YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Lactic Acid Bacteria Isolated from Healthy Korean Having Antimicrobial Activity Against VISA and VRE

한국 성인에서 분리한 유산균의 VISA(Vancomycin-Intermediate Resistant Staphylococcus aureus)와 VRE(Vancomycin Resistant Enterococcus faecium)에 대한 성장 억제

  • Yun Ji-Hee (Department of Pharmacy, Sahmyook University) ;
  • Kim Yun-A (Department of Pharmacy, Sahmyook University) ;
  • Song Moon-Seok (Department of Pharmacy, Sahmyook University) ;
  • Kang Byung-Yong (Research Institute for Life Science, Sahmyook University) ;
  • Ha Nam-Joo (Department of Pharmacy, Sahmyook University)
  • Published : 2006.04.01
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Abstract

VISA and VRE are the main causes of surgical infection, urinary tract infections and bacteremia in hospitals. In this study; we selected VISA (Vancomycin Intermediate resistant Staphylococcus aureus) and VRE (Vancomycin Resistant Enterococcus) isolated from the clinical isolates. One of the isolated strains indicated the high resistance to severel anti-biotics (Vancomycin, Teicoplanin, Mupirocin, Synercid, Ciprofloxacin, Gentamicin, Lincomycin, Cefotaxim, Meropenem). Antimicrobial activity of Bifidobacterium spp. against VISA and VRE were measured. About $10^4$ cells of VISA or VRE were mixed with 1,5 and 9 ml of Bifidobacterium and the final volume was adjusted to 10 ml with brain heart infusion (BHI) broth. The cell suspension was incubated for 3, 6, 9, and 24 hr, serially diluted and then plated on BHI agar plate. As numbers of Bifidobacterium were increased viable cell count of VISA and VRE decreased. The strongest antimicrobial activity of the Bifidobacterium was observed after 9hr incubation in any mixture, almost completely inhibiting the growth of VISA and VRE.

Keywords

References

  1. Kwon, N. H., Kim, S. H., Bae, W. K., Kim, J. Y., Lim, J. Y., Noh, K. M., Kim, J. M., Ahn, J. S. and Park, Y. H. : Antimicrobial activity of Lactobacillus reuteri against major food-borne pathogens. J. Fd Hyg. Safety 16, 264 (2001)
  2. Molder, H. W., Mckeller, R. C. and Yaguchi, M. : Bifidogenic factors-sources, meta-bolism and applications. Int. Dairy J. 4, 383 (1994) https://doi.org/10.1016/0958-6946(94)90055-8
  3. Kim, J. B., Kim, G. H., Song, H. W., Park, S. U., Eom, Y. B., Park, S. W., Kim, Y. S. and Park, S. J. : Rapid detection of vancomycin resistant Enterococci using multiplex polymerase chain reactions. Korean J. Biome. Lab. Sci. 5, 95 (1999)
  4. Olsen, S. J., Bishop, R., Brenner, F. W., Roels, T. H., Bean, N., Tauxe, R. V. and Slutsker, L. : The chainging epidemiology of Salmonella; trends in serotypes isolated from humans in the United State 1987-1997. J. Infect. Dis. 183, 753 (2002) https://doi.org/10.1086/318832
  5. Uttley, A. H., Collins, C. H., Naidoo, J. and George, J. C. : Vancomycin-resistant enterococci. Lancet 1, 57 (1988) https://doi.org/10.1016/S0140-6736(01)18851-3
  6. Hsueh, P. R., Chen, W. H., Teng, L. J. and Luh, K. T. : Nosocomial infections due to methcillin-resistant Staphylococcus aureus and vamcomycin-resistant enterococci at a university hospital in Taiwan from 1991 to 2003 : resistance trends, antibiotic usage and in vitro activities of newer antimicrobial agents. Int. J. Antimicrob. Agents 26, 43 (2005) https://doi.org/10.1016/j.ijantimicag.2005.04.007
  7. Humphreys, H., Dolan, V., Sexton, T., Conlon, P., Rajan, L., Creamer, E., Walshe, J., Donohoe, J. and Symyth, E. G. : Implications of colonization of vancomycin-resistant enterococci (VRE) in renal dialysis patients. Learning to live with it- J. Hospital Infect. 58, 28 (2004) https://doi.org/10.1016/j.jhin.2004.04.022
  8. Nelason, R. R. S., McGregor, K. F., Brown, A. R., Amyes, S. G. B. and Young, H. K. : Isolation and characterization of glycopeptide-resistant Enterococci from hospitalised patients over a 30-month period. J. Chin. Microbiol. 38, 2112 (2000)
  9. Yoshida, O., Yasukata, T., Sumino, Y., Munekage, T., Narukawa, Y. and Nishitani, Y. : Novel semi-synthetic glycopeptide antibiotics active against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE): doubly-modified water-soluble derivatives of chloroorienticin B. Bioorganic & Medicinal Chemistry Letters 12, 3037 (2002) https://doi.org/10.1016/S0960-894X(02)00662-5
  10. LeClercq, R., Perlot, E., Duval, J. and Courvalin, P. : Plasmidmediated resistance to vancomycin and teicoplanin in Enterococcus faecium. N. Engl. J. Med. 319, 157 (1988) https://doi.org/10.1056/NEJM198807213190307
  11. Still, J., Law, E., Friedman, B., Fuhrman, S. and Newton, T. : Vancomycin-resistant organisms on a burn unit. South Med. J. 94, 810 (2001) https://doi.org/10.1097/00007611-200194080-00012
  12. Wiston, L. G., Charlebois, E. D., Pang, S., Bangsberg, D. R., Perdreau-Remington, F. and Chambers III, H. F. : Impact of a formulary switch from ticarcillin-clavulanate to piperacillintazobacttam on colonization with vancomycin-resistant enterococci. American J. Infect. Control 32(8), 462 (2004) https://doi.org/10.1016/j.ajic.2004.07.004
  13. Denis, O., Nonhoff, C., Byl, B., Knoop, C., Bobin-Dubreux, S. and Struelens, M. J. : Emergence of vancomycin-intermediate Staphylococcus aureus in a Belgian hospital : microbiological and clinical features. J. Antimicrob. Chemotherapy 50, 383 (2002) https://doi.org/10.1093/jac/dkf142
  14. Dobbin, G., Hariharan, H., Daoust, P. Y., Hariharan, S., Heaney, S., Coles, M., Price, L. and Muckle, C. A. : Bacterial flora of free-living Double-crested cormorant (Phalacrocorax auritus) chicks on Prince Edward Island, Canada, with reference to enteric bacteria and antibiotic resistance. Comp. Immnu. Microbiol. & Infect. Dis. 28, 71 (2005) https://doi.org/10.1016/S0147-9571(04)00059-1
  15. Fridkin, S. K. : Vancomycin-intermediate and -resistant Staphylococcus aureus : What the infectious disease specialist needs to know. Clin. Infect. Dis. 32, 108 (2001) https://doi.org/10.1086/317542
  16. Song, J. H. : Emerging infectious disease due to micorbial adaptation: Emergence and spread of antimicrobial resistance. Korean Society of Infection Disease, Infection and Chemotherapy 31, 79 (2002)
  17. Kheadr, E., Bernoussi, N., Lacroix, C. and Fliss, I. : Compariasion of the sensitivity of commercial strains and infant isolates of bifidobacteria to antibiotics and bacteriocins. Int. Dairay J. 14, 1041 (2004) https://doi.org/10.1016/j.idairyj.2004.04.010
  18. Kim, S. H., Keon, N. H., Kim, J. Y., Lim, J. Y., Bae, W. K., Kim, J. M., Noh, H. J., Jung, W. K., Park, K. T., Lee, J. E., Ra, J. C. and Park, Y. H. : Antimicrobial activity of natural product made by opuntia ficus-indica var. saboten against Salmonella spp. and Escherichia coli O157:H7. J. Fd Hyg. 17, 71 (2002)
  19. Bae, H. G., Kim, S. O., Parll, J. S., Kang, B. Y., Choi, S. S., Kang, C. Y. and Ha, N. J. : Analysis of Genomic diversity of Bifidobacterium spp. isolation from Korean adult. Yakhak Hoeji 48, 20 (2004)
  20. Chun, U. H., Park, B. S. and Cho, J. S. : Optimum conditions for the protoplast formation of Lactobacillus plantarum and Lactobacillus plantarum. Korean J. Botechnol. Bioeng. 9, 191 (1994)
  21. Lim, J. J. and Yun, H. I. : Postantibiotic effects and postantibiotic sub-MIC effects of erythromycin, roxithromycin, tilmicosin, and tylosin on Pasteurella multocida. Int. J. Antimicrob. Agents 17, 471 (2001) https://doi.org/10.1016/S0924-8579(01)00328-4
  22. Yun, J. H., Yim, D. S., Kang, J. Y., Kang, B. Y., Shin, E. A., Chung, M. J., Kim, S. D., Baek, D. H., Choi, S. S. and Ha, N. J. : Identification of Lactobacillus ruminus SPM 0211 isolated from healthy Korean and screening for the antimicrobial activity of this strain against some pathogens. Arch. Pharm. Res. 28, 660 (2005) https://doi.org/10.1007/BF02969355
  23. Lee, K. M., Yun, J. H., Choi, S. S. and Ha, N. J. : Comparison of environmental growth condition in Bifidobacterium spp. parent and It's mutants. J. of Life Sci. 12, 113 (2004)