Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of DNA Conformation in the Particles of Bacteriophage P4 Mutant, P4 ash8

박테리오파아지 P4 ash8 sid71 입자 내 DNA 형태 분석

  • Song, Jae-Ho (Department of Life Science, College of Natural Science, Sunmoon University) ;
  • Kim, Kyoung-Jin (Department of Life Science, College of Natural Science, Sunmoon University)
  • 송재호 (선문대학교 자연과학대학 생명과학과) ;
  • 김경진 (선문대학교 자연과학대학 생명과학과)
  • Published : 2006.03.01
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Abstract

To study the packaging mechanism of the bacteriophage P2-P4 system which is a useful experimental tool for the study of viral capsid assembly, we analyzed the DNA contents of P4 sid- mutant, P4 ash8 sid71's phage particles. Two kind of particles having different density were separated by the CsCl buoyant equilibrium density gradient experiment with fresh made stock of P4 ash8 sid71. The DNA from each particles was prepared and its conformations was analyzed by electrophoresis. Unexpectedly, both particles contain not only dimeric and trimeric but also monomeric P4 DNA.

바이러스 조립 과정 기작 연구를 위한 좋은 재료인 박테리오파아지 P2-P4 시스템의 packaging 기작 연구를 위해, 머리 크기 조절 유전자 변이체인 P4 ash8 sid7l의 파아지 입자가 함유하고 있는DNA의 형태를 분식하였다. 파아지 stock을 준비하고 이것의 CsCl부양균등 밀도 편차실험을 수행하여, 밀도가 다른 두개의 입자들로 분리하였다. 각 입자의 DNA를 분리한 후, 전기영동을 통해 함유된 DNA의 형태를 확인하였다. 예상과 달리, 각 입자들은 dimer, trimer 뿐 아니라 monomer도 함유하고 있다는 새로운 사실을 발견하였다.

Keywords

References

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