Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Regulation of cementoblast differentiation and mineralization using conditioned media of odontoblast

상아모세포의 조건배지를 이용한 백악모세포의 분화와 석회화 조절

  • Moon, Sang-Won (Dept, of Oral Histology, College of Dentistry, Chosun University) ;
  • Kim, Hye-Sun (Dept, of Oral Histology, College of Dentistry, Chosun University) ;
  • Song, Hyun-Jung (Dept, of Oral Histology, College of Dentistry, Chosun University) ;
  • Choi, Hong-Kyu (Dept, of Oral Histology, College of Dentistry, Chosun University) ;
  • Park, Jong-Tae (Dept, of Oral Histology, College of Dentistry, Chosun University) ;
  • Kim, Heung-Joong (Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Jang, Hyun-Seon (Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Park, Joo-Cheol (Dept, of Oral Histology, College of Dentistry, Oral Biology Research Institute, Chosun University)
  • 문상원 (조선대학교 치과대학 구강조직학 교실) ;
  • 김혜선 (조선대학교 치과대학 구강조직학 교실) ;
  • 송혜정 (조선대학교 치과대학 구강조직학 교실) ;
  • 최홍규 (조선대학교 치과대학 구강조직학 교실) ;
  • 박종태 (조선대학교 치과대학 구강조직학 교실) ;
  • 김흥중 (조선대학교 치과대학 구강생물학연구소) ;
  • 장현선 (조선대학교 치과대학 구강생물학연구소) ;
  • 박주철 (조선대학교 치과대학 구강조직학 교실, 구강생물학연구소)
  • Published : 2006.06.30
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Abstract

For the regeneration of periodontal tissues, the microenvironment for new attachment of connective tissue fibers should be provided, At this point of view, cementum formation in root surface plays a key role for this new attachment. This study was performed to figure out which factor promotes differentiation of cementoblast Considering anatomical structure of tooth, we selected the cells which may affect the differentiation of cementoblast - Ameloblast, OD11&MDPC23 for odontoblasts, NIH3T3 for fibroblsts and MG63 for osteoblasts. And OCCM30 was selected for cementoblast cell line. Then, the cell lines were cultured respectively and transferred the conditioned media to OCCM30. To evaluate the result, Alizarin red S stain was proceeded for evaluation of mineralization. The subjected mRNA genes are bone sialoprotein(BSP), alkaline phosphate(ALP) , osteocalcin(OC), type I collagen(Col I), osteonectin(SPARC ; secreted protein acidic and rich in cysteine). Expression of the gene were analysed by RT-PCR, The results were as follows: 1. For alizarin red S staining, control OCCM30 didn't show any mineralized red nodules until 14 days. But red nodules started to appear from about 4 days in MDPC-OCCM30 & OD11-OCCM30. 2. For results of RT-PCR, ESP mRNAs of control-OCCM30 and others were expressed from 14 days, but in MDPC23-OCCM30 & OD11-OCCM30 from 4 days. Like this, the gene expression of MDPC23-OCCM30 & OD11-OCCM30 were detected much earlier than others. 3. For confirmation of odontoblast effect on cementoblast, conditioned media of osteoblasts(MG63) which is mineralized by producing matrix vesicles didn't affect on the mineralized nodule formation of cementoblasts(OCCM30). This suggest the possibility that cementoblast mineralization is regulated by specific factor in dentin matrix protein rather than matrix vesicles. Therefore, we proved that the dentin/odontoblast promotes differentiation/mineralization of cementoblasts. This new approach might hole promise as diverse possibilities for the regeneration of tissues after periodontal disease.

Keywords

References

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