Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Culture Conditions on Astaxanthin Formation in Red Yeast Xanthophyllomyces dendrorhous Mutant JH1

  • Kim Jeong-Hwan (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Choi Seok-Keun (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Park Young-Sam (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Yun Cheol-Won (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Cho Won-Dai (Agricultural Cooperative College) ;
  • Chee Kew-Mahn (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Chang Hyo-Ihl (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University)
  • Published : 2006.03.01
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Abstract

The formation of astaxanthin by Xanthophyllomyces dendrorhous mutant JH1 depends on the culture conditions. Therefore, the effects of inoculation rate (1-5%, v/v) and medium compositions (various carbon and nitrogen sources) on cell growth and astaxanthin formation in X. dendrorhous mutant JH1 were investigated. Inoculation at 3% (v/v) was optimal for cell growth and astaxanthin formation. The most effective carbon source for cell growth and astaxanthin formation was glucose, and the best nitrogen source was yeast extract. The 3% (w/v) glucose and 0.2% (w/v) yeast extract showed the best effect on cell growth and astaxanthin formation, compared with others tested. The 3% glucose, 0.2% yeast extract, $0.15%\;KH_{2}PO_{4}$, $0.05%\;MgSO_4$, $0.01%\;MnSO_4$, and $0.01%\;CaCl_2$ were selected for cell growth and astaxanthin formation. Under the conditions selected, the maximum concentrations of cell and astaxanthin obtained after 168 h of cultivation were 5.43 g/l and 28.20 mg/l, respectively.

Keywords

References

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