Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidant Potential of Enzymatic Extracts from Blueberry (Vaccinium corymbosum L.)

블루베리 (Vaccinium corymbosum L.) 유래 효소 추출물의 항산화성

  • Senevirathne Mahinda (Department of Food Bioengineering, Cheju National University) ;
  • Jeon You-Jin (Faculty of Applied Marine Science, Cheju National University) ;
  • Ha Jin-Hwan (Department of Food Bioengineering, Cheju National University) ;
  • Cho Somi K. (Faculty of Biotechnology, Cheju National University) ;
  • Kim Soo-Hyun (Department of Food Bioengineering, Cheju National University)
  • ;
  • 전유진 (제주대학교 해양생산과학부) ;
  • 하진환 (제주대학교 식품공학과) ;
  • 김소미 (제주대학교 생명공학부) ;
  • 김수현 (제주대학교 식품공학과)
  • Published : 2006.02.01
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Abstract

Enzymatic extracts were prepared from the blueberry (Vaccinium corymbosum L.) collected in Jeju, Korea. Five carbohydrases namely AMG, Celluclast, Termamyl, Ultraflo and Viscozyme, and five proteases namely Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex were used to prepare the enzymatic extracts. Antioxidant properties of each extracts were studied using stable 1,1-diphenyl 2-picrylhydrazyl (DPPH), reactive oxygen species (ROS), nitric oxide (NO) scavenging, metal chelating assays and lipid peroxidation inhibitory activity in hemoglobin-induced linoleic acid system. The phenolic content of all enzymatic extracts was in the range of 517.85-597.96 mg/100 g dried sample. DPPH and NO${\cdot}$scavenging, and metal chelating assays exhibited prominent activities. Viscozyme showed the highest DPPH activity $(0.046{\pm}0.002\;mg/mL)$ while AMG Showed the highest activity in NO${\cdot}$scavenging $(0.339{\pm}0.011\;mg/mL)$. All the extracts exhibited strong metal chelating activities. Blueberry enzymatic extracts also showed relatively good activity in hydrogen peroxide scavenging. AMG showed the highest lipid peroxidation inhibitory activity $(0.28{\pm}0.01\;mg/mL)$ in hemoglobin-induced linoleic acid system. In this results, the blueberry, which has potential antioxidant components, may be a good candidate as a natural antioxidant source.

제주지역에 서식하고 있는 블루베리 (Vaccinium corymbosum L.)로부터 수용성 추출물을 효과적으로 제조하기 위하여 5가지 종류의 탄수화물 분해효소 (AMG, Celluclast, Termamyl, Ultraflo, Viscozyme)와 또 다른 5종의 단백질 분해효소 (Alcalase, Flavourzyme, Kojizyme, Neutrase, Protamex)를 이용하여 효소적 추출을 시도하였다. 이들 효소를 이용하여 제조된 여러 가지 블루베리 효소 추출물에 대하여 1,1-diphenyl 2-picrylhydrazyl (DPPH), 활성산소종 (ROS), 일산화질소 $(NO{\cdot})$ 등의 소거 활성, 금속 킬레이트결합능, 그리고 지질 과산화 저해능과 같은 항산화 효과를 검토하였다. 모든 효소 추출물의 페놀함량은 $517.85\~597.96\;mg/100 g$ 건조시료이었으며, 항산화 효과에 대한 평가에서 특히 DPPH 및 $NO{\cdot}$ 소거활성, 그리고 금속 킬레이트 결합능 등이 우수한 것으로 나타났다. Viscozyme 추출물은 DPPH 소거활성 $(0.046{\pm}0.002\;mg/mL)$이, 그리고 AMG 추출물은 $NO{\cdot}$ 소거활성$(0.339{\pm}0.011\;mg/mL)$ 및 지질 과산화 억제활성 $(0.28{\pm}0.01\;mg/mL)$이 각각 우수하였다. 과산화 수소 소거활성에 있어서는 블루베리 효소 추출물들이 천연 항산화제인 a-토코페롤보다 상대적으로 높았다. 이러한 결과로 볼 때 블루베리는 항산화 효과가 우수한 화합물들을 함유하고 있을 것으로 판단되며, 향후 천연 항산화 자원으로서 이용이 가능할 것으로 사료된다.

Keywords

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