Abstract
This study was conducted to investigate the efficient propagation method of Cyrtomium caryoptideum var. coreanum by sporophyte culture. The influence of origin of explant sources (rhizome, blade, or stipe) and homogenization of culture materials on shoot regeneration were investigated. As a result, only rhizome explant exhibited the organogenic capacity and the shoot regeneration was promoted by homogenization of culture material. Vigorous and excellent growth of multiple shoots was induced on the half-strength of inorganic salts containing MS medium. It was appeared that optimum nitrogen content of shoot regeneration was half-strength of nitrogen containing MS medium (30mM) and optimum sucrose concentration was 1%. Addition of $NaH_2PO_4$ to culture medium generally enhanced shoot multiplication and promoted growth of the regenerants. The organogenic capacity of homogenized rhizomes was especially promoted on medium supplemented with $5{\mu}M$ kinetin plus $5{\mu}M$ IBA. The incorporation of $0.1\sim0.2%$ activated charcoal on medium supplemented with growth regulators prevented the formation of multiple bud primordia - nodule-like bud clusters and improved the normal morphogenesis of sporophytes.
본 연구는 포자체 배양을 통하여 참쇠고비의 효율적인 번식방법을 구하고자 수행되었다. 배양절편의 기원(근경, 엽병, 엽신)과 절편의 균질화가 신초 계생에 미치는 영향을 조사한 결과, 오직 근경 조직에서만 기관발생능력이 관찰되었고, 식물체 재생은 배양재료를 균질화함으로써 더욱 촉진되었다. MS배지의 무기물 농도를 2배로 첨가한 배지에서 신초의 증식과 생장이 활발히 이루어졌다. 신초 재생에 적합한 배지의 질소함량은 MS배지의 1/2배 수준 이었고, 적정 sucrose농도는 1%였다. 또한 배지에 $NaH_2PO_4$를 첨가함으로써 재생된 식물체의 생육이 전반적으로 촉진되었다. 근경절편의 기관형성능력은 kinetin과 IBA를 혼용 처리함으로써 촉진되었으며, $0.1\sim0.2%$의 활성탄과 생장조절물질을 혼용 처리한 결과, 다수의 싹이 뭉친 듯이 보이는 primordia 형태의 발달이 억제되었고, 포자체의 정상적인 형태형성이 향상되었다.
