Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Characteristics of the extracellular products (ECPs) of Vibrio harveyi grown under various conditions

배양 조건에 따른 Vibrio harveyi extracellular products (ECPs)의 특성

  • Published : 2006.08.01
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Abstract

The characteristics of the extracellular products (ECPs) of Vibrio harveyi grown under various conditions were studied. The extracellular products (ECPs) of Vibrio harveyi is well known as an important pathogenic factor. The optimal isolation condition of the V. harveyi ECPs was incubation with 1.5% NaCl added TSA medium at 25℃ for 24 h. The buffer system for ECPs isolation controlled as pH 8.0 displayed optimal condition. The major protein of ECPs isolated from the five V. harveyi strains originated from Korea (FF8, FF10, FR1, FR2 and FT1 strain) were revealed to serine protease.

본 연구에서는 Vibrio harveyi의 주요 병원성 인자로 알려져 있는 ECPs를 분리하는 적정 조건을 제시하고자 하였다. 5 개의 분리균주 (FF8, FF10, FR1, FR2 and FT1 strain)와 2 개의 참조균주 (ATCC 35084 및 ATCC 14126)의 ECPs를 다양한 조건에서 분리해 본 결과, 1.5% NaCl 첨가 TSA 배지에서 25℃, 24 시간 배양하는 것이 가장 좋으며, ECPs 분리시의 buffer system은 pH 8.0이 적정한 것으로 나타났다. 나아가 우리 나라에서 분리한 5 개의 분리 균주들의 주요 ECPs 성분이 serine protease인 것으로 조사되었다.

Keywords

References

  1. Austin, B., Austin, D.A., Dalsgaard, I., Gudmundsdottir, B.K., Hoie, S., Thornton, J.M., Larsen, J.L., O'Hici, B. and Powell, R.: Characterization of atypical Aeromonas salmonicida by different methods. Syst. Appl. Microbiol., 21: 50-64, 1998 https://doi.org/10.1016/S0723-2020(98)80008-8
  2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254, 1976 https://doi.org/10.1016/0003-2697(76)90527-3
  3. Farto, R., Perez, M.J., Fernandez-Briera, A. and Nieto, T.P.: Purification and partial characterization of a fish lethal extracellular protease from Vibrio pelagius. Vet. Microbiol., 89: 181-194, 2002 https://doi.org/10.1016/S0378-1135(02)00172-4
  4. Harris, L.J. and Owens, L.: Production of exotoxins by two luminous Vibrio harveyi strains known to be primary pathogens of Penaeus monodon larvae. Dis. Aquat. Org., 38: 11-22, 1999 https://doi.org/10.3354/dao038011
  5. Jiravanichpaisal P., Miyazaki, T. and Limsuwan, C.: Histopathology, biochemistry, and pathogenicity of Vibrio harveyi infecting black tiger prawn Penaeus monodon. J. Aquat. Anim. Health., 6: 27-35, 1994 https://doi.org/10.1577/1548-8667(1994)006<0027:HBAPOV>2.3.CO;2
  6. Lavilla-Pitogo C.R., Baticados, M.C.L., Cruz-Lacierda, E.R. and De la Pena, L.D.: Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines. Aquaculture, 91: 1-13, 1990 https://doi.org/10.1016/0044-8486(90)90173-K
  7. Lee, K.K., Liu, P.C., and Chuang, W.H.: Pathogenesis of gastroenteritis caused by Vibrio carchariae in cultured marine fish. Mar. Biotechnol., 4: 267-277, 2002 https://doi.org/10.1007/s10126-002-0018-9
  8. Liu, P.C. and Lee, K.K.: Cysteine protease is a major exotoxin of pathogenic luminous Vibrio harveyi in the tiger prawn, Penaeus monodon. Letters Appl. Microbiol., 28: 428-430, 1999 https://doi.org/10.1046/j.1365-2672.1999.00555.x
  9. Liu, P.C., Lee, K.K., Yii, K.C., Kou, G.H. and Chen, S.N.: Isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus. Curr. Microbiol., 33: 129-132, 1996 https://doi.org/10.1007/s002849900087
  10. Liu, P.C., Chuang, W.H. and Lee, K.K.: Infectious gastroenteritis caused by Vibrio harveyi (V. carchariae) in cultured red drum, Sciaenops ocellatus. J. Appl. Ichthyol., 19: 59-61, 2003 https://doi.org/10.1046/j.1439-0426.2003.00356.x
  11. Liu, P.C., Lee, K.K., Tu, C.C. and Chen, S.N.: Purification and characterization of a cysteine protease produced by pathogenic luminous Vibrio harveyi. Curr. Microbiol., 35: 32-39, 1997 https://doi.org/10.1007/s002849900207
  12. Montero, A.B. and Austin, B.: Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased postlarval Penaeus vannamei (Bonne). J. Fish Dis., 22: 377-386, 1999 https://doi.org/10.1046/j.1365-2761.1999.00189.x
  13. Munro, J., Oakey, J., Bromage, E. and Owens, J.: Experimental bacteriophage-mediated virulence in strains of Vibrio harveyi. Dis. Aquat. Org., 54: 187-194, 2003 https://doi.org/10.3354/dao054187
  14. Owens, L., Austin, D.A. and Austin, B.: Effect of strain origin on siderophore production in Vibrio harveyi isolates. Dis. Aquat. Org., 27: 157-160, 1996 https://doi.org/10.3354/dao027157
  15. Pedersen, K., Verdonck, L., Austin, B., Austin, DA, Blanch, A.R., Grimont, P.A.D., Jofre, J., Koblavi, S., Larsen, J.L., Tiainen, T., Vigneulle, M. and Swings, J.: Taxonomic evidence that Vibrio carchariae Grimes et al. 1985 is junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al. 1981. Int. J. Syst. Bacteriol., 48: 749-758, 1998 https://doi.org/10.1099/00207713-48-3-749
  16. Soffientino, B., Gwaltney, T., Nelson, D.R., Specker, J.L., Mauel, M. and Gomez-Chiarri, M.: Infectious necrotizing enteritis and mortality caused by Vibrio carchariae in summer flounder Paralichthys dentatus during intensive culture. Dis. Aquat. Org., 38: 201-210, 1999 https://doi.org/10.3354/dao038201
  17. Won K.M.: Characteristics of non-luminous Vibrio harveyi isolated from maricultured fishes in Korea. Ph. D. Thesis, Pukyong National University, Korea, 2005. (in Korean)
  18. Yii, K.C., Yang, T.I. and Lee, K.K.: Isolation and characterization of Vibrio carchariae, a causative agent of gastroenteritis in the groupers, Epinephelus coioides. Curr. Microbiol., 35: 109-115, 1997 https://doi.org/10.1007/s002849900221
  19. Zhang, X.H. and Austin, B.: Pathogenicity of V. harveyi to salmonids. J. Fish Dis., 23: 93-102, 2000 https://doi.org/10.1046/j.1365-2761.2000.00214.x
  20. Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez-Manzanares, E., Balebona, M.C. and Morinigo, M.A.: Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. J. Fish Dis., 26: 103-108, 2003 https://doi.org/10.1046/j.1365-2761.2003.00437.x