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Development of Simultaneous Analysis for the Multi-residual Pesticides in the Ginseng Extract using Gas Chromatography

인삼농축액에서 GC를 이용한 잔류농약 동시다성분 분석법의 개발

  • 신영민 (부산지방식품의약품안전청) ;
  • 이선화 (부산지방식품의약품안전청) ;
  • 손영욱 (부산지방식품의약품안전청) ;
  • 정지윤 (식품의약품안전청 잔류물질화학팀) ;
  • 정성욱 (인제대학교 환경공학부) ;
  • 박흥재 (인제대학교 환경공학부) ;
  • 김성훈 (영산대학교 동양조리학과) ;
  • 원영준 (부산지방식품의약품안전청) ;
  • 이창희 (부산지방식품의약품안전청) ;
  • 김우성 (부산지방식품의약품안전청) ;
  • 홍무기 (식품의약품안전청 잔류물질화학팀) ;
  • 채갑용 (부산지방식품의약품안전청)
  • Published : 2006.01.01

Abstract

The simultaneous analysis of multi-residual pesticides was developed using a gas chromatography (GC) method. In this study, a simple and reliable methodology was improved to detect 154 kinds of pesticides in sinseng extract sample by using a liquid-liquid extraction procedure, open column chromagraphy and chromatographic analysis by CC electron capture detector (ECD) and GC nitrogen-phosphorus detector (NPD). The 154 kinds of pesticides were classified in 4 groups according to the chemical structure. The extraction of pesticides was experimented with $70\%$ acetone and dichloromethane/petroleum ether in order, and cleaned up via open column chromatography $(3\times30cm)$ packed with florisil $(30g,\;130^{\circ}C,\;12hrs)$. The final extract was concentrated in a rotator evaporator at $40^{\circ}C$ until dryness. Then the residue was redissolved to 2ml with acetone, and analyzed by GC-ECD and GC-NPD. The applied concentration of pesticides was over $1\~10{\mu}g/ml$. The recovery tests were ranged from $70.7\%$ to $115.2\%$ with standard deviations between 0.3 and $5.7\%$ of the standard spiked to the ginseng extract sample (Group $I\~IV$). The limit of detection (LOD) ranged from 0.001 to $0.099{\mu}g/ml$ (Group $I\~IV$). The 9 kinds of pesticides were not detected. The developed method was applied satisfactory to the determination of the 154 kinds of pesticides in the ginseng extract with good reproducibility and accuracy.

Keywords

References

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