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The Pharmaceutical Society of Korea (대한약학회)
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Effects of (+)-Eudesmin from the Stem Bark of Magnolia kobus DC. var. borealis Sarg. on Neurite Outgrowth in PC12 Cells

  • Yang, Yoo-Jung (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Park, Jae-In (College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Lee, Hak-Ju (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Seo, Seon-Mi (College of Life and Environment Sciences, Korea University) ;
  • Lee, Oh-Kyu (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Choi, Don-Ha (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Paik, Ki-Hyon (College of Life and Environment Sciences, Korea University) ;
  • Lee, Myung-Koo (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2006.12.31
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Abstract

(+)-Eudesmin [4,8-bis(3,4-dimethoxyphenyl)-3,7 -dioxabicyclo[3.3.0]octane] was isolated from the stem bark of Magnolia kobus DC. var. borealis Sarg. and found to have neuritogenic activity. $50\;{\mu}M$ (+)-eudesmin induced neurite outgrowth and enhanced nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. At this concentration, (+)-eudesmin also enhanced NGF-induced neurite-bearing activity and this activity was partially blocked by various protein kinase inhibitors. These included PD98059, a mitogen-activated protein kinase (MAPK) kinase inhibitor. GF109203X, a protein kinase C (PKC) inhibitor and H89, a protein kinase A (PKA) inhibitor. These results suggest that (+)-eudesmin can induce neurite outgrowth from PC12 cells by stimulating up-stream MAPK, PKC and PKA pathways.

Keywords

References

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