Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of irradiated frozen allogenic bone on bone formation in human fetal osteoblasts

사람태아골모세포에 대한 방사선조사 냉동 동종골의 골형성 유도효과

  • Hong, Ji-Yeon (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Jeong, Seong-Won (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Eom, Yu-Jeong (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Chae, Gyeong-Jun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Jeong, Ui-Won (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Chang-Seong (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Choe, Seong-Ho (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Jong-Gwan (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 홍지연 (원광대학교 치과대학 치주학교실) ;
  • 정성원 (원광대학교 치과대학 치주학교실) ;
  • 엄유정 (원광대학교 치과대학 치주학교실) ;
  • 채경준 (원광대학교 치과대학 치주학교실) ;
  • 정의원 (원광대학교 치과대학 치주학교실) ;
  • 김창성 (원광대학교 치과대학 치주학교실) ;
  • 최성호 (원광대학교 치과대학 치주학교실) ;
  • 김종관 (원광대학교 치과대학 치주학교실)
  • Published : 2006.09.30
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Abstract

The purpose of this study was to investigate the effects of irradiated frozen allogenic bone(IFAB) on the cell proliferation and differentiation of human fetal osteoblasts. Human fetal osteoblasts(hFOB1) were cultured to examine the cellular proliferation for 3 days and 5 days with $1mg/m{\ell}$, $100{\mu}g/m{\ell}$, $10{\mu}g/m{\ell}$, $1{\mu}g/m{\ell}$, $100ng/m{\ell}$, $10ng/m{\ell}$, $1ng/m{\ell}$ of IFAB, and to compare the ALP synthesis to control groups for 3 days with DMEM/F-12 1:1 Mixture and $1mg/m{\ell}$, $100{\mu}g/m{\ell}$, $10{\mu}g/m{\ell}$, $1{\mu}g/m{\ell}$, $100ng/m{\ell}$, $10ng/m{\ell}$, $1ng/m{\ell}$ of IFAB. To compare the calcium accumulation, hFOBl cultured for 23 days were quantified and photographed. The cellular proliferation of hFOBls treated with IFAB was increased at 5 days to control(p<0.05). The activity of ALP in hFOBls treated with $100ng/m{\ell}$ IFAB was significantly increased at 5 days(p<0.05). A quantified calcium accumulation in hFOBl was significantly increased at $100ng/m{\ell}$, $10ng/m{\ell}$ of IFAB(p<0.05). In the present study, we found that IFAB playa important role of bone formation in the early stage. There was considered that IFAB could be used in the bone graft material.

Keywords

References

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