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황석어(Collichthys nireatus Jordan et starks) 젓갈의 숙성과정 중 품질변화와 항산화작용에 관한 연구

Studies on Quality Changes and Antioxidant Activity During the Fermentation of the Salt Fermented Whangseoke

  • 김지상 (경희대학교 식품영양학과) ;
  • 문갑순 (인제대학교 식품과학연구소 식품생명과학부, 바이오헬스소재연구센터) ;
  • 이경희 (경희대학교 외식산업학과) ;
  • 이영순 (경희대학교 식품영양학과)
  • Kim, Ji-Sang (Dept. of Food and Nutrition, Kyung-Hee University) ;
  • Moon, Gap-Soon (Fond Science Institute, School of Food and Life Science, and Biohealth Products Research Center, Inje University) ;
  • Lee, Kyung-Hee (Dept. of Food Service Management, Kyung-Hee University) ;
  • Lee, Young-Soon (Dept. of Food and Nutrition, Kyung-Hee University)
  • 발행 : 2006.02.01

초록

본 연구는 황석어 젓갈을 $25^{\circ}C$에서 숙성시키면서 숙성기간(240일)에 따른 품질변화와 항산화 작용에 미치는 영향을 조사하여 그 특성을 규명하였다. 숙성기간에 따른 황석어 젓갈의 산가는 숙성 60일 이후 산가가 급격하게 증가하여 숙성초기 3.63 mg/g였던 산가가 숙성 240일에는 84.03 mg/g 으로 크게 증가하였으며 과산화물가는 숙성기간 180일까지 증가하여 347.53 meq/kg으로 나타났으나 그 이 후 감소하여 240일째 185.64 meq/kg으로 나타났다. TBA가는 숙성초기 1.28 mg/kg이었던 TBA가가 숙성 240일째에는 14.89 mg/kg 으로 나타났다. Linoleic acid emulsion system계를 이용한 황석어 젓갈의 항산화작용을 살펴본 결과, 과산화물가는 모든 시료가 incubation 기간 $6\~9$일 사이에 과산화물가가 급격히 증가하였으며, 숙성 180일의 젓갈 첨가군$(0\~16.50\;meq/kg)$의 항산화효과가 가장 높았으며 240일 숙성 젓갈의 경우 prookidant 작용을 하는 것을 알 수 있었다. DPPH에 의한 radical 소거 작용을 살펴 본 결과 숙성기간이 증가함에 따라 라디칼 소거능이 증가하여 숙성초기 $44.27\%$였던 소거능이 240일 숙성젓갈에서 $63.83\%$로 나타났다. 환원력도 숙성기간에 따라 증가하여 숙성초기 0.09였던 환원력이 240일째 0.15로 나타났다. 따라서 황석어 젓갈은 숙성기간이 증가함에 따라 라디칼소거능, 환원력이 증가하면서 항산화활성이 증가하는 것으로 나타났지만 어류 자체의 산패 증가로 항산화효과는 숙성 180일째에 가장 높음을 알 수 있었다. 숙성기간에 따른 갈색도 변화는 흡광도 400/500 nm에 대한 흡광 비율을 측정한 결과 숙성초기 2.49였던 흡광도 비율은 숙성 240일째 3.42로 숙성기간에 따라 증가하는 것으로 나타났다. 숙성 초기 1.38 mg/mL였던 환원당 함량은 숙성 240일에는 0.53 mg/mL으로 숙성기간이 길어짐에 따라 감소하였다. 이 상의 결과 숙성 기간에 따라 DPPH에 의한 전자공여성, 환원력, 갈색도 등의 증가, 환원당의 감소, linoleic acid emulsion system계에서 젓갈 첨가군이 대조군보다 항산화 효과가 있는 것으로 나타나 Maillard 반응생성물에 의한 항산화 물질들이 형성되어 젓갈의 항산화성이 나타난 것으로 여겨진다. 젓갈에는 고도불포화지방산이 많이 함유되어 있으나 어유의 산화를 방지하는 물질 역시 많이 생성되며 이들이 젓갈의 산패를 어느 정도 억제하는 것으로 추정된다.

The effect of storage temperature on the quality and antioxidative activity Whangseoke sauce was studied over a period of 240 days. Fermented Whangseoke with $25\%$ salt were stored at $25^{\circ}C$. The quality change and antioxidant activity of Whangseoke in linoleic acid emulsion was evaluated with various parameters including acids values, peroxide values, TBA values, reducing sugar, brown color intensity, electron donating ability and reducing power at various time intervals for 240 days of storage. In general, it was observed, in all sample, that peroxide values, brown color intensity, electron donating ability and reducing power gradually increased, while reducing sugar decreased during storage at $25^{\circ}C$. The antioxidative activities of fermented Whangseoke were determined on tile linoleic acid emulsion system. The results showed that Whangseoke had antioxidant activity. These results suggest that antioxidant activity of Whangseoke seemed to influence by Maillard reaction products during the storage periods.

키워드

참고문헌

  1. Suh HK. 1987. A Study on the regional characteristics of Korean Chotkal. Korea J Food Culture 2: 149-161
  2. Lee WD. 2001. Recent development of Jeotagal (traditional Korean fermented seafood) and its future. Food Industry and Nutrition 6: 23-27
  3. Do SD, Lee YM, Chang HG. 1993. The study on kinds and utilities of Jeot-Kal (fermented fish products). Korean J Food Cookery Sci 9: 222-229
  4. Kim SH. 2002. Development of seasoned whangseoke-jeot with chitosan. Korean J Food Cookery Sci 18: 34-42
  5. Valenzuela A, Nieto S, Cassels BK, Speisky H. 1991. Inhibitory effect of boldine on fish oiloxidation. J Am Oil Chem Soc 68: 935-937 https://doi.org/10.1007/BF02657538
  6. Marcusk R. 1960. Antioxidative effect of amino acids. Nature 185: 886-887
  7. Murase H, Nagao A, Terao J. 1993. Antioxidant and emul-sifying action of N-(long-chain-acyl)histidine and N- (long-chain-acyl)carnosine. J Agric Food Chem 41: 1601- 1604 https://doi.org/10.1021/jf00034a014
  8. Wade AM, Turker HN. 1998. Antioxidant characteristics of L-histidine. J Nutr Biochem 9: 308-315 https://doi.org/10.1016/S0955-2863(98)00022-9
  9. Lingnert H, Eriksson CE. 1981. Antioxidative Maillard reaction products I. Products from sugars and free amino acids. Prog Food Nutr Sci 5: 453-466
  10. Shahidi F, Amarowicz R. 1996. Antioxidant activity of protein hydrolyzates from aquatic species. J Am Oil Chem Soc 73: 1197-1199 https://doi.org/10.1007/BF02523384
  11. Folch J, Lee M, Sloan Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509
  12. Wrolstad RE, Acree TE, Decker EA. 2005. Handbook of food analytical chemistry: pigments, colorants, flavors, texture, and bioac-tive food components. John Wiley & Sons Inc, New Jersey
  13. Pikul J, Leszcyzynski DE, Kummerow FA. 1983. Elimination of sample autooxidation by butylated hydr-oxy-toluene additions before thiobarbituric assay for malo-n-aldehyde in fat from chicken meat. J Agric Food Chem 31: 1338-1342 https://doi.org/10.1021/jf00120a047
  14. Hayase F, Kato H. 1984. Antioxidative components of sweet potatoes. J Nutr Sci Vitaminol 30: 37-46 https://doi.org/10.3177/jnsv.30.37
  15. Blois MS. 1958. Antioxidant determination by the use of stable free radical. Nature 26: 1199-2000
  16. Oyaizu M. 1986. Antioxidant activity of browning products of glucosamine fractionated by organic solvent and thinlayer chromatography. Nippon Shokuhin Kogyo Gakkaishi 35: 771-775
  17. Lee YS, Homma S, Aida K. 1987. Characterization of melanoidin in soy sauce and fish sauce by electrofocusing and high performance gel permeation chromatography. Nippon Shokuhin Kogyo Gakkaishi 5: 313-319
  18. Miller GL. 1959. Use of reagent for determination of re-ducing sugar. Anal Chem 31: 426-428 https://doi.org/10.1021/ac60147a030
  19. Gokhan B, Hikmet K, Muhammet B. 2005. Change in the quality of fish oils due to storage temperature and time. Food Chemistry (article on line) 29: 1-6 https://doi.org/10.1016/0308-8146(88)90070-2
  20. Lee YC. 1990. Measurement of rancidity of edible fats and oils. J Korean Oil Chemists' Soc 7: 91-100
  21. Song YO, Byeun DS, Byeun JH. 1982. Lipid oxidation and proteolysis of anchovy pickle during ripening. J Korean Soc Food Nutr 11: 1-6
  22. Lee EH, Cho SY, Cha YJ, Jeon JK, Kim SK. 1981. The effect of antioxidants on the fermented Sardine and taste com-pounds of product. J Kor Fish Soc 14: 201-211
  23. Mukai FH, Goldstein BD. 1976. Mutagenicity of malonal-dehyde, a decomposition product of polyunsturated fatty acids. Science 191: 868-869 https://doi.org/10.1126/science.766187
  24. Newburg DS. 1980. Malonaldehyde concentrations in food are affected by cooking condition. J Food Science 45: 1681- 1683 https://doi.org/10.1111/j.1365-2621.1980.tb07588.x
  25. Siu GM. 1978. A survey of the malonaldehyde content of retail meats and fish. J Food Sci 43: 1147-1148 https://doi.org/10.1111/j.1365-2621.1978.tb15256.x
  26. Tanaka M, Kuie CW, Nagashima Y, Taguchi T. 1988. Application of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi 54: 1409-1414 https://doi.org/10.2331/suisan.54.1409
  27. Duh PD. 1998. Antioxidant activity of burdock (Ar-ctium lappa Linn). Its scavenging effect on free radical and active oxygen. J Am Oil Chem Soc 75: 1214-1218
  28. Gordon MF. 1990. The mechanism of antioxidant action in vitro. In Food antioxidants. Hudson BJF, ed. Elsevier Applied Science, London. p 1-18
  29. You BJ, Lee KH, Kim CY, Lee JH. 1986. Antioxidant ac-tivity of amino acid-xylose browning reaction products. 1. Anti-oxidant activity various amino acids and their brown-ing reaction products. Bull Korean Fish Soc 19: 1-9
  30. Kiligaya N, Kato H, Fujimaki M. 1968. Studies on anti-oxidant activity of nonenzymatic browning reaction products. Part I. Relations of color intensity and reductones with antioxidant activity of browning reaction products. J Agric Chem Soc 32: 289-290
  31. Lingnert H, Eriksson CE. 1981. Antioxidative effect of Maillard reaction products. Food Nutr Sci 5: 453-466
  32. Cho Y, Rhee HS. 1979. A study on flavorous taste com-po-nents in Kimchis on free amino acids. Korean J Food Sci Technol 11: 26-31
  33. Park WP, Kim ZU. 1991. The effectof spices on the Kimchi fermentation. J Korean Soc Appl Biol Chem 34: 235-241

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