Antidiabetic and Hypolipidemic Effects of Collybia confluens Mycelia Produced by Submerged Culture in Streptozotocin-Diabetic Rats

  • 발행 : 2006.01.01

초록

This investigation was undertaken to study the effects of oral administration (3 weeks) of Collybia confluens mycelial powder (CCMP) produced by a submerged culture on plasma glucose and other biochemical parameters in streptozotocin (STZ)-induced diabetic rats. Antidiabetic and hypolipidemic effects were proportionally increased with the increasing concentration of the CCMP for oral administration. The CCMP, at the dose of 400 mg/kg BW, substantially reduced the plasma glucose level by as much as 33.1 % as compared to the STZ-induced diabetic rats group. It also lowered the plasma total cholesterol, triglyceride, and low density lipoprotein (LDL) cholesterol by 22.9%, 19.9%, and 37.3%, respectively. The levels of total cholesterol and triglyceride in liver were reduced to the extent of by 13.5% and 18.8%, and the activity of alanine transaminase (ALT) and aspartate transaminase (AST) was decreased by 48.8% and 37.2%, respectively, under the influence of CCMP. The general components of CCMP were found to contain 26.18% carbohydrate, 3.67% crude ash, 4.02% crude fat, 22.55% crude protein, and 43.58% dietary fiber. The amino acid composition of the CCMP was also analyzed in detail.

키워드

참고문헌

  1. AOAC. Official methods of analysis (16th ed). Arlington, VA: Association of Official Chemists, (1995)
  2. Bar-on, H. and Roheim, P. S., Hyperlipidemia in streptozotocintreated rats. Diabetes, 25, 509-515 (1976) https://doi.org/10.2337/diabetes.25.6.509
  3. Betteridge, J., Lipid disorders in diabetes mellitus: In Pickup, J. C., Williams, G. (Eds.). Text Book of Diabetes. Second ed. Blackwell Science, London, pp. 55.1-55.31 (1997)
  4. Bursch, W. and Schulte, H. R., Cytoprotective effect of the prostacyclin derivative Hoprost against liver cell death induced by the hepatotoxins $CCl_4$ and bromobenzen. Klin. Wochenschr., 64, 47-50 (1986)
  5. Cho, Y. J., Kim, H. A., Bang, M. A., and Kim, E. H., Effects of dietary mushroom on blood glucose levels, lipid concentrations and glutathione enzymes in streptozotocin-induced diabetic rats. Kor. J. Nutr., 35, 183-191 (2002)
  6. Choi, J. S., Yokozawa, T., and Oura, V., Improvement of hyperglycemia and hyperlipidemia in streptozotocin-diabetic rats by a methanolic extract of Prunus davidiana stems and its main component, pruning. Planta Med., 57, 208-211 (1991) https://doi.org/10.1055/s-2006-960075
  7. Crisan, E. V. and Sands, A., Nutrition value. In Chang, S. T. & Hayes, W. A. (Eds). The biology and cultivation of edible mushrooms. Academic Press, New York, pp. 137-165 (1978)
  8. Duncan, D. M., Multiple-range tests for correlated and heteroscedastic means. Biometrics, 13, 164-170 (1957) https://doi.org/10.2307/2527799
  9. Frati, A. C., Gordillo, B. E., Altamirano, P., Ariza, C. R., Cortes- Franco, R., Chavez-Negrete, A., and Islas-Andrade, S., Influence of nopal intake upon fasting glycemia in type II diabetic and healthy subjects. Archivos de Investigation Medica., 22, 51-56 (1991)
  10. Folch, J., Lees, M., and Sloane-Stanley, G. H., A simple method for the isolation and purification of total lipid from animal tissue. J. Biol. Chem., 226, 497-509 (1957)
  11. Fridewald, W. T., Levy, R. I., and Fedreicson, D. S., Estimation of concentration of low density cholesterol in plasma, without use of preparative ultracentrifuse. Clin.Chem., 18, 499-508 (1979)
  12. Furuse, M., Kimura, C., Mabayo, R. T., Takahashi, H., and Okumara, J., Dietary sorbose prevents and improves hyperglycemia in genetically diabetic mice. J. Nutr., 123, 59-65 (1993) https://doi.org/10.1093/jn/123.1.59
  13. Gray, A. M. and Flatt, P. R., Insulin-releasing and insulin-like activity of Agaricus campestris (mushroom). J. Endocrinol., 157, 259-266 (1998) https://doi.org/10.1677/joe.0.1570259
  14. Johnson, I. T., The biological effects of dietary fiber in small intestine. In Dietary Fiber: Chemical and Biological Aspects, Southgate, A. T., Waldron, K., Johnson, I. T., and Fenwick, G. R., ed. the Royal Society of Chemistry, London, pp. 151-163 (1991)
  15. Junod, A. A., Lambert, E., Orci, L., Pictet, R., Gonet, A. E., and Renold, A. E., Studies of the diabetogenic action of streptozotocin. Proc. Soc. Exp. Biol. Med., 126, 201-205 (1967)
  16. Kiho, T., Itahashi, S., Sakushima, M., Matsunaga, T., Usui, S., Ukai, S., Mori, H., Sakamoto, H., and Ishiguro, Y., Polysaccharide in fungi. XXXVIII. Anti-diabetic activity and structural feature of galactomannan elaborated by Pestalotiopsis species. Biol. Pharm. Bull., 20, 118-121 (1997) https://doi.org/10.1248/bpb.20.118
  17. Kim, D. H., Yang, B. K., Hur, N. J., Das, S., Yun, J. W., Choi, Y. S., and Song, C. H., Hypoglycemic effects of mycelia produced from a submerged culture of Phellinus linteus (Berk. et Curt.) Teng (Aphyllophoromycetideae) in streptozotocin- induced diabetic rats. International Journal of Medicinal Mushrooms, 3, 21-26 (2001)
  18. Kim, S. H., Kim, H. W., Choi, E. C., and Kim, B. K., Immunologcial studies on Collyban of Collybia confluens. J. Kor. Cancer Association, 25, 288-298 (1993a)
  19. Kim, S. H., Kim, J. S., Jin, M. R., Kim, H. W., Choi, E. C., and Kim, B. K., Studies on antitumor components of Collybia confluens. Kor. J. Pharmacogn., 24, 267-281 (1993b)
  20. Laakso, M., Epidemiology of diabetic dyslipidemia. Diabetic. Rev., 3, 408-422 (1995)
  21. Lee, M. Y., Kim, M. K., Shin, J. G., and Kim, S. D., Dietary effect of hemicellulose from soy fiber on blood glucose and cholesterol content in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr., 33, 1119-1125 (2004) https://doi.org/10.3746/jkfn.2004.33.7.1119
  22. Nelson, R. W., Ihle, S. L., Lewis, L. D., Salisbury, S. K., Miller, T., Bergdall, V., and Bottoms, G. D., Effects of dietary fiber supplementation on glycemic control in dogs with alloxaninduced diabetes mellitus. Am. J. Vet. Res., 52, 2060-2066 (1991)
  23. Rakieten, N., Rakieten, M. L., and Nadkarni, M. V., Studies on the diabetogenic action of streptozotocin. Cancer Chemother. Rep., 29, 91-98 (1963)
  24. Sale, F. O., Marchesini, S., Fishman, P. H., and Berra, B. A., A sensitive enzymatic assay for determination of cholesterol in lipid extracts. Anal. Biochem., 142, 347-350 (1984) https://doi.org/10.1016/0003-2697(84)90475-5
  25. Sharma, S. R., Dwivedi, S. K., and Swarup, D., Hypoglycemic and hypolipidemic effects of Cinnamomum tamala Nees leaves. Indian J. Exp. Biol., 34, 372-374 (1996)
  26. Simon, B., Anke, T., Anders, U., Neuhaus, M., and Hansske, F., Collybial, a new antibiotic sesquiterpenoid from Collybia confluens (Basidiomycetes). Z. Naturforsch., (C) 50, 173-180 (1995)
  27. Van Horn, L. V. Lipid metabolism and choices for persons with diabetes: In 'Hand Book of Diabetes Medical Nutrition Therapy', Gaithersburg, Powers M, A., Aspen Publishers, Inc. Maryland, (1996)
  28. Waters Associates, Official method of amino acid analysis, Amino acid analysis system in the operation manual of Waters Associates, U.S.A., pp. 37 (1980)
  29. Yang, B. K., Kim, D. H., and Song, C. H., Production of Lentinus edodes mycelia in submerged culture and its hypoglycemic effect in diabetic rats. Kor. J. Mycology, 30, 131-135 (2002) https://doi.org/10.4489/KJM.2002.30.2.131
  30. Yang, B. K., Lee, H. J., Jeong, S. C., Lim, W. J., and Song, C. H., Hypoglycemic effect of Collybia confluens exobiopolymer produced by submerged mycelial culture on diabetic rats. J. Microbiol. Biotechnol., 15, 136-140 (2005) https://doi.org/10.1159/000087950
  31. Yang, B. K., Park, J. B., and Song, C. H., Hypolipidemic effect of exo-polymer produced in submerged mycelial culture of five different mushroom. J. Microbiol. Biotechnol., 12, 957-961 (2002)
  32. Yang, B. K., Wilson, M. A., Cho, K. Y., and Song, C. H., Hypoglycemic effect of an exo- and endo-biopolymer produced by submerged mycelial culture of Ganoderma lucidum in streptozotocin-induced diabetic rats. J. Microbiol. Biotechnol., 14, 972-977 (2004)
  33. Yuan, Z. M., He, P. M., Cui, J. H., and Takeuchi, H., Hypoglycemic effect of water-soluble polysaccharide from Auricularia auricula-judae Quel. on genetic diabetic KK-Ay mice. Biosci. Bitechnol. Biochem., 62, 1898-1903 (1998) https://doi.org/10.1271/bbb.62.1898