Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Correlation between In vitro Plant Regeneration and Polarity with Boxthorn (Lycium chinense Mill.) Seedlings

구기자나무 유식물체를 이용한 식물체의 재분화와 극성과의 관계

  • Kwon Hye-Kyoung (Department of Biology, Kongju National University) ;
  • Cho Yi-Yun (Department of Biology Education, Kongju National University) ;
  • Yoon Eui-Soo (Department of Biology, Kongju National University)
  • 권혜경 (공주대학교 생명과학과) ;
  • 조이연 (공주대학교 생물교육학과) ;
  • 윤의수 (공주대학교 생명과학과)
  • Published : 2006.04.01
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Abstract

This experiment was conducted out to investigate the effect of plant growth regulators on callus and shoot formation. The callus formation was effective on 1/2 MS medium containing 2,4-D, while shoot formation was suppressed. Shoot formation and differentiation were the highest in combination 0.1 mg/L of IAA and 0.1 mg/L of BA. The polarity of explants was investigated from cotyledon, which excised 20% of each basal and terminal parts. Formation of shoot was induced from excised ends of the basal part. In excised ends of the basal part, callus was induced vigorously and shoots were produced lately. Root induction was easily achieved in 1/3 MS medium from the adventitious shoot and more than 90% of regenerated plantlets acclimatized successfully and flowered normally.

구기자나무로부터 캘러스의 형성과 shoot의 형성에 미치는 생장조절제의 영향을 연구한 결과 캘러스의 형성은 2,4-D를 포함하는 배지에서 효과적이었으나 shoot의 형성에는 억제효과를 보였다. Shoot의 형성은 0.1mg/L IAA + 0.1mg/L BA의 배지에서 형성률과 절편당 형성 개체수가 가장 높은 분화율을 보였다. 절편체의 부위에 극성에 다른 캘러스의 형성과 shoot의 재분화에 있어서는 자엽의 기부와 말단부를 20% 절제한 절편의 기부의 절단면에서는 shoot의 형성이, 하부의 절단면에서는 캘러스의 형성이 높았으며 시간이 경과함에 따라 하부에서도 shoot의 형성이 보였다. 재분화식물은 1/3 MS 배지에서 뿌리가 양호하게 발달하였으며 토양 순화과정에서도 90% 이상의 생존율을 보여 개화가 이루어졌다.

Keywords

References

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