Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Synergistic Effect of Staphylococcus aureus and LPS on Silica-Induced Tumor Necrosis Factor Production in Macrophage Cell Line J774A.1

  • LEE DONG HEE (The Research Institute of Natural Science, Sookmyung Women's University) ;
  • PARK BONG JOO (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • LEE MIN SUB (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • CHOI JAE BONG (Division of Industrial and Systems Engineering, Hansung University) ;
  • KIM JEONG KOO (Department of Biomedical Engineering, College of Biomedical Science and Engineering, Inje University) ;
  • PARK JONG HOON (The Research Institute of Natural Science, Sookmyung Women's University) ;
  • PARK JONG-CHUL (Department of Medical Engineering, Yonsei University College of Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine)
  • Published : 2006.01.01
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Abstract

In this study, we investigated the synergistic effects of Staphylococcus aureus extracts (membranes and walls) and lipopolysaccharide (LPS) derived from Escherichia coli on tumor necrosis factor (TNF) production in the pathogenesis of silica-induced inflammation. The synergistic induction of TNF by silica particles $(<20\;{\mu}m)$ in combination with either S. aureus extracts or LPS was examined in J774A.1 cell cultures. Media from the treated and untreated cell cultures were assayed for TNF, using the mouse WEHI 164 cell cytotoxicity assay and enzyme immunoassay. Cells exposed simultaneously to silica and $0.5\;{\mu}g/ml$ S. aureus extracts (or 0.5 ng/ml of LPS) produced a significantly higher level of TNF than those produced by the inducer alone. Our results indicate that device-associated infections (or pyrogen contamination) could enhance inflammatory responses, because of particles produced by the wear of medical implants or particulate biomaterials used for clinical purposes.

Keywords

References

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