicated that the designed probes on the arrays appeared to be specific to their corresponding target genes. It was found that the presence of 8 genes encoding alkane, naphthalene, biphenyl, pyrene (PAH ring-hydroxylating) degradation pathway could be detected in oil contaminated soil sample. Therefore, the findings of this study strongly suggest that oligonucleotide microarray is an effective diagnostic tool for evaluating biodegradation capability in oil contaminated subsurface environment.

환경 내에서 생물학적 복원을 이해하기 위해서는 미생물 기능성 군집 및 활성을 분석하는 것은 필수적이다. 본 연구에서는 유류오염 토양의 미생물 군집을 모니터링하기 위하여 난분해성 물질의 생물학적 분해에 관여하는 100개의 알려진 대사경로 및 유전자를 기반으로 한 oligonucleotide microarray를 개발하였다. 본 연구에 사용된 microarray는 유류오염 분해 대사에 관련된 유전자를 진단하기 위한 15개의 고유한 probe를 포함하고 있다. 디자인된 probe의 hybridization specificity는 표준 균주, Pseudomonas aeruginosa KCTC1636을 이용하여 확인 하였으며, 유류오염토양 시료의 분석결과 alkane, naphthalene, biphenyl, pyrene(PAH ring-hydroxylating) 분해에 관련된 8개의 유전자 발현을 확인 하였다. 이러한 결과는 DNA microarray가 유류오염토양환경에서 생물학적 분해유전자 진단에 효과적으로 이용될 수 있을 뿐만 아니라 생물학적 복원의 가능성을 진단하기에도 적합한 기법이라는 것을 나타내고 있다.

Keywords

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