Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Morphological, Phylogenetic and Biological Characteristics of Ectropis obliqua Single-Nucleocapsid Nucleopolyhedrovirus

  • Ma Xiu-cui (Institute of Insect Sciences, Zhejiang University) ;
  • Xu Hai-Jun (Institute of Insect Sciences, Zhejiang University) ;
  • Tang Mei-Jun (Tea Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Xiao Qiang (Tea Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Hong Jian (Institute of Insect Sciences, Zhejiang University) ;
  • Zhang Chuan-Xi (Institute of Insect Sciences, Zhejiang University)
  • Published : 2006.02.01
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Abstract

The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to $1.7\;{\mu}m$ in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rrl, polyhedrin, orfl629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration $(LC_{50}\;and\;LC_{90})$ and lethal time $(LT_{50}\;and\;LT({90})$ were conducted to test the susceptibility of E. obliqua larvae to the virus.

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References

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