The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Hwaganjeon on Immobilization-Stress or Cold-Stress in Mice

화간전(化肝煎)이 생쥐의 Immobilization-Stress 및 Cold-Stress에 미치는 영향(影響)

  • Park, Jong-Moon (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Ko, Jeong-Min (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Ahn, Kyu-Hwan (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Choe, Chang-Min (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Yoo, Sim-Keun (Department of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University)
  • 박종문 (원광대학교 한의과대학 부인과학교실) ;
  • 고정민 (원광대학교 한의과대학 부인과학교실) ;
  • 안규환 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실) ;
  • 유심근 (원광대학교 한의과대학 부인과학교실)
  • Published : 2006.11.30
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Abstract

Purpose : This study is to examine the effects of Hwaganjeon water extract(HGJ) on immobilization-stress or cold-stress in BALB/C mice. Methods : We have Hwaganjeon water extract(HGJ) by freeze-dryer & melt it by a saline solution. We feed HGJ 500mg/Kg to 5mice, and add immobilization-stress by putting mice in plastic cylinder 10 hours, and add cold-stress by putting mice in $4^{\circ}C$ cold room 6 hours. Results : 1. HGJ decreased the serum level of histamine and corticosterone increased by immobilization-stress or cold-stress. HGJ inhibited the release of histamine from mast cells at the concentration of 1 mg/ml. 2. HGJ did not affect the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but increased the cell viability of splenocytes decreased by immobilization-stress or cold-stress. 3. HGJ decreased the population of splenic $CD4^{+}$ and $CD8^{+}$cells increased by immobolization-stress or cold-stress. 4. HGJ enhanced the production of ${\gamma}$-interferon(IFN) and interleukin(IL)-2 decreased by immobilization-stress or cold-stress. Conclusion : These results indicate that HGJ may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.

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