Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Transformation of Leuconostoc mesenteroides SY1, a Strain Isolated from Kimchi

  • JEONG SEON-JU (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • PARK JAE-YONG (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • KIM JONG HWAN (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • KIM GYEONG MIN (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • CHUN JIYEON (Division of Applied Life Science, Graduate School,Gyeongsang National University) ;
  • LEE JONG-HOON (Department of Food Science and Biotechnology, Kyonggi University) ;
  • CHUNG DAE-KYUN (School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • KIM JEONG HWAN (Institute of Agriculture & Life Science, Gyeongsang National University)
  • Published : 2006.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Leuconostoc mesenteroides SY1, a strain isolated from cabbage Kimchi, was transformed with pCW4, a shuttle vector based on a cryptic plasmid from Lactobacillus paraplantarum C7. $\alpha-Amylase$ gene, amyL, from Bacillus licheniformis was cloned into pCW4, resulting in $pCW4T{\alpha},\;and\;pCW4T{\alpha}$ was introduced into SY1 by electroporation. Transformation efficiency was $10^2cells/{\mu}g$ plasmid DNA. L. mesenteroides cells harboring $pCW4T{\alpha}$ did not show amylase activity, although amyL transcript was synthesized as determined by slot blot experiment. $pCW4T{\alpha}$ was stably maintained in SY1 in the presence of erythromycin (Em, $5\;{\mu}g/ml$) but rapidly lost when Em was omitted. Less than $1\%$ of the cells maintained $pCW4T{\alpha}$ after 5 days at $30^{\circ}C$.

Keywords

References

  1. Berthier, F., M. Zagorec, M. Champomier-Verges, S. D. Ehrlich, and F. Morel-Deville. 1996. Efficient transformation of Lactobacillus sake by electroporation. Microbiology (Reading) 142: 1273-1279 https://doi.org/10.1099/13500872-142-5-1273
  2. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16: 6127-6145 https://doi.org/10.1093/nar/16.13.6127
  3. Kiewiet, R., J. Kok, J. F. Seegers, G. Venema, and S. Bron. 1993. The mode of replication is a major factor in segregational plasmid instability in Lactococcus lactis. Appl. Environ. Microbiol. 48: 726-731
  4. Kim, I. C., S. Y. Jang, J. H. Cha, Y. H. Ko, K. H. Park, and H. M. Rho. 1988. Cloning and expression of thermostable ${\alpha}$-amylase gene in Escherichia coli from Bacillus licheniformis ATCC 27811. Kor. J. Appl. Microbiol. Bioeng. 16: 369-373
  5. Kim, J. H. and S. H. Woo. 1995. Expression of Bacillus licheniformis ${\alpha}$-amylase gene in Lactobacillus casei strains. J. Microbiol. Biotechnol. 5: 257-263
  6. Kim, S. J., J. H. Kim, J. Y. Park, H. T. Kim, S. J. Jung, Y. L. Hae, H. D. Yun, and J. H. Kim. 2004. Cold adaptation of Lactobacillus paraplantarum C7 isolated from Kimchi. J. Microbiol. Biotechnol. 14: 1071-1074
  7. Kim, Y. C., E. Y. Jung, E. H. Kim, D. H. Jung, S. H. Jung, D. H. Yi, T. J. Kwon, and S. M. Kang. 1998. Properties of acid tolerance of acid-resistant mutant Leuconostoc mesenteroides which was improved as Kimchi starter. Kor. J. Appl. Microbiol. Biotechnol. 26: 102-109
  8. Min, S. K., J. H. Kim, S. Kim Cho, H. S. Shin, G. H. Hong, D. G. Oh, and K. N. Kim. 2003. Manufacturers of functional Kimchi using Bifidobacterium strain producing conjugated linoleic acid (CLA) as starter. Kor. J. Food Sci. Technol. 35: 111-114
  9. Moon, G. S., C. H. Kang, Y. R. Pyun, and W. J. Kim. 2004. Isolation, identification, and characterization of a bacteriocin-producing Enterococcus sp. from Kimchi and its application to Kimchi fermentation. J. Microbiol. Biotechnol. 14: 924- 931
  10. O'Sullivan, D. J. and T. R. Klaenhammer. 1993. Rapid mini-prep isolation of high quality plasmid DNA from Lactococcus and Lactobacillus spp. Appl. Environ. Microbiol. 59: 2730- 2733
  11. Park, M. S., H. W. Moon, and G. E. Ji. 2003. Molecular characterization of plasmid from Bifidobacterium longum. J. Microbiol. Biotechnol. 13: 457-462
  12. Park, W. J., K. H. Lee, J. M. Lee, H. J. Lee, J. H. Kim, J.-H. Lee, H. C. Chang, and D. K. Chung. 2004. Characterization of pC7 from Lactobacillus paraplantarum C7 derived from Kimchi and development of lactic acid bacteria-Escherichia coli shuttle vector. Plasmid 52: 84-88 https://doi.org/10.1016/j.plasmid.2004.05.001
  13. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A