Antifungal Activity of Bacillus sp. Against Phytophthora infestans

  • Kim Hye-Sook (Bio/Molecular Informatics Center, Konkuk University) ;
  • Yi Yong-Sub (Seoul University of Venture and Information) ;
  • Choi Gyung-Ja (Screening Division, Korea Research Institute of Chemical Technology) ;
  • Cho Kwang-Yun (Screening Division, Korea Research Institute of Chemical Technology) ;
  • Lim Yoong-Ho (Bio/Molecular Informatics Center, Konkuk University)
  • Published : 2006.03.01

Abstract

Because of consumer rejection of chemical pesticides and the appearance of microorganisms that are resistant to fungicides, we tried to discover biopesticides. Of 13 microorganisms isolated from Shrimp-jeotkal, a Bacillus sp. showed strong activity against tomato late blight caused by Phytophthora infestans. Its activity was tested both in vivo and in vitro. The identification of the strain was carried out based on 16S rDNA analysis and the morphology by scanning electron microscopy.

Keywords

References

  1. Choi, S. H., C. Sung, and W. Y. Choi. 2001. Levan-producing Bacillus subtilis BS 62 and its phylogeny based on its 16S rDNA sequence. J. Microbiol. Biotechnol. 11: 428-434
  2. Goto, K., T. Omura, Y. Hara, and Y. Sadaie. 2000. Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J. Gen. Appl. Microbiol. 46: 1-8 https://doi.org/10.2323/jgam.46.1
  3. Hong, Y. S., H. S. Kim, and J. H. Lee. 2001. Molecular cloning and characterization of the doxA cytochrome P-450 hydroxylase gene in Streptomyces peucetius subsp caesius ATCC 27952. J. Microbiol. Biotechnol. 11: 895-898
  4. Judelson, H. S. 1997. The genetics and biology of Phytophthora infestans: Modern approaches to a historical challenge. Fungal Genet. Biol. 22: 65-76 https://doi.org/10.1006/fgbi.1997.1006
  5. Judelson, H. S., B. M. Tyler, and R. W. Michelmore. 1991. Transformation of the oomycete pathogen, Phytophthora infestans. Mol. Plant Microbe Interact. 4: 602-607 https://doi.org/10.1094/MPMI-4-602
  6. Kim, J. S., J. Y. Choi, J. H. Chang, H. J. Shim, J. Y. Roh, B. R. Jin, and Y. H. Je. 2005. Characterization of an improved recombinant baculovirus producing polyhedra that contain Bacillus thuringiensis Cry1Ac crystal protein. J. Microbiol. Biotechnol. 15: 710-715
  7. Kim, J., G. Choi, S. Lee, J. Kim, K. Chung, and K. Cho. 2004. Screening extracts of Achyranthes japonica and Rumex crispus for activity against various plant pathogenic fungi and control of powdery mildew. Pest Manag. Sci. 60: 803-808 https://doi.org/10.1002/ps.811
  8. Kim, K.-J., S.-H. Eom, S.-P. Lee, H.-S. Jung, S. Kamoun, and Y. S. Lee. 2005. A genetic marker associated with the A1 mating type locus in Phytophthora infestans. J. Microbiol. Biotechnol. 15: 502-509
  9. Lee, N. K., S.-A. Jun, J.-U. Ha, and H.-D. Paik. 2000. Screening and characterization of bacteriocinogenic lactic acid bacteria from jeot - gal, a Korean fermented fish food. J. Microbiol. Biotechnol. 10: 423-428
  10. Lee, S.-J., J.-Y. Cho, J.-I. Cho, J.-H. Moon, K. D. Park, Y. J. Lee, and K.-H. Park. 2004. Isolation and characterization of antimicrobial substance macrolactin A produced from Bacillus amyloliquefaciens CHO104 isolated from soil. J. Microbiol. Biotechnol. 14: 525-531
  11. Mah, J.-H., J.-B. Ahn, J.-H. Park, H.-C. Sung, and H.-J. Hwang. 2003. Characterization of biogenic amine-producing microorganisms isolated from myeolchi-jeot, Korean salted and fermented anchovy. J. Microbiol. Biotechnol. 13: 692- 699
  12. Park, M.-K., K.-H. Liu, Y.-H. Lim, Y.-H. Lee, H.-G. Hur, and J.-H. Kim. 2003. Biotransformation of a fungicide ethaboxam by soil fungus Cunninghamella elegans. J. Microbiol. Biotechnol. 13: 43-49
  13. Tabashnik, B. E., N. Finson, F. R. Groeters, W. J. Moar, M. W. Johnson, K. Luo, and M. J. Adang. 1994. Reversal of resistance to Bacillus thuringiensis in Plutella xylostella. Proc. Natl. Acad. Sci. USA 91: 4120-4124
  14. Zheng, H.-Z., Y.-W. Kim, H.-J. Lee, R.-D. Park, W.-J. Jung, Y.-C. Kim, S.-H. Lee, T.-H. Kim, and K.-Y. Kim. 2004. Quantitative changes of pr proteins and antioxidative enzymes in response to Glomus intraradices and Phytophthora capsici in pepper (Capsicum annuum L.) plants. J. Microbiol. Biotechnol. 14: 553-562