Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Kinetics for Citric Acid Production from the Concentrated Milk Factory Waste Water by Aspergillus niger ATCC 9142

Aspergillus niger ATCC 9142 세포에 의해 농축된 우유공장폐수로부터 구연산생산에 대한 동력학 연구

  • Lee Yong-Hee (Research Institute for Basic Sciences, Dong-Eui University) ;
  • Suh Myung-Gyo (Department of Medical Administration, Dong-Eui Institute of Technology) ;
  • Chung Kyung-Tae (Department of Life Science and Biotechnology, Dong-Eui University)
  • 이용희 (동의대학교 기초과학연구소) ;
  • 서명교 (동의과학대학 의무행정과) ;
  • 정경태 (동의대학교 자연과학대학 생명응용과학)
  • Published : 2006.02.01
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Abstract

The waste water from a milk factory was investigated for possibility of use to the production of citric acid by cells of Aspegillus niger ATCC 9142. The addition of $Mn^{2+},\;Fe^{2+}\;and\;Cu^{2+}$ ions to waste increased citric acid production steadily, but addition of metal ion $Mg^{2+}$ decreased the citric acid production. The amount of produced citric acid by Aspegillus niger ATCC 9142 with addition 50 g/1 and 100 g/1 of reducing sugar in milk factory waste water were 7.2 g/1 and 16.5 g/1 respectively. Mathematical model was simulated for their predictability of cell growth, citric acid production and substrate consumption rate and coincided with experimental data.

Aspegillus niger 세포에 의한 구연산 생산에 대한 발효 매체로서 우유공장폐수가 연구되었다. 발효매체에 $Mn^{2+},\;Fe^{2+}\;and\;Cu^{2+}$의 첨가가 구연산 생산을 일정하게 증가시켰다. 그러나 다른 금속이온인 $Mg^{2+}$의 첨가는 구연산 생산을 감소시켰다. 구연산의 농도는 우유공장폐수에서 환원당 농도의 농도가 50 g/l와 100 g/1로 Aspegillus niger ATCC 9142에 의한 batch bioreactor에서 각각 7.2 g/1과 16.5 g/l로 높게 표시되었다. 본 실험에서 적용한 수리적 모델식들이 세포성장의 예상, 구연산 생산, 기질소모 속도에 대해 잘 적용되는지를 알아보기 위해서 발효반응 모델식에 적용시켜 본 결과 실험치와 잘 부합되었다.

Keywords

References

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