Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Effect of Zooplankton Exposures on the Biomass and Intracellular Microcystin in Microcystis aeruginosa and Planktothrix agadhii

동물플랑크톤 노출 강도가 유해남조 Microcystis aeruginosa와 Planktothrix agardhii의 생체량 및 세포내 microcystin함량변화에 미치는 영향

  • Jang, Min-Ho (National Institute for Environmental Studies) ;
  • Jung, Jong-Mun (Pusan Water Quality Institute, Waterworks HQ) ;
  • Joo, Gea-Jae (Department of Biology, Pusan National University)
  • 장민호 (일본국립환경연구소) ;
  • 정종문 (부산시 상수도사업본부 수질연구소) ;
  • 주기재 (부산대학교 생물학과)
  • Published : 2006.06.30
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Abstract

This study was to evaluate microcystin production by two strains of cyanobacteria (Microcystis aeruginosa and Planktothrix agardhii) in response to three different levels of direct (0,4,8 inds.) or indirect (0,25, 50% of zooplankton culture media filtrate) exposures to zooplankton (Daphnia magna and Moina macrocopa). The cell biomass and intracellular microcystin (MC) were measured everyday. The survival rates of zooplankton were evaluated for daily intervals for the direct exposure. The intracellular MC produced peaked on the day 3 or 4, and then decreased over the both exposure experiment. In the direct experiment, the MC values were significantly different among the control and zooplankton treatments (ZT; repeated measures-ANOVA: P< 0.039). The MC contents of P. agardhii strain (No.204) were significantly higher (Tukey test, P< 0.082) in ZT2 (8 inds.) than in ZT2 (4 inds.). On the peak day, the intracellular MC exposed to both zooplanktons was significantly higher than the control (One-way ANOVA, P< 0.021). Higher zooplankton survivals were observed in the M. aeruginosa strain (No. 111) rather than in high toxic P. agardhii strain. In the indirect experiment, the intracellular MC of the M. aeruginosa strain was significantly different among the control and zooplankton culture media filtrate (ZCMF)treatments (rm-ANOVA: P<0.004), The MC exposed ZCMF2 (50%) were significantly higher than in ZCMFI (25%: Tukey test, P< 0.025) for both strains. This study strongly supports the induced-defensive MC production of potentially toxic cyanobacteria in response to the presence of zooplankton.

두 종의 초식성 동물플랑크톤(Daphnia magna. Moina macrocopa)의 직접노출 밀도차(0, 4, 8개체)와 동물플랑크톤 배양여과액(ZCMF) 농도차(0, 25, 50%)에 따른, 두남조 Microcystis aeruginosa와 Planktothrix agardhii의 생체량과 세포내 microcystin(MC)을 1일 간격으로 측정하였고, 직접노출실험의 경우 동물플랑크톤 생존륜을 1일 간격으로 관찰하였다. 동물플랑크톤 직접노출 실험 결과, 두 균주에서 세포내 MC함량이 증가하였으며 (rm-ANOVA: P< 0.039), P. agardhii균주에서는 4개체 처리군(ZT1)보다 8개체 처리군(ZT2)에서 통계적으로 유의한 수준으로 높은 세포내 MC값이 관찰되었다(Tukey test. P<0.082).세포내 MC가 최고값을 보인 3일 또는 4일째에, 처리군들은 대조군에 비하여 통계적으로 유의한 차이를 보였다(One-way ANOVA, P< 0.021). ZCMF에 노출시킨 M. aeruginosa균주에서 세포내 MC함량이 통계적으로 유의한 수준으로 차이가 있었다(rm-ANOVA: P< 0.004), 실험기간동안 세포내 MC값은 M. aeruginosa균주에서 25%처리군 보다 50%처리군에서 통계적으로 유의한 수준으로 높게 나타났다(Tukey test, Plt; 0.025). 본연구결과, 세포내 MC량은 초식성 동물플랑크톤의 직접노출 밀도차와 분비화학물질(infochemical)농도차에 의존하여 증가 혹은 변화될수 있는 가능성이 있는 것으로 나타났으며, 부영양호에서 독성 남조 번성 조절시, 이러한 가능성을 고려하여 생물조절이 이루어져야 할 것으로 보인다.

Keywords

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