약학회지 (YAKHAK HOEJI)

  • 제50권2호
  • /
  • Pages.105-110
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  • 2006
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Tributyltin 화합물이 PC12 세포의 Dopamine 생합성 저해작용에 미치는 영향

Inhibitory Effects of Tributyltin Acetate on Dopamine Biosynthesis in PC12 Cells

  • Kim Yu-Mi (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Jae-Joon (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Myung-Koo (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University)
  • 발행 : 2006.04.01
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초록

The effects of tributyltin acetate (TBTA), one of the endocrine-disrupting organotin compounds, on dopamine biosynthesis in PC12 cells were investigated. Treatment of PC12 cells with TBTA at $0.05\sim0.25{\mu}M$ significantly decreased the intracellular dopamine content in a concentration-dependent manner ($IC_{50}$ value, $0.17{\mu}M$). Under these conditions, tyrosine hydroxylase (TH) activity and TH mRNA level were also decreased by $0.1{\mu}M$ TBTA at 24 h, and recovered there-after. In addition, treatment with L-DOPA at 20 and $50 {\mu}M$ increased the intracellular dopamine content in PC12 cells and the increase in dopamine content by L-DOPA was significantly abolished by TBTA at $0.1\sim0.2{\mu}M$. These results indicate that TBTA at $0.1\sim0.2{\mu}M$ causes the decrease in the basal dopamine content and abolishes the increase in dopamine content in L-DOPA-treated cells in part by the inhibition of TH gene expression and activity.

키워드

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