Effect of Aminoethoxyvinylglycine Dipping Treatment on Ethylene Production and Cell Wall Composition of 'Tsugaru' Apple Fruits during Cold Storage

Aminoethoxyvinylglycine 침지처리가 '쓰가루' 사과의 저온저장중 에틸렌발생과 세포벽성분들의 변화에 미치는 영향

  • Kang In-Kyu (Department of Environmental Horticulture, Sangju National University) ;
  • Choi Cheol (Division of Plant Biosciences, Kyungpook National University) ;
  • Choi Dong-Geun (Division of Biological Resources Sciences, Chonbuk National University)
  • 강인규 (상주대학교 환경원예학과) ;
  • 최철 (경북대학교 원예학과) ;
  • 최동근 (전북대학교 생물자원과학부)
  • Published : 2006.03.01

Abstract

This study was conducted to determine the influence of postharvest dipping treatment with aminoethoxyvinylglycine (AVG) on ethylene production and composition of non-cellulosic neutral sugars in cell walls of 'Tsugaru' apple fruits during storage. Fruits were harvested on August 20, soaked in AVG 50 and 75 $mg L^{-1}$ solution for 5 minutes, and stored in cold storage chamber at $0{\pm}1^{\circ}C$ for 60 days. Fruit quality factor, ethylene productions, and cell wall component changes were investigated at 20 days interval. As a result, the fruit firmness and acid content were much higher in AVG treated fruits than those of untreated one during 60 days of cold storage. Ethylene production of AVG treated fruits was reduced to the level of 1/10 compared with untreated one. As to the change of non-cellulosic neutral sugars in the cell walls of 'Tsu- garu' fruits, the major sugar was arabinose and galactose in water, CDTA and $Na_2CO_3$ soluble fractions. The content of arabinose and galactose in untreated fruits increased as the softening of fruits was in progress, but the fruits treated with AVG showed a little change during storage, so it is predicted that these two cell wall compositional sugars were not solubilized by the treatment of AVG. Accordingly, the marketability of 'Tsu- garu' fruits could remarkably increase when soaking the fruits in AVG solution after harvest.

Keywords

References

  1. Abbott laboratory technical report. 1996. $ReTain^{TM}$, plant growth regulator soluble powder
  2. Abbott laboratory technical report. 1998. $ReTain^{R}$, plant growth regulator harvest the potential
  3. Bartley, I.M. 1977. A further study of ${\beta}$-galactosidase activity in apple ripening in storage. J. Exp. Bot. 28:943-948 https://doi.org/10.1093/jxb/28.4.943
  4. Bartley, I.M. and M. Knee. 1982. The chemistry of textual changes in fruit during storage. Food Chem. 9:47-58 https://doi.org/10.1016/0308-8146(82)90068-1
  5. Blakeney, A.B., P.J. Harris, R.T. Henry, and B.A. Stone. 1983. A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohyd. Res. 113:291-299 https://doi.org/10.1016/0008-6215(83)88244-5
  6. Boller, T., R.C. Herner, and H. Kende. 1979. Assay for and enzymatic formation of an ethylene precursor, 1-aminocyclopropane-1-carboxylic acid. Planta 145:293-303 https://doi.org/10.1007/BF00454455
  7. Brecht, J.K. and D.J. Huber. 1988. Products released from enzymatically active cell wall stimulate ethylene production and ripening in preclimacteric tomato fruit. Plant Physiol. 88:1037-1041 https://doi.org/10.1104/pp.88.4.1037
  8. Chun, J.P., M.S. Park, Y.S. Hwang, and J.C. Lee. 1997. Effect of AVG on preharvest drop and fruit quality in 'Tsugaru' apple. J. Kor. Soc. Hort. Sci. 38:147-152
  9. Cuillas-Iturralde A., I. Zarra, and E.P. Lorences. 1993. Metabolism of cell wall polysaccharides from persimmon fruit Pectin solubilization during fruit ripening occurs in apparent absence of polygalacturonase activity. Physiol. Plant. 89:369-375 https://doi.org/10.1111/j.1399-3054.1993.tb00168.x
  10. DeVeau E., K.C. Gross, D. Huber, and A. Watada. 1993. Degradation and solubilization of pectin by $\beta$-galactosidase purified from avocado mesocarp. Physiol. Plant. 87:279-285 https://doi.org/10.1111/j.1399-3054.1993.tb01731.x
  11. Fan X., S.M. Blankenship, and J.P. Mattheis. 1999. 1-Methylcyclopropene inhibits apple ripening. J. Amer. Soc. Hort. Sci. 124(6):690-695
  12. Huber, D.J. 1983. The role of cell wall hydrolases in fruit softening. Hort. Rev. 5:169-219
  13. Gross, KC. 1983. Changes in free galactose myo-inositol and other monosaccharides in normal and non-ripening mutant tomatoes. Phytochem. 22:1137-1139 https://doi.org/10.1016/0031-9422(83)80207-6
  14. Gross, K.C. and C.E. Sams. 1984. Changes in cell wall neutral sugar composition during fruit ripening: a species survey. Phytochem. 23:2457-2461 https://doi.org/10.1016/S0031-9422(00)84075-3
  15. Gross, K.C. and S.J. Wallner. 1979. Degradation of cell wall polysaccharides during tomato fruit ripening. Plant Physiol. 63:117-120 https://doi.org/10.1104/pp.63.1.117
  16. Kang, I.K., K.H. Chang, and J.K. Byun. 1998. Solubilization and depolymerization of pectic and neutral sugar polymers during ripening and softening in persimmon fruits. J. Kor. Soc. Hort. Sci. 39:51-54
  17. Kang, I.K., H.Y. Kim, H.J. Kweon, and J.K. Byun. 1999. Changes in ethylene production, respiration rates and cell wall hydrolase activities during storage of apples. J. Kor. Soc. Hort. Sci. 40:451-454
  18. Kang I.K. and J.K Byun. 2002. Effect of aminoethoxy- vinylglycine dipping treatment on storability of 'Tsugaru' apple fruits. J. Kor. Soc. Hort. Sci. 33:306-308
  19. Kim J.K., K.C. Gross, and T. Solomos. 1987. Characterization of the stimulation of ethylene production by galactose in tomato(Lycopersicon esculentum Mill.) fruit. Plant Physiol. 85:804-807 https://doi.org/10.1104/pp.85.3.804
  20. Park, M.Y., H.J. Kweon, I.K. Kang, and J.K. Byun. 1999. Effects of AVG application on harvest time extension and storability improvement in 'Tsugaru' apple. J. Kor. Soc. Hort. Sci. 40:577-580
  21. Pressey, R. 1983.${\beta}$-Galactosidase in ripening tomato. Plant Physiol. 71:132-135 https://doi.org/10.1104/pp.71.1.132
  22. Redgwell, R.J., L.D. Melton, and D.J. Brasch. 1990. Cell wall changes in kiwifruit following postharvest ethylene treatment. Phytochem. 29:399-407 https://doi.org/10.1016/0031-9422(90)85087-V
  23. Redgwell, R.J. 1991. Cell wall polysaccharides of kiwifruit(Actinidia deliciosa): effect of ripening on the structural features of cell wall materials. Carbohydrate Res. 209: 191-202 https://doi.org/10.1016/0008-6215(91)80156-H
  24. Roh, K.A., K.C. Son, B.C. In, and E.C. Sisler. 2000. Effect of 1-methylcyclopropene and ethylene on the action mechanism and ripening in banana. J. Kor. Soc. Hort. Sci. 41:526-530
  25. Rose, J.K.C., K.A., Hadafield, J.M. Labavitch, and A.B. Bennett. 1998. Temporal sequence of cell wall disassembly in rapidly ripening melon fruit. Plant Physiol., 177:345-361
  26. Shewfelt, A.L., V.A. payter, and J.J. Jen. 1971. Textural changes and molecular characteristics of pectin constituent in ripening peaches. J. Food Sci. 36:573-575 https://doi.org/10.1111/j.1365-2621.1971.tb15132.x
  27. Wallner, S.J. 1978. Apple fruit ${\beta}$-galactosidase and softening in storage. J. Amer. Soc. Hort. Sci. 103:364-366