Piggery Waste Treatment using Partial Nitritation and Anaerobic Ammonium Oxidation

부분질산화와 혐기성 암모늄산화를 이용한 돈사폐수처리

  • Hwang, In-Su (Office of Livestock Wastewater Treatment Plant, Sanju city) ;
  • Min, Kyung-Sok (Department of Environmental Engineering, Kyungpook National University) ;
  • Lee, Young-Ok (Division of Life Science, Daegu University)
  • 황인수 (상주시축산폐수처리사업소) ;
  • 민경석 (경북대학교 환경공학과) ;
  • 이영옥 (대구대학교 생명과학부)
  • Received : 2005.11.15
  • Accepted : 2006.04.06
  • Published : 2006.07.30

Abstract

Nitrogen removal with the combined SHARON (Single reactor system for high ammonium removal over nitrite)ANAMMOX (Anaerobic ammonium oxidation) process using the effluent of ADEPT (Anaerobic digestion elutriated phased treatment) slurry reactor with very low C/N ratio for piggery waste treatment was investigated. For the preceding SHARON reactor, ammonium nitrogen loading and removal rate were $0.97kg\;NH_4-N/m^3_{reactor}/day$ and $0.68kg\;NH_4-N/m^3_{reactor}/day$ respectively. In steady state, bicarbonate alkalinity consumption for ammonium nitrogen converted to $NO_2-N$ or $NO_3-N$ was 8.4 gram per gram ammonium nitrogen. The successive ANAMMOX reactor was fed with the effluent from SHARON reactor. The loading and removal rate of the soluble nitrogen defined as the sum total of $NH_4-N$, $NO_2-N$ and $NO_3-N$ in ANAMMOX reactor were $1.36kg\;soluble\;N/m^3_{reactor}/day$ and $0.7kg\;soluble\;N/m^3_{reactor}/day$, respectively. The average $NO_2-N/NH_4-N$ removal ratio by ANAMMOX was 2.41. Fluorescence in situ hybridization (FISH) analysis verified that Candidatus Kuenenia stuttgartiensis were dominate, which means that they played an important role of nitrogen removal in ANAMMOX reactor.

Keywords

References

  1. Ahn, Y. H., Hwang, I. S. and Min, K. S., ANAMMOX and Partial Denitritation In Anaerobic Nitrogen Removal Treating Piggery Waste, Wat. Sci. Tech., 49(5/6), pp. 145-154 (2004)
  2. APHA, WEF and ASCE, Standard Methods for the Examination of Water and Wastewater, 20th Eds., Washington DC. U.S.A. (1998)
  3. Fux, C., Boehler, M., Philipp, H., Brunner, I. and Siegrist, H., Biological Treatment of Ammonium-rich Wastewater by Partial Nitritation and Subsequent Anaerobic Ammonium Oxidation (anammox) in a Pilot Plant, J. of Biotechnol., 99, pp. 295-306 (2002) https://doi.org/10.1016/S0168-1656(02)00220-1
  4. Hwang, I. S., Min, K. S. and Lee, Y. O., Nitrogen Removal from Piggery Wastewater using ANAMMOX Reactor, Proc. of Anaerobic Digestion 2004 10th WORLD CONGRESS, 29, Aug.-2 Sep., Montreal, Canada (2004)
  5. Min, K. S., Ahn, Y. H., Hwang, I. S. and Choi, E., Feasibility of Ammonium Removal in Anaerobic Sludge Bed Reactor Teating Piggery Waste, Proc. of Animal residuals 2002 Conference and Workshop, Washington DC. U.S.A., 6-8 May, 11(3), (2002)
  6. Mobarry, B. K., Wagner, M., Urbain, V., Rittmann, B. E. and Stahl, D. A., Phylogenetic Probes for Analyzing Abundance and Spatial Organization of Nitrifying Bacteria, Appl. Environ. Microbiol., 62, pp. 2156-2162 (1996)
  7. Neef, A., Amann, R. I., Schlesner, H. and Schleifer, K. H., Monitoring a Widespread Bacterial Group: In Situ Detection of Planctomycetes with 16S rRNA-targetted Probes, Microbiology (UK), 144, pp. 3257-3266 (1998) https://doi.org/10.1099/00221287-144-12-3257
  8. Pynaert, K., Smets, B. F., Wyffels, S., Beheydt, D., Siciliano, S. D. and Verstraete, W., Characterization of an Autotrophic Nitrogen-removing Biofilm from a Highly Loaded Lab-scale Rotation Biological Contactor, Appl. Environ. Microbiol., 69, pp. 3626-3635 (2003) https://doi.org/10.1128/AEM.69.6.3626-3635.2003
  9. Schmid, M., Twachtmann, U., Klein, M., Strous, M., Juretschko, S., Jetten, M., Metzger, J. W., Schleifer, K. H. and Wagner, M., Molecular Evidence of Genus Level Diversity of Bacteria Capable of Catalyzing Anaerobic Ammonium Oxidation, System. Appl. Microbiol., 23, pp. 93-106 (2000) https://doi.org/10.1016/S0723-2020(00)80050-8
  10. van Dongen, L. G. J. M., Jetten, M. S. M. and van Loosdrecht M. C. M., The Combined Sharon/Anammox Process. STOWA Report, TWA Publishing, London, UK. (2001)
  11. Wagner, M., Rath, G., Koops, H. P., Floos, J. and Amann, R., In Situ Analysis of Nitrifying Bacteria in Sewage Treatment Plants, Wat. Sci. Tech., 34, pp. 237-244 (1996)