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Response to Fungicides of Colletotrichum Species Isolated from Infected Tissues of Several Crops

다양한 작물로부터 분리한 탄저병균(Colletotrichum spp.)의 살균제에 대한 반응

  • Kim Joon-Tae (Department of Plant Medicine, College ol Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Min Ji-Young (Department of Plant Medicine, College ol Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim Heung-Tae (Department of Plant Medicine, College ol Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 김준태 (충북대학교 농업생명환경대학 식물의학과) ;
  • 민지영 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2006.04.01

Abstract

This study has been conducted to investigate the responses of various isolates of four Colletotrichum species such as C. gloeosporioides, C acutatum, C. coccodes, and C. dematium isolated from infected tissues of several crops to fungicides such as carbendazim, carbendazim+diethofencarb, four protective fungicides, and three ergosterol biosynthesis-inhibiting (EBI) fungicides. All the isolates of C. acutatum showed $EC_{50}$ values in a range of 0.001-3.040 ${\mu}g/ml$ against carbandazim, a benzimidazole fungicide. As for the response to carbendazim, the isolates of C. gloeosporioides obtained from pepper, apple, and strawberry were clearly divided into two groups, resistant or sensitive isolates. All the resistant isolates showed $EC_{50}$ values above 1000 ${\mu}g/ml$, whereas the sensitive isolates had lower $EC_{50}$ values than 0.550 ${\mu}g/ml$. The isolates of C. gloeosporioides exhibited a negative cross resistance between carbendazim and diethofencarb (a N-phenylcarbamate fungicide), but isolates of C. acutatum did not. Toward carbendazim, C. coccodes and C. dematium isolates showed a similar response to C. acutatum isolates and the sensitive isolates of C. gioeosporioides, respectively. As for response of Protective fungicides, all the isolates of C. acutatum showed a more resistant reaction than all the isolates of C. gloeosporioides. However, there was no difference among 4 species of Colletotrichum against EBI fungicides.

다양한 작물로부터 분리한 4종의 탄저병균, C. gloeosporioided, C. acutatum, C. coccodes 및 C. dematium 등의 균주들에 대하여 여러 가지 살균제, 즉 carbendazim, carbendazim과 diethofencarb와의 혼합제, 4개의 보호용 살균제 및 3개의 ergosterol 생합성 저해(EBI) 살균제 등에 대한 약제 반응을 조사하였다. Benzimidazole계에 속하는 carbendazim에 대해서 4종의 Colletotrichum의 반응은, C. acutatum은 사용한 6개의 균주 모두 $EC_{50}$값은 $0.001{\sim}3.04{\mu}g/ml$ 사이에 있었으며, 모든 균주의 MIC 값은 $100{\mu}g/ml$ 이상이었다 고추와 사과, 딸기에서 분리한 C. gloeosporioides는 carbendazim에 대해서 저항성과 감수성 반응이 뚜렷하게 구별되었는데, 고추의 두 균주는 $EC_{50}$값은 0.24와 $0.01{\mu}g/ml$이었으며 MIC 값 역시 $1.0{\mu}g/ml$ 이었다. 이러한 반응은 사과와 딸기에서 분리한 균주 중에서 감수성 반응을 보이는 균주인 B13과 138, 그리고 CGF89와 94, 252에서도 비슷한 경향을 보였다. 그러나 저항성 균주로 판명된 B90, 92, 147과 CGF50, 54, 222는 $EC_{50}$값과 MIC 값 모두가 $100{\mu}g/ml$를 상회하였다. C. gloeosporioides는 carbendazim과 역상관 교차 저항성을 보이는 diethofencarb와 carbendazim의 혼합제에 대해서는 모두 $1{\sim}100{\mu}g/ml$의 MIC값을 보였지만, C. acutatum은 $100{\mu}g/ml$이상의 MIC값을 보였다. Carbandazim에 대하여 C. coccodes는 C. acutatum과 그리고 C. dematium는 C. gloeosporioides의 감수성 균주들의 반응과 유사한 반응을 보였다. 4종의 보호용 살균제에 대해서는 C. acutatum은 C. gloeosporioides보다도 높은 저항성인 반응을 보였다. 그러나 ergosterol 생합성 저해 살균제에 대해서는 4종의 탄저병균이 일정한 경향을 보였다.

Keywords

References

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