KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Two-dimensional Electrophoretic Analysis of Nucleotide phosphate Kinase Mediated Hydrogen Peroxide Cross-linking in Saccharamyces cerevisiae

2-D 전기영동 분석을 통한 $H_2O_2$와 연계된 효모 시스템 NDPK에 관한 연구

  • Moon Hae-Jeong (Industrial Liaison Research Institute) ;
  • Yun Dae-Jin (Department of Chemical Engineering, Kyung Hee University) ;
  • Park Chang-Ho (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • 문혜정 (경희대학교 산학협력기술연구원) ;
  • 윤대진 (경상대학교 대학원 응용생명과학부) ;
  • 박창호 (경희대학교 환경.응용화학대학)
  • Published : 2006.02.01
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Abstract

Oxidative modification of nucleoside diphosphate kinase (NDPK) is identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The quaternary structure of NDPK appears to be regulated by cross-linking with an oxidant, $H_2O_2$. We compared roles of NDPK in each of wild type and ynk mutant against oxidative stress. Six specific proteins changed by $H_2O_2$ were identified using two-dimensional electrophoretic analysis. YNK regulated several proteins, related to $H_2O_2$ signaling functions. These results suggest that one of the important functions of NDPK is the regulation of cellular redox state.

최근의 연구에 의하면 열이나, 산화적 스트레스에 대해서 NDPK는 구조적인 변화를 유발하며, 효소 활성과 구조가 oxidant에 의해 변화된다는 보고를 근거로 하여 정상적인 효모균주와 효모의 NDPK 유전자가 파괴된 mutant에서, 산화적 스트레스에 관련된 역할을 규명하고자 2-D 전기영동 방법을 통해서, $H_2O_2$의 처리전과 처리 후에 전사패턴이 변화된 유전자들, 즉, 산화적조절 신호체제에 연관되어졌을 것이라고 생각되어지는 몇 개의 단백질 리스트를 얻었다. 이 결과는 NDPK의 redox state의 조절에 관련된 효소의 성질을 규명함에 있어 유용한 유전자 신호 체제정보를 제공할 것으로 생각되어진다.

Keywords

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