Effects of Dietary Peroxidizability Index Values on Hepatic TBARS and Antioxidant Enzyme Activity in 7,12-dimethylbenz[$\alpha$]anthracene-treated Rats

  • Kang Min Jeong (Department of Food & Nutrition, College of Human Ecology, Hanyang University) ;
  • Shin Myoung Suk (Department of Food & Nutrition, College of Human Ecology, Hanyang University) ;
  • Park Tung Nan (Department of Food & Nutrition, College of Human Ecology, Hanyang University) ;
  • Lee Sang Sun (Department of Food & Nutrition, College of Human Ecology, Hanyang University)
  • Published : 2006.02.01

Abstract

Breast cancer may be the consequence of free radical damage, which is partially caused by the excessive intake of dietary fat and imbalances in antioxidant scavenger system;. In this experiment, we examined! the effects of dietary peroxidizability index (PI) values on hepatic thiobmbituric acid reaction substances (TBARS) and antioxidant enzyme activities in rats treated with 7,12-dimethylbenz[$\alpha$]anthracene (DMBA). Female Sprague-Dawley rats were used and 7,12-DMBA (20 mg/kg body weight) was gastrically intubated at seven weeks of age in order to induce mammary tumors (MT). The levels of dietary PI were 36, 81, 126 and 217 (LPI, MLPI, MHPI and HPI), while dietary polyunsaturated/saturated fatty acids ratio was maintained at the same level (1.0). Fat used in the experiment was mixed with soybean oil, com oil, palm oil, perilla oil, sesame oil, fish oil, and beef tallow. Experimental diets were given for the following 20 weeks. We measured tumor numbers and weights, and then assayed the hepatic TBARS levels and antioxidant enzyme activities such as superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione-S-transferase (GST) and glutathione reductase (GR). The incidence of Mr was the lowest in the MHPI group. The hepatic TBARS level was significantly raised with increasing dietary PI value. The hepatic SOD and GR activities were differed significantly by dietary PI value. The hepatic SOD activity was negatively correlated with dietary PI value and GR activity was the highest in the rats fed the MHPI diet. When the dietary P/S ratio is kept at 1.0, adequate dietary PI value (PI value of 126) may reduce the incidence and growth of Mr, but this benefit may be lost with higher dietary PI value. These results suggest that the awareness of dietary PI values may help to decrease breast cancer incidence and growth.

References

  1. Sies H. Strategies of antioxidant defense. Eur J Biochem 215:213-219, 1993 https://doi.org/10.1111/j.1432-1033.1993.tb18025.x
  2. Szczubial M, Kankofer M, Lopuszynski W, Dabrowski R, Lipko J. Oxidative stress parameters in bitches with mammary gland tumours. J Vet Med A Physiol Pathol Clin Med 51:336-340, 2004 https://doi.org/10.1111/j.1439-0442.2004.00647.x
  3. Escrich E, Solanas M, Soler M, Ruiz de Villa MC, Sanchez JA, Segura R. Dietary polyunsaturated n-6 lipids effects on the growth and fatty acid composition of rat mammary tumors. J Nutr Biochem 12:536-549, 2001 https://doi.org/10.1016/S0955-2863(01)00172-3
  4. Cutler RG. Human longevity and aging:possible role of reactive oxygen species. Ann N Y Acad Sci 621:1-28, 1991 https://doi.org/10.1111/j.1749-6632.1991.tb16965.x
  5. Sohal RS, Orr We.Relationship between antiocidants, prooxidants, and the aging process. Ann N Y Acd Sci 663:74-84, 1992 https://doi.org/10.1111/j.1749-6632.1992.tb38651.x
  6. Yang CS, Maliakal P, Meng X. Inhibition of carcinogenesis by tea. Annu Rev Pharmacol Toxicol 42:25-54, 2002 https://doi.org/10.1146/annurev.pharmtox.42.082101.154309
  7. Davis L, Kuttan G. Effect of Withania somnifera on DMBA induced carcinogenesis. J Ethnopharmacol 75:165-168, 2001 https://doi.org/10.1016/S0378-8741(00)00404-9
  8. Frenkel K. Carincogen-mediated oxidant formation and oxidative DNA damage. Pharmacol Ther 53:127-166, 1992 https://doi.org/10.1016/0163-7258(92)90047-4
  9. Actis AB, Cremonezzi DC, King IB, Joekes S, Eynard AR, Valentich MA. Effects of soy oil on murine salivary tumorigenesis. Prostaglandins Leukot Essent Fatty Acids 72:187-194, 2005 https://doi.org/10.1016/j.plefa.2004.10.020
  10. Franceschi S, Favero A, Decarli A, Negri E, La Vecchia C, Ferraroni M et al. Intake of macronutrients and risk of breast cancer. Lancet 347:1351-1356, 1996 https://doi.org/10.1016/S0140-6736(96)91008-9
  11. Key TJ, Allen NE, Spencer EA, Travis Re. The effect of diet on risk of cancer. Lancet 360:861-868, 2002 https://doi.org/10.1016/S0140-6736(02)09958-0
  12. Boyd NF, Martin L, Lockwood G, Greenberg C, Yaffe M, Tritchler D. Diet and breast cancer. Nutrition 14:722-724, 1998 https://doi.org/10.1016/S0899-9007(98)00058-6
  13. Willett WC. Specific fatty acids and risks of breast and prostate cancer:dietary intake. Am J Clin Nutr 66:1557S-1563S, 1997 https://doi.org/10.1093/ajcn/66.6.1557S
  14. Reeves PG. Components of the AlN-93 diets are improvements in the AIN-76 diet. J Nutr 127:838S-841S, 1997 https://doi.org/10.1093/jn/127.5.838S
  15. Kang MJ, Lee EK, Lee SS. Effects of two P/S ratios with Same peroxidizability index value and antioxidants supplementation on serum lipid concentration and hepatic enzyme activities of rats. Clin Chim Acta 350:79-87, 2004 https://doi.org/10.1016/j.cccn.2004.07.005
  16. Kang MJ, Lee EK, Lee SS. Polyunsaturated/saturated fatty acid ratios and antioxidant supplementation under the control of dietary peroxidizability index value:impact on serum lipid profiles in young and adult rats. Nutr Sci 8: 10-15, 2005
  17. Clinton SK, Alster JM, Imrey PB, Nandkumar S, Truex CR, Visek WJ. Effects of dietary protein, fat and energy intake during an initiation phase study of 7, 12-dimethylben[${\alpha}$]anthracene-induced breast cancer in rats. J Nutr 116:2290-2302, 1986 https://doi.org/10.1093/jn/116.11.2290
  18. Carroll KK. Dietary fats and cancer. Am J Clin Nutr 53:1064S-1067S, 1991 https://doi.org/10.1093/ajcn/53.4.1064S
  19. Fraga CG, Leibovitz BE, Tappel AL. Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med 4:155-161, 1988 https://doi.org/10.1016/0891-5849(88)90023-8
  20. Marklund S. Pyrogallol autoxidation. In CRC handbook of methods for oxygen radical research, p.243, CRC Press. Boca Raton, FL, 1984
  21. Aebi HE. Catalase. In:Bergmeyer H, Bergmeyer J, GraBl M, 3rd ed. Methods of enzymatic analysis, vol. 3, pp.273-285, Verlag Chemie CmbH. Weinheim, Germany, 1984
  22. Claiborne A. Catalase activity. In CRC handbook of methods for oxygen radical research, p.283, CRC Press. Boca Raton, FL, 1984
  23. Flohe L, Gunzler WA. Assays of glutathione peroxidase. Methods Enzymol 105:114-121, 1984 https://doi.org/10.1016/S0076-6879(84)05015-1
  24. Warholm M, Guthenberg C, von Bahr C, Mannervik B. Glutathione transferases from human liver. Methods Enzymol 113:499-504, 1985 https://doi.org/10.1016/S0076-6879(85)13065-X
  25. Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 113:484-490, 1985 https://doi.org/10.1016/S0076-6879(85)13062-4
  26. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254, 1976 https://doi.org/10.1016/0003-2697(76)90527-3
  27. Gammal EB, Carroll KK, Plunkett ER. Effects of dietary fat on mammary carcinogenesis by 7, 12-dimethylbenz(alpha) anthracene in rats. Cancer Res 27:1737-1742, 1967
  28. Costa I, Moral R, Solanas M, Escrich E. High-fat com oil diet promotes the development of high histologic grade rat DMBA-induced mammary adenocarcinomas, while high olive oil diet does not. Breast Cancer Res Treat 86:225-235, 2004 https://doi.org/10.1023/B:BREA.0000036896.75548.0c
  29. Tamimi RM, Hankinson SE, Spiegelman D, Colditz GA, Hunter DJ. Manganese superoxide dismutase polymorphism, plasma antioxidants, cigarette smoking, and risk of breast cancer. Cancer Epidemiol Biomarkers Prev 13:989-996, 2004
  30. Khaondze SS, Muddeshwar MG, Khanodze SD, Dakhlale GN. Antioxidant enzymes and lipid peroxidation in different stages of breast cancer. Free Radic Res 38:81-85, 2004 https://doi.org/10.1080/01411590310001637066
  31. Hu ML, Frankel EN, Leibovitz BE, Tappel AL. Effect of dietary lipids and vitamin E on in vitro lipid peroxidation in rat liver and kidney homogenates. J Nutr 119:1574-1582, 1989 https://doi.org/10.1093/jn/119.11.1574
  32. Ray G, Batra S, Shukla NK, Deo S, Raina V, Ashok S et al. Lipid peroxidation, free radical production and antioxidant status in breast cancer. Breast Cancer Res Treat 59: 163-170, 2000 https://doi.org/10.1023/A:1006357330486
  33. Du C, Sato A, Watanabe S, Wu CZ, Ikemoto A, Ando K et al. Cholesterol synthesis in mice is suppressed but lipofuscin formation is not affected by long-term feeding of n-3 fatty acid-enriched oils compared with lard and n-6 fatty acid-enriched oils. Biol Pharm Bull 26:766-770, 2003 https://doi.org/10.1248/bpb.26.766