대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)
- 제19권2호
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- Pages.199-213
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- 2006
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- 1229-4292(pISSN)
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- 2508-3619(eISSN)
삼릉(三稜)이 자궁근종세포의 증식억제와 세포자멸사 관련 발현에 미치는 영향
The effect of Sparganii Rhizoma on the proliferation inhibition of human uterine leiomyoma cell and expression of gene related cell apoptosis
- Park, Chang-Gun (Dep. of gynecology, College of Oriental Medicine, Daeguhaany University) ;
- Baek, Seung-Hee (Dep. of gynecology, College of Oriental Medicine, Daeguhaany University) ;
- Kim, Dong-Chul (Dep. of gynecology, College of Oriental Medicine, Daeguhaany University)
- 발행 : 2006.05.31
초록
Purpose : This study was aimed to investigate the inhibitory effect of Sparganii Rhizoma on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis. Methods : This study was evaluated the number of death cells treated with indicated concentration of Sparganii Rhizoma and investigated cell death rate by MTS assay. Furthermore, fluorescence-activated cell sorter analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis. and then we observed the differential gene expression by western blot analysis. Results :1) The inhibitory effect on the growth of uterine leiomyoma cell treated with Sparganii Rhizoma was increased in a dose dependent manner. 2) As the result of FACS analysis, subG1 phase incrase was observed 23.49% inuterine leiomyoma cell treated with Sparganii Rhizoma at