Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지

Chemical Constituents of Domestic Quercus spp. Leaves

국내산 참나무속 수종 잎의 추출성분

  • Kim, Jin-Kyu (Dept. of Wood Science & Engineering, College of Forest Science, Kangwon National University) ;
  • Bae, Young-Soo (Dept. of Wood Science & Engineering, College of Forest Science, Kangwon National University)
  • 김진규 (강원대학교 산림과학대학 임산공학과) ;
  • 배영수 (강원대학교 산림과학대학 임산공학과)
  • Received : 2006.09.25
  • Accepted : 2006.11.01
  • Published : 2006.11.25
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Abstract

This study was carried out to investigate chemotaxonomical correlation an d the chemical constituents of domestic Quercus sp. leaves. The leaves of Q. mongolica, Q. aliena, Q. serrata, Q. acutissima, Q. dentata and Q. variabilis were collected in the experimental forest of Kangwon National University. The combined extracts were successively fractionated with n -hexane, methylene chloride and ethyl acetate using a separation funnel. A portion of the ethyl acetate and $H_2O$ soluble materials of each species were chromatographed on a Sephadex LH-20 column using various aqueous MeOH and EtOH-hexane as washing solvents. Spectrometric analysis such as NMR and MS, including TLC, were performed to characterize the structures of the isolated compounds. Gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, kaempferol, astragalin, astragalin-6"-O-gallate, isoquercitrin, isoquercitrin-6"-O-gallate and myricetin were isolated from Q. mongolic a leaves. Gallic acid, kaempferol and quercetin were characterized from Q. acutissima leaves. Gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, (-)-epigallocatechin, kaempferol, quercetin, guajaverin and tamarixin were identified from Q. dentata leaves. Gallic acid, (+)-catechin, (-)-epicatechin, kaempferol, quercitrin, isoquercitrin and myricetin were purified from Q. serrata leaves. Gallic acid, (+)-catechin, astragalin, astragalin-6"-O-gallate and isoquercitrin were isolated from Q. variabilis leaves. Gallic acid was isolated from all the leaves and could be a taxonomic index on Quercus spp..

국내산 주요 참나무류인 신갈나무, 상수리나무, 떡갈나무, 졸참나무, 갈참나무 및 상수리나무 잎의 추출성분의 구조를 규명하고 수종 상호간의 성분의 특성 및 상호 연관성 등을 조사하였다. 신갈나무에서는 gallic acid, (+)-catechin, (-)-epicatechin, (+)-gallocatechin, kaempferol, a stragalin, astragalin-6"-O-gallate, isoquercirin, isoquercitrin-6"-O-gallate 및 myricetin 등 10종의 화합물이 단리되었고 상수리나무에서는 gallic acid, kaempferol과 quercetin이 떡갈나무에서는 gallic acid, (+)-catechin, (-)-epicatechin, (+)-galocatechin, (-)-epigallocatechin, kaempferol, quercetin, guajaverin 및 tamarixin 등 9종의 화합물이 단리되었으며 갈참나무에서는 gallic acid, caffeic acid, astragalin, quercetin과 isoquercitrin이 졸참나무에서는 gallic acid, (+)-catechin, (-)-epicatechin, kaempferol, quercitrin, isoquercitrin 및 myricetin 등 7종의 화합물이 단리되었고 굴참나무에서는 gallic acid, (+)-catechin, astragalin, astagalin-6"-O-gallate와 isoquercitrin 등 5종의 화합물이 단리되었다. Gallic aicd는 모든 수종에서 단리되어 참나무류 잎 추출성분의 지표 화합물로 이용될 수 있다.

Keywords

References

  1. 권오근, 김성환, 천병열, 박채규, 손건호. 1999. 목단피로 부터 항균활성 성분의 분리. 생약학회지 30: 340-344
  2. 김민영, 김윤근, 김태홍, 조종수, 양재경. 2000 떡갈나무 추출물의 항균활성 빚 항산화활성. 목재공학 28(3); 42-51
  3. 김석중, 윤광섭, 박희성. 2005. 송화분, 참나무 및 백합 화분 추출물의 항산화 효능. 한국식품과학회지.37(5); 833-837
  4. 김진규, 이상극, 함연호, 배영수. 2002. 상수리나무 (Quercus acutissima)와 굴참나무(Quercus variabilis) 수피의 추출성분. 임산에너지 21(1): 41-48
  5. 사전령, 김진규, 권동주, 배영수. 2006. 오동나무 열매 추출물의 페놀성 화합물. 목재공학 34(1): 79-85
  6. 신두호, 조정순. 1991 상수리(상橡實)의 각종 용매 추출 물이 linoleic acid의 항산화력에 미치는 영향. 한국유화학회지. 8(1): 79-83
  7. 임업연구원 1990. 참나무 資源의 綜合利用 開發에 關한 硏究(III). 과학기술처 특정개발사업 연구보고서
  8. 정선채, 황방연, 오갑진, 강신정, 김미정, 최우희, 이경순, 노재섭. 1999. 붉나무 수피의 성분. 생약학회지. 30: 295-300
  9. Collins, F. W., A. B. Bruce, and K. W. Cornelius. 1975. Flavonol glycoside gallates from Tellima grandtflora. Phytochemistry. 14(4): 1099-1102 https://doi.org/10.1016/0031-9422(75)85195-8
  10. Da Silva, B. P, R. R. Bernardo, and J. P Parente. 2000. Flavonol glycosides from Costus spicatus Phytochemistry 53: 87-92 https://doi.org/10.1016/S0031-9422(99)00441-0
  11. Do, J. C., K. Y. Jung, and K. H. Son 1992. Flavonoid glycosides from the fronds of Pyrrosia lingua. Kor. J. Pharmacogn. 23(4): 276-279
  12. Harbone, J. B. and T. J. Marby. 1982. The Flavonoids: Advanced in Reserch. pp. 19-134
  13. Kim, H. Y. B. H. Moon, H. J. Lee, and D. H Choi. 2004. Flavonol glycosides from the leaves of Eucommia ulmoides O. with glycation inhibitory activity. Journal of Ethnopharmacology 93: 227-230 https://doi.org/10.1016/j.jep.2004.03.047
  14. Liu, Y., Y. Wu, K. Yuan, C. Ji, A Hou, T. Yoshida, and T. Okuda 1997. Astragalin 2'', 6''-di-O-gallate from Loropetalum Chinense. Phytochemistry. 46(2): 389-391 https://doi.org/10.1016/S0031-9422(97)00295-1
  15. Norris, F. A. and F. R. Stermitz. 1970. 4'-O- methylquercetin 3-glucoside from Astragalus miser var oblongifolius Phytochemistry. 9(1) : 229-230 https://doi.org/10.1016/S0031-9422(00)86635-2
  16. Park, W. Y., S. C. Lee, B. T. Ahn, S. H. Lee, J. S. Ro, and K. S. Lee. 1993. Phenolic compounds from Acalypha australis L. Kor. J. Pharmacogn 24: 20-25
  17. Yamanaka, M, K. Shimomura, K. Sasaki, K. Yoshihira, and K. Ishimaru 1995. Glucosylation of Phenolics by Hairy Root Cultures of Lobelia Sessilifolia. Phytochemistry. 40(4): 1149-1150 https://doi.org/10.1016/0031-9422(95)00456-H