Toxicological Research

  • 제22권1호
  • /
  • Pages.31-37
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  • 2006
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

Melittin Inhibits Human Prostate Cancer Cell Growth through Induction of Apoptotic Cell Death

  • Park Hye-Ji (College of Pharmacy, Chungbuk National University) ;
  • Lee Yong-Kyung (College of Pharmacy, Chungbuk National University) ;
  • Song Ho-Seub (College of Oriental Medicin, Kyungwon University) ;
  • Kim Goon-Joung (College of Oriental Medicin, Kyungwon University) ;
  • Son Dong-Ju (College of Pharmacy, Chungbuk National University) ;
  • Lee Jae-Woong (College of Pharmacy, Chungbuk National University) ;
  • Hong Jin-Tae (College of Pharmacy, Chungbuk National University)
  • 발행 : 2006.03.01
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초록

It was previously found that melittin inhibited $NF-{\kappa}B$ activity by reacting with signal molecules of $NF-{\kappa}B$ which is critical contributor in cancer cell growth by induction of apoptotic cell death. We here investigated whether melittin inhibits cell growth of human prostate cancer cells through induction of apoptotic cell death, and the possible signal pathways. Melittin ($0{\sim}1\;{\mu}g/ml$) inhibited prostate cancer cell growth in a dose dependent manner. Conversely related to the growth inhibitory effect, melittin increased the induction of apoptotic cell death in a dose dependent manner. Melittin also inhibited DNA binding activity of $NF-{\kappa}B$, an anti-apoptotic transcriptional factor. Consistent with the induction of apoptotic cell death and inhibition of $NF-{\kappa}B$, melittin increased the expression of pro-apoptotic proteins caspase-3, and Bax but down-regulated anti-apoptotic protein Bcl-2. These findings suggest that melittin could inhibit prostate cancer cell growth, and this effect may be related with the induction of apoptotic cell death via inactivation of $NF-{\kappa}B$.

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