Korean Journal of Biological Psychiatry (생물정신의학)

The Korean Society of Biological Psychiatry (대한생물정신의학회)
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Microarray Analysis of Gene Expression in Rat Hippocampus of Maternal Social Separation Model

모성 및 사회성 분리 백서 모델의 해마에서 유전자 칩을 이용한 유전자 발현 연구

  • Lee, Hee Jae (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Son, Chang Hee (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kwak, Hyong Ryol (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Lee, Sang-Hyun (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Han, Yoon Hee (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kim, Soo Young (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Park, Jong-Ik (Department of Psychiatry, College of Medicine, Kangwon National University) ;
  • Chun, Wanjoo (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University)
  • 이희제 (강원대학교 의과대학 약리학교실) ;
  • 손창희 (강원대학교 의과대학 약리학교실) ;
  • 곽형렬 (강원대학교 의과대학 약리학교실) ;
  • 이상현 (강원대학교 의과대학 약리학교실) ;
  • 한윤희 (강원대학교 의과대학 약리학교실) ;
  • 김수영 (강원대학교 의과대학 약리학교실) ;
  • 박종익 (강원대학교 의과대학 정신과학교실) ;
  • 전완주 (강원대학교 의과대학 약리학교실) ;
  • 김성수 (강원대학교 의과대학 약리학교실)
  • Published : 2006.05.31
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Abstract

Objectives : Alteration of hippocampus was demonstrated in the maternal social separation(MSS) pups, separated from dams on postnatal day(pnd) 14 and placed alone. Therefore, to understand the molecular events involved in the MSS, we have initiated a search for gene profiles that are up or down-regulated in the hippocampus of MSS pups. Methods : Analysis of cDNA microarray was performed by using total RNA extracted from the hippocampus of control and MSS pups on pnd 17. Also, passive-avoidance test was demonstrated on pnd 35. Results : Up-regulation of Nedd4a was observed in the hippocampus of MSS pups. Also, MSS rats showed less elongation of latency in passive avoidance test. Conclusion : We suggest that environmental effects of MSS may be altered the neural and/or glial differentiation and synapse formation-related genes which may lead cognitive alterations in MSS rats.

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