한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제39권1호
  • /
  • Pages.49-54
  • /
  • 2006
  • /
  • 0374-8111(pISSN)
  • /
  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
DOI QR코드

DOI QR Code

독성 Alexandrium tamarense를 섭취한 담치류 4종의 마비성패독 축적

Paralytic Shellfish Poisoning Toxin Accumulation in Four Mussel Species Fed on Toxic Alexandrium tamarense

  • 김영수 (부경대학교 수산생물학과) ;
  • 손명백 (부경대학교 수산생물학과) ;
  • 김창훈 (부경대학교 수산생물학과)
  • Kim, Young-Soo (Department of Fisheries Biology, Pukyong National University) ;
  • Shon, Myung-Back (Department of Fisheries Biology, Pukyong National University) ;
  • Kim, Chang-Hoon (Department of Fisheries Biology, Pukyong National University)
  • Published : 2006.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

Abstract

Cultured cells of the toxic Alexandrium tamarense were fed to four mussel species, Mytilus coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus, to examine the interspecies and interlocality differences in the ability to accumulate paralytic shellfish poisoning (PSP) toxins. Toxin content of A. tamarense cells varied during culture period. In contrast, toxin composition in the cell (C1,2, GTX1-4 and neoSTX) was constantly stable. In feeding experiment, the four mussel species collected from Geoje intoxicated after uptake of A. tamarense. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) of M. coruscus, M. edulis, M. galloprovincialis and Septifer vulgatus were $1,660{\pm}79,\;3,914{\pm}2,242,\;5,626{\pm}1,620\;and\;958{\pm}163$, respectively. Toxin profiles included C1,2, GTX1,4 and neoSTX as the major components, and dcGTX2,3, GTX2,3, neoSTX and STX as the minor ones. Toxin accumulation of three mussel species collected from Pohang, Geoje and Anmyon-do showed interspecies and interlocality differences. Toxin content ($average{\pm}SD\;{\mu}g$ STXeq/100 g) were $91{\pm}4,\;151{\pm}14,\;39{\pm}3$ in M coruscus, $189{\pm}1,\;231{\pm}11,\;206{\pm}15$ in M edu/is and $214{\pm}28,\;326{\pm}30,\;291{\pm}26$ in M. galloprovincialis in order of Anmyon-do, Geoje and Pohang.

Keywords

References

  1. AOAC. 2000. Paralytic shellfish poison. In: Official methods of analysis of the AOAC (17th). Willianm, H. ed. Association of Official Analytical Chemist, Virginia, 59-60
  2. Bricelj, V.M., J.H. Lee, A.D. Cembella and D.M. Anderson. 1990. Uptake kinetics of paralytic shellfish toxins from the dinoflagellate Alexandrium fundyense in the mussel Mytilus edulis. Mar. Ecol. Prog. Ser., 63, 177-188 https://doi.org/10.3354/meps063177
  3. Bricelj, V.M., J.H. Lee and A.D. Cembella. 1991. Influence of dinoflagellate cell toxicity on uptake and loss of paralytic shellfish toxins in the northern quahog Mercenaria mercenaria. Mar. Ecol. Prog. Ser., 74, 33-46 https://doi.org/10.3354/meps074033
  4. Bricelj, V.M. and S.E. Shumway. 1998. Paralytic shellfish toxins in bivalve mollusks: occurrence, transfer kinetics, and biotransformation. Rev. Fish. Sci., 6, 315-383 https://doi.org/10.1080/10641269891314294
  5. Cembella, A.D., J.J. Sullivan, G.L. Boyer, F.J.R. Taylor and R.J. Anderson. 1987. Variation in paralytic shellfish toxin composition within the Protogonyaulax tamarensis/catenella species complex: red-tide dinoflagellates. Biochem. Syst. Ecol., 15, 171-186 https://doi.org/10.1016/0305-1978(87)90018-4
  6. Cembella, A.D., S.E. Shumway and N.I. Lewis. 1993. Anatomocal distribution and spatio-temporal variation in paralytic shellfish toxin composition in two bivalve species from the Gulf of Maine. J. Shellfish Res., 12, 389-403
  7. Chang, D.S., I.S. Shin, J.H. Pyeon and Y.H. Park. 1987. A study of paralytic shellfish poison of sea mussel, Mytilus edulis - food poisoning accident in Gamchun Bay, Pusan, Korea, 1986. Bull. Kor. Fish. Soc., 20, 293-299
  8. Chebib, H.A., A.D. Cembella and P.Anderson. 1993. Differential paralytic shellfish toxin accumulation and detoxification kinetics in transplanted populations of Mytilus edulis exposed to natural blooms of Alexandrium excavatum. In: Toxic Phytoplankton Blooms in the Sea. Smayda, T.J. and Y. Shimizu, eds. Elsevier, New York, 383-388
  9. Frank, P.J.S. and D.M. Anderson. 1992. Toxic phytoplankton blooms in the southwestern Gulf of Maine: testing hypotheses of physical control using historical data. Mar. Biol., 112, 165-174 https://doi.org/10.1007/BF00349740
  10. Guillard, R.R.L. and J.H. Ryther. 1962. Studies of marine planktonic diatoms. I Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol., 8, 229-239 https://doi.org/10.1139/m62-029
  11. Hallegraeff, G.M. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia, 32, 79-99 https://doi.org/10.2216/i0031-8884-32-2-79.1
  12. Hallegraeff, G.M., C.J. Bolch, J. Bryan and B. Koerbin. 1990. Microalgal spores in ship's ballast water: A danger to aquaculture. In: Toxic Marine Phytoplankton. Graneli, E., B. Sundstorm, L. Edler, and D.M. Anderson, eds. Acedemic Press, New York, 475-480
  13. Han, M.S., J.K. Jeon and Y.H. Yoon. 1993. Distribution and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dinoflagellate) in the southeastern coastal waters, Korea. Algae, 8, 7-13
  14. Kim C.H. 1995. Paralytic shellfish toxin profiles of the dinoflagellate Alexandrium species isolated from benthic cysts in Jinhae Bay, Korea. J. Korean Fish. Soc., 28, 364-372
  15. Kim, C.H. and Shin, J.B. 1997. Harmful and toxic red tide algal development and toxins production in Korean coastal waters. Algae, 12, 269-276
  16. Lee, J.S., I.S. Shin, Y.M. Kim and D.S. Chang. 1997. Paralytic shellfish toxins in the mussel, Mytilus edulis, caused the shellfish poisoning accident at Geoje, Korea, in 1996. J. Kor. Fish. Soc., 30, 158-160
  17. Oshima, Y., W.E. Fallon, Y. Shimizu, T. Noguchi and Y. Hashimoto. 1976. Toxins of the Gonyaulax sp. and infested bivalves in Owase Bay. Bull. Jap. Soc. Sci. Fish., 42, 851-856 https://doi.org/10.2331/suisan.42.851
  18. Oshima, Y., K. Sugino, H. Itakura, M. Hirota and T. Yasumoto. 1990. Comparative studies on paralytic shellfish toxin profile of dinoflagellate and bivalves. In: Toxic Marine Phytoplankton. Graneli, E., B. Sundstorm, L. Edler, and D.M. Anderson, eds. Acedemic Press, New York, 391-396
  19. Oshima, Y. 1995a. Post-column derivazation HPLC methods for paralytic shellfish poison. In: Manual on Harmful Marine Microalgae. Hallegraeff, G.M., D.M. Anderson and A.D. Cembella, eds. UNESCO, Paris, 81-94
  20. Oshima. Y. 1995b. Chemical and enzymatic transformation of paralytic shellfish toxins in marine organisms. In: Harmful Marine Algal Blooms. Lassus, P., G. Arzul, E. Erard-Le-Denn, P. Gentien and C. Marcaillou- Le-Baut, eds. Lavoisier, Paris, 475-480
  21. Park, T.G., C.H. Kim and Y. Oshima. 2004a. Paralytic shellfish toxin profiles of different geographic populations of Gymnodinium catena tum (Dinophyceae) in Korean coastal waters. Phycol. Res., 52, 300-305 https://doi.org/10.1111/j.1440-1835.2004.tb00339.x
  22. Park, H.S., S.K. Yi and S.G. Paik. 2004b. Assessment of the productivity of Mytilus galloprovincialis on the mussel culture ground in Jinhae Bay. Kor. J. Malacol., 20, 45-53
  23. Prakashi, A., J.C. Medcof and A.D. Tennant. 1971. Paralytic shellfish poisoning in eastern Canada. Fish. Res. Bd. Can. Bull., 177, 1-87
  24. Sekiguchi, K., S. Sato, S. Kaga, T. Ogata and M. Kodama. 2001. Accumulation of paralytic shellfish poisoning toxins in bivalves and an ascidian fed on Alexadnrium tamarensecells. Fish. Sci., 67,301-305 https://doi.org/10.1046/j.1444-2906.2001.00228.x
  25. Shimizu, Y. and M. Yoshioka. 1981. Transformation of paralytic shellfish toxins as demonstrated in scallop homogenates. Science, 212, 547-546 https://doi.org/10.1126/science.7209548
  26. Shumway, S.E. and T.L. Cucci. 1987. The effects of the toxic dinoflagellate Protogonyaulax tamarense on the feeding and behavior of bivalve molluscs. Aquat. Toxicol., 10, 9-27 https://doi.org/10.1016/0166-445X(87)90024-5
  27. Shumway, S.E. 1990. A review of the effects of algal blooms on shellfish and aquaculture. World Aqua- cult. Soc., 21. 65-104 https://doi.org/10.1111/j.1749-7345.1990.tb00529.x
  28. Sullivan, J.J., W.T. Iwaoka and J. Liston. 1983. Enzymatic transformation of PSP toxins in the littleneck clam (Protochaca staminea). Biochem. Biophys. Res. Commun., 114, 465-472 https://doi.org/10.1016/0006-291X(83)90803-3
  29. Taylor, F.J.R., Y Fukuyo and J. Larsen. 1995. Taxonomy of harmful dinoflagellate. In: Manual on Harmful Marine Microalgae. Hallegraeff G.M., D.M. Anderson and A.D. Cembella, eds. UNESCO, Paris, 283-317
  30. Twarog, B.M. 1974. 'Immunity' to paralytic shellfish toxin in bivalve molluscs. In: Proceedings of - the 2nd International Coral Reef Symposium. 1. Great Barrier Reef Committee, Brisbane, 505-512
  31. White, A.W., S.E. Shumway, J. Nassif and D.K. Whittaker. 1993. Variation in level of paralytic shellfish toxins among individual shellfish. In: Toxic Phytoplankton Blooms in the Sea. T.J. Smayda and Y. Shimizu, eds. Elsevier, New York, 441-446
  32. Yoo, S.K., H.S. Lim and Y.J. Chang. 1990. On the occurrence of the larvae, spatfall and early growth of mussel Mytilus edulis, in Chinhae Bay. Kor. J. Malacol., 6, 1-10