Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 38 Issue 3
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- Pages.152-157
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- 2006
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Usefulness of Automated PCR Test for Detection of Mycobacterium tuberculosis in Clinical Samples
임상검체별 결핵균 검출을 위한 자동화 중합효소연쇄반응 검사의 유용성
- Choi, Woo-Soon (Department of Laboratory Medicine, Daejeon St. Mary's Hospital, College of Medicine, the Catholic University) ;
- Shin, So-Young (Department of Laboratory Medicine, Daejeon St. Mary's Hospital, College of Medicine, the Catholic University)
- Published : 2006.12.31
Abstract
The purpose of this study was to evaluate the usefulness of the automated TB-PCR assay for the detection of Mycobacterium tuberculosis. The 807 cases were analyzed with their TB-PCR, AFB smear and culture in bronchial washing fluids, sputum and body fluids samples. The TB-PCR positive of the bronchial washing fluid, sputum and body fluids were 11.3%, 7.3% and 3.6%, respectively, in cases of AFB smear-negative and culture-negative. The sensitivity values of the bronchial washing fluid, sputum and body fluids were 93.3%, 100% and 50%, respectively, according to the culture result. The sensitivity of body fluids was lower than that of the bronchial washing fluid and sputum. The specificity values of the bronchial washing fluid, sputum and body fluids were 83.3%, 89.0% and 95.7%, respectively, according to the culture result. In conclusion, the automated TB-PCR assay proved to be a useful method for the detection of Mycobacterium tuberculosis in the bronchial washing fluid and sputum. But we think that there is still a need for us to study body fluids further.