Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Saponin Analysis and Red Ginseng Production using the Simplified Method of Korean Ginseng (Panax ginseng C.A.Meyer)

고려인삼(Panax ginseng C.A.Meyer)의 간이법에 의한 홍삼제조 및 사포닌 성분분석

  • In Jun-Gyo (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • Kim Eun-Jeong (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • Lee Bum-Soo (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • Park Myung-Han (Research Institute of Biotechnology, BioPia Co., Ltd) ;
  • Yang Deok-Chun (Dept. of Oriental Medicine Material and processing, Kyung Hee Univ.)
  • 인준교 ((주)바이오피아 생명공학연구소) ;
  • 김은정 ((주)바이오피아 생명공학연구소) ;
  • 이범수 ((주)바이오피아 생명공학연구소) ;
  • 박명한 ((주)바이오피아 생명공학연구소) ;
  • 양덕춘 (경희대학교 한방재료가공학과)
  • Published : 2006.02.01
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Abstract

In order to enhance the components of bioactive ginsenosides and the manufacturing process of red ginseng, we developed the simplified method for red ginseng production. The red ginseng extract was prepared from red ginseng produced with the simplified method, and the production rate of extract ($62^{\circ}$ brix) was more than 60%. The ginsenosides of red ginseng were purified and analyzed by HPLC using ELSD. Ginsenoside-$Rg_3,\;Rh_2$ and $Rh_1$, specific artifacts found only in red ginseng, were detected by HPLC. Especially, contents of ginsenoside-$Rg_3$ and Rh1 were detected high than two times in red ginseng produced the simplified method compared to commercial products.

홍삼 제조공정을 단순화하면서 효율적으로 유효성분을 증대시키기 위해서 4년근 수삼을 구입하여 깨끗이 수세한 후 $96-98^{\circ}C$에 3시간 정도 수증기로 증삼한 후 30시간 정도 열풍건조하여 홍삼제조를 위한 간이법을 개발하였다. 제조한 홍삼으로부터 홍삼엑스($60^{\circ}$ brix 이상)를 제조하였으며 홍삼엑스의 추출수율은 60% 이상이었다. 간이법으로 제조한 홍삼을 재료로 하여 농축한 홍삼엑스의 사포닌 함량을 조사하기 위해서 HPLC를 이용하여 총 사포닌(total saponin)을 분석하였다. 그 결과 PD계 사포닌 중 암세포전이억제효과와 평활근이완작용이 탁월한 ginsenoside-Rg3에는 H사에 비하여 2배 이상 검출되었으며, 간상해 억제작용과 혈소판 응집억제작용이 있는 것으로 알려진 ginsenoside-Rh1종의 경우 3배 이상 검출이 되었다.

Keywords

References

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