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The Korean Society of Phycology (한국조류학회(藻類))
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Morphology and Molecular Phylogeny of Hypnea flexicaulis(Gigartinales, Rhodophyta) from Korea

  • Geraldino, Paul John L. (Department of Biology, Chungnam National University) ;
  • Yang, Eun-Chan (Department of Biology, Chungnam National University) ;
  • Bu, Sung-Min (Department of Biology, Chungnam National University)
  • Published : 2006.12.31
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Abstract

Morphology and molecular phylogeny of a red algal species, Hypnea flexicaulis that is recently described from Japan, were investigated based on 23 collections from Korea (21), Taiwan (1), and the Philippines (1). Hypnea flexicaulis has percurrent axes with flexuous, antler-like branches which have wide branching angles, and abaxially curved ultimate branchlets. In order to study DNA divergence and phylogenetic relationships of the species, we determined plastid rbcL and mitochondrial cox1 sequences from the 23 collections. All 21 specimens from five different locations in Korea were almost identical to H. flexicaulis from Japan in rbcL sequences. Although there was a difference of three to five base pairs (bp) between samples from Korea and the Philippines or between the Philippines and Taiwan, Bayesian analyses of the rbcL data showed that all specimens from Korea, Japan, the Philippines, and Taiwan were strongly monophyletic. However, it is interesting that specimens from the Philippines differed by 31-34 base pairs in mitochondrial cox1 gene from those of materials from Korea and Taiwan, which differed by one to seven bp in rbcL between them. Although H. boergesenii is different from H. flexicaulis in having many antler-like branchlets, both appeared as sisters in all analyses of the rbcL data. This is the first report of H. flexicaulis from Korea based on morphology, rbcL, and cox1 gene sequences.

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References

  1. Burger G., Saint-Louis D., Gray M.W. and Lang B.F. 1999. Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea. Cyanobacterial introns and shared ancestry of red and green algae. Plant Cell 11: 1675-1694 https://doi.org/10.1105/tpc.11.9.1675
  2. Freshwater D.W. and Rueness J. 1994. Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species based on rbcL nucleotide sequence analysis. Phycologia 33: 187-194 https://doi.org/10.2216/i0031-8884-33-3-187.1
  3. Freshwater D.W., Fredericq S., Butler B.S., Hommersand M.H. and Chase M.W. 1994. A gene phylogeny of the red algae (Rhodophyta) based on plastid rbcL. Proc. Nat. Acad. Sci. U.S.A. 91: 7281-7285
  4. Gavio B. and Fredericq S. 2002. Grateloupia turuturu (Halymeniaceae, Rhodophyta) is the correct name of the non-native species in the Atlantic known as Grateloupia doryphora. Eur. J. Phycol. 37: 349-360 https://doi.org/10.1017/S0967026202003839
  5. Guiry M.D. et al. 2006. AlgaeBase version 4.1 World-wide Web electronic publication. National University of Ireland, Galway. http://www.algaebase.org; searched on 11 November 2006
  6. Haroun R.J. and Prud'Homme van Reine W.F. 1993. A biogeographical study of Laurencia and Hypnea species of the Macronesian region. Courier Forsch. Inst. Senkenberg. 159:119-125
  7. Hommersand M.H. and Fredericq S. 2001. Biogeography of the marine red algae of the South African West Coast: a molecular approach. Proc. Inter. Seaweed Symp. 17: 325-336
  8. Huelsenbeck J. and Ronquist F. 2003. MrBayes, Version 3.0. Evolutionary Biology Centre, Uppsala University, Uppasala
  9. Kraft G.T., Liao L.M., Millar A.J.K., Coppejans E.G.G., Hommersand M.H. and Freshwater D.W. 1999. Marine benthic red algae (Rhodophyta) from Bulusan, Sorsogon province, southern Luzon, Philippines. Philipp. Scient. 36: 1-50
  10. Lamouroux J.V.F. 1813. Essai sur les genres de la famille des thalassiophytes non articulées. Ann. Mus. Hist. Nat., Paris 20: 21-47, 115-139, 267-293, Plates 7-13. Notes: Reprinted as 22339
  11. Leblanc C., Boyen C., Richard O., Bonnard G., Grienenberger J.M. and Kloareg B. 1995. Complete sequence of the mitochondrial DNA of the rhodophyte Chondrus crispus (Gigartinales). Gene content and genome organization. J. Mol. Biol. 250: 484-495 https://doi.org/10.1006/jmbi.1995.0392
  12. Lee Y.P. and Kang S.Y. 2001. A catalogue of the seaweeds in Korea. Cheju National University Press, Jeju. 662 pp
  13. Lin S.M., Fredericq S. and Hommersand M.H., 2001. Systematics of the Delesseriaceae (Ceramiales, Rhodophyta) based on large subunit rDNA and rbcL sequences, including the Phycoryoideae, subfam. nov. J. Phycol. 37: 881-899 https://doi.org/10.1046/j.1529-8817.2001.01012.x
  14. Provan J., Murphy S. and Maggs C.A. 2004. Universal plastid primers for Chlorophyta and Rhodophyta. Eur. J. Phycol. 39: 43-50 https://doi.org/10.1080/09670260310001636668
  15. Robba L., Russell S.J., Barker G.L. and Brodie J. 2006. Assessing the use of the mitochondrial cox1 marker for use in DNA barcoding of red algae (Rhodophyta). Amer. J. Bot. 93: 1101-1108 https://doi.org/10.3732/ajb.93.8.1101
  16. Saunders G.W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Phil. Trans. R. Soc. B. 360: 1879-1888 https://doi.org/10.1098/rstb.2005.1719
  17. Shin W.G. and Boo S.M. 1994. A systematic study on the genus Hypnea (Gigartinales, Rhodophyta) in Korea. Korean J. Phycol. 9: 7-20
  18. Tanaka T. 1941. The genus Hypnea from Japan. Sci. Pap. Inst. Algol. Res., Fac. Sci. Hokkaido Univ. 2: 227-250
  19. Yamagishi Y. and Masuda M. 2000. A taxonomic revision of a Hypnea charoides-valentiae complex (Rhodophyta, Gigartinales) in Japan, with a description of Hypnea flexicaulis sp. nov. Phycol. Res. 48: 27-35 https://doi.org/10.1111/j.1440-1835.2000.tb00127.x
  20. Yamagishi Y., Masuda M., Abe T., Uwai S., Kogame K., Kawaguchi S. and Phang S.M. 2003. Taxonomic notes on marine algae from Malaysia. XI. Four species of Rhodophyceae. Bot. Mar. 46: 534-547 https://doi.org/10.1515/BOT.2003.056
  21. Yang E.C. and Boo S.M. 2004. Evidence for two independent lineages of Griffithsia (Ceramiaceae, Rhodophyta) based on plastid protein-coding psaA, and rbcL gene sequences. Mol. Phylogenet. Evol. 31: 680-688 https://doi.org/10.1016/j.ympev.2003.08.014
  22. Yang E.C. and Boo S.M. 2006. A red alga-specific phycoerythrin gene for biodiversity surveys of callithamnioid algae. Mol. Ecol. Notes 6: 533-535 https://doi.org/10.1111/j.1471-8286.2005.01216.x
  23. Yang H.N., Wang W.L. and Liao L.M. 1994. Marine algal flora of Pengchia Yu and its special place in the marine phytogeography of Taiwan. Bot. Mar. 37: 429-432 https://doi.org/10.1515/botm.1994.37.5.429
  24. Womersley H.B.S. 1998. The Marine Benthic Flora of southern Australia, Rhodophyta. Part IIIC. State herbarium of South Australia, South Australia. 535 pp
  25. Zuccarello G.C., Burger G., West J.A. and King R.J. 1999. A Mitochandrial marker for red algal intraspecific relationship. Mol. Ecol. 8: 1443-1447 https://doi.org/10.1046/j.1365-294x.1999.00710.x

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